Colton H.F. Wong, David K.K. Leung, Francis P.W. Tang, Jenny K.Y. Wong, Nola H. Yu, Terence S.M. Wan
Abstract:Liquid chromatography/mass spectrometry (LC/MS) has been successfully applied to the detection of anabolic steroids in biological samples. However, the sensitive detection of saturated hydroxysteroids, such as androstanediols, by electrospray ionisation (ESI) is difficult because of their poor ability to ionise. In view of this, chemical derivatisation has been used to enhance the detection sensitivity of hydroxysteroids by LC/MS. This paper describes the development of a sensitive ultra-high-performance liqui…
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