Rapid screening assay of congenital adrenal hyperplasia by measuring 17α‐hydroxyprogesterone with high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

Lai, Chien‐Chen; Tsai, Chang Hai; Tsai, Fuu Jen; Wu, Jer Yuarn; Lin, Wei; Lee, Cheng Chun

doi:10.1002/jcla.2039

Cited by 32 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other methods based on gas chromatography/mass spectrometry and fluorescence detection, although offering highly specific and sensitive analysis, require derivatization (Frerichs and Tornatore, 2004). Bioanalytical methods based on LC/MS/MS represent the most specific and sensitive methods for steroid analysis (Lai et al, 2002). This technique coupled with solid-phase extraction combines the attributes of rapidity, easy processing, accuracy, specificity, sensitivity, and precision without the need for sample derivatization.…”

Section: Discussionmentioning

confidence: 99%

Betamethasone Pharmacokinetics After Two Prodrug Formulations in Sheep: Implications for Antenatal Corticosteroid Use

Samtani

Löhle²,

Grant³

et al. 2005

Drug Metab Dispos

View full text Add to dashboard Cite

ABSTRACT:Maternal administration of betamethasone to enhance fetal lung maturation for women who threaten preterm labor is common clinical practice. However, recommendations regarding the choice of betamethasone formulations for perinatal use are vague. The disposition of betamethasone from two commonly used antenatal formulations is poorly understood. We therefore designed a study to capture the true pharmacokinetic profiles of betamethasone from these fast acting and dual-release formulations. Betamethasone in sheep plasma was measured by a newly designed, highly sensitive liquid chromatography/tandem mass spectrometry assay after intramuscular injection (n ‫؍‬ 4) of 0.25 mg/kg betamethasone phosphate and 0.5 mg/kg betamethasone phosphate/acetate formulations. Compartmental modeling was performed using the ADAPT II program. Betamethasone pharmacokinetics could be captured for 24 h for the phosphate and for 5 days for the phosphate/acetate formulations. The phosphate formulation profile had the appearance of a traditional Bateman function with a terminal half-life of 4 h, whereas the phosphate/acetate formulation produced a biexponential decline with a terminal half-life of 14 h. The latter is much longer than is commonly reported and has been missed in the literature due to assay limitations. Extrapolations to humans indicate that although both formulations might have similar therapeutic indices, the dual formulation might be associated with a lower safety profile. In light of this newly identified long terminal half-life for the betamethasone dual formulation, dosing practices for betamethasone in pregnancy need to be reassessed.Preterm birth occurs in about 10% of pregnancies, and complications associated with prematurity, especially respiratory distress syndrome, are the leading cause of mortality in prematurely born infants (NIH Consensus Panel, 1995). Betamethasone is administered maternally to enhance fetal lung maturation in women who threaten preterm labor during 24 to 34 weeks gestation. The National Institutes of Health recommends administration of two maternal intramuscular injections of 12-mg betamethasone 24 h apart for this condition. Although the doses of betamethasone are stated, the exact formulation recommendation is not clear. Betamethasone is available as a fast releasing phosphate ester prodrug formulation and as a dual acting suspension formulation containing phosphate and acetate ester prodrugs. Both formulations have been tested in clinical trials and have been shown to be efficacious in producing precocious fetal lung maturation (Liggins and Howie, 1972;Gamsu et al., 1989). However, there is controversy regarding the betamethasone-releasing properties of the acetate prodrug, and a recent meta-analysis suggests that this prodrug is probably of little therapeutic benefit for antenatal use (Jobe and Soll, 2004). Although the release properties of the acetate prodrug have been questioned, long-duration studies looking at the release pattern of betamethasone from this prodrug do not exist. ...

show abstract

Section: Discussionmentioning

confidence: 99%

Betamethasone Pharmacokinetics After Two Prodrug Formulations in Sheep: Implications for Antenatal Corticosteroid Use

Samtani

Löhle²,

Grant³

et al. 2005

Drug Metab Dispos

View full text Add to dashboard Cite

show abstract

“…For example, acylcarnitines are unstable if stored at room temperature for more than 2 weeks on DBS due to hydrolysis, but they are stable for at least 330 days when stored at ≤−18°C . Without establishing the analyte storage stability coverage (from sample collection to analysis), a retrospective reanalysis of the study samples might not be confi rmative for any purpose, especially for newborn screening, for which the confi rmatory analysis (secondtier test) is often conducted, but only after the initial population screening Lai et al, 2001Lai et al, , 2002la Marca et al, 2007la Marca et al, , 2008aMatern et al, 2007). Stability assessment.…”

Section: Stabilitymentioning

confidence: 99%

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

538

428

View full text Add to dashboard Cite

The collection of whole blood samples on paper, known as dried blood spot (DBS), dates back to the early 1960s in newborn screening for inherited metabolic disorders. DBS off ers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS off ers simpler sample collection and storage and easier transfer, with reduced infection risk of various pathogens, and requires a smaller blood volume. To date, DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecules. Despite the increasing popularity of DBS-LC-MS/MS, the method has its limitations in assay sensitivity due to the small sample size. Sample quality is often a concern. Systematic assessment on the potential impact of various blood sample properties on accurate quantifi cation of analyte of interest is necessary. Whereas most analytes may be stable on DBS, unstable compounds present another challenge for DBS as enzyme inhibitors cannot be conveniently mixed during sample collection. Improvements on the chemistry of DBS card are desirable. In addition to capturing many representative DBS-LS-MS/MS applications, this review highlights some important aspects of developing and validating a rugged DBS-LC-MS/MS method for quantitative analysis of small molecules along with DBS sample collection, processing and storage.

show abstract

“…In the current study, we have followed an analogous methodology whereby steroids with a 3␤-hydroxy-⌬ 5 structure have been converted to their 3-oxo-⌬ 4 oxidation products by treatment with cholesterol oxidase [35][36][37], and then reacted with Girard P (GP) reagent to give GP hydrazones [38,39]. Girard hydrazones possess a quaternary nitrogen and are readily analyzed by electrospray (ES) mass spectrometry [35][36][37][38][39][40][41][42][43][44][45]. Furthermore, tandem mass spectrometry (MS/MS) provides high sensitivity and structural information for GP hydrazone analysis.…”

mentioning

confidence: 99%

Analysis of oxysterols by electrospray tandem mass spectrometry

Griffiths

Wang

Alvélius

et al. 2006

J. Am. Soc. Mass Spectrom.

104

102

View full text Add to dashboard Cite

Oxysterols are oxygenated derivatives of cholesterol. They are intermediates in cholesterol excretion pathways and may also be regarded as transport forms of cholesterol. The introduction of additional hydroxyl groups to the cholesterol skeleton facilitates the flux of oxysterols across the blood brain barrier, and oxysterols have been implicated in mediating a number of cholesterol-induced metabolic effects. Oxysterols are difficult to analyze by atmospheric pressure ionization mass spectrometry on account of the absence of basic or acidic functional groups in their structures. In this communication, we report a method for the derivatization and analysis of oxysterols by electrospray mass spectrometry. Oxysterols with a 3␤-hydroxy-⌬ 5 structure were converted by cholesterol oxidase to 3-oxo-⌬ 4 steroids and then derivatized with the Girard P reagent to give Girard P hydrazones, which were subsequently analyzed by tandem mass spectrometry. The improvement in sensitivity for the analysis of 25-hydroxycholesterol upon oxidation and derivatization was over 1000. (J Am Soc Mass Spectrom 2006, 17, 341-362)

show abstract

Rapid screening assay of congenital adrenal hyperplasia by measuring 17α‐hydroxyprogesterone with high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

Cited by 32 publications

References 23 publications

Betamethasone Pharmacokinetics After Two Prodrug Formulations in Sheep: Implications for Antenatal Corticosteroid Use

Betamethasone Pharmacokinetics After Two Prodrug Formulations in Sheep: Implications for Antenatal Corticosteroid Use

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Analysis of oxysterols by electrospray tandem mass spectrometry

Contact Info

Product

Resources

About