Rapid Screening Method in the Identification of 17 Adulterants in Dietary Supplements

Polar licit and illicit ingredients in dietary supplements: chemometric optimization of extraction and HILIC-MS/MS analysis

“…In fact, it has already been proved that HILIC and RPLC can be considered as orthogonal separation strategies when dealing with doping-related studies [ 33 ]. Finally, the greenness of these four methodologies was estimated through the AGREE software, and the SALLE procedure developed in this work obtained a slightly higher score than the other studies: 0.74 [this work], 0.70 [ 9 ], 0.68 [ 14 ], 0.66 [ 64 ]. The detailed scorings are reported in Figure S.4 (A–D).…”

“…This SALLE methodology was then compared with other studies concerning the extraction of compounds from DS (including at least one analyte in common). Regarding the amount of sample employed for each replicate, there are studies that require less [ 9 ], more [ 64 ], or even exactly the same quantity [ 14 ] of DS. All these works evaluated the content of a variable quantity of compounds in about a dozen of DS.…”

“…Still, among the considered methods, our proposed SALLE procedure is the quickest and simplest one, involving less than a quarter of an hour to process a sample, doubling [ 14 , 64 ] or increasing about tenfold [ 9 ] the productivity of the procedure and avoiding long times of ultrasonication extractions [ 14 , 64 ], or laborious manual procedures like solid-phase extractions [ 9 ]. From a chromatographic point of view, some methods are quicker [ 9 , 14 ] and others slower [ 64 ], also depending on the specific separation strategy and the specificity of the detector employed. In fact, it has already been proved that HILIC and RPLC can be considered as orthogonal separation strategies when dealing with doping-related studies [ 33 ].…”