Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates

Kiyohara, Hohzoh; Nagao, Keiko; Yana, Keiji

doi:10.1128/aem.43.2.454-457.1982

Cited by 325 publications

(94 citation statements)

References 16 publications

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“…Growth correspondmg to an OD,,, value of 0.2 or more was regarded as evidence of positive naphtalene degradation. The ability to degrade phenanthrene was detected by clearing of the opaque water-insoluble phenanthrene (Sigma) sprayed on HCMM2 agar plates as described by Kiyohara et al [25].…”

Section: Polyaromatic Hydrocarbon (Pah) Degradation Testsmentioning

confidence: 99%

Species variation and plasmid incidence among fluorescent Pseudomonas strains isolated from agricultural and industrial soils

Campbell

Jacobsen

Sørensen

2006

FEMS Microbiology Ecology

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A total of 132 different fluorescent Pseudomonas strains were isolated from several agricultural and industrial soils. The bacteria from the two different soil environments were compared for species and biotype variation, antibiotic and heavy metal resistance profiles, ability to degrade polyaromatic hydrocarbons, and plasmid incidence. Irrespective of the soil type, the isolates belonged to Pseudomonas fluorescens biotypes I–VI and Pseudomonas putida biotype B. Except for a streptomycin resistant isolate from one of the industrial soils, all the strains had the same antibiotic resistance profile. However, there was a higher incidence of heavy metal resistance and polyaromatic hydrocarbon degradation phenotypes in the isolates from industrial soils than from the agricultural soils. Only 2 out of 68 strains from agricultural soil were found to carry plasmids, while 28 out of 64 strains from industrial soil had plasmids. A majority of the plasmids (56%) were estimated to be larger than 50 kb, indicating that they could encode transfer functions. However, transferability as indicated by the ability to mobilize an IncQ plasmid (tra−, mob+), was observed with only one plasmid. None of the plasmid(s) containing isolates hybridized to a 32P‐labelled repP probe suggesting that none of the indigenous plasmids in the soil fluorescent Pseudomonas strains was related to the IncP group of conjugative plasmids commonly associated with resistance and catabolic genes.

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Section: Polyaromatic Hydrocarbon (Pah) Degradation Testsmentioning

confidence: 99%

Species variation and plasmid incidence among fluorescent Pseudomonas strains isolated from agricultural and industrial soils

Campbell

Jacobsen

Sørensen

2006

FEMS Microbiology Ecology

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“…Plate dilutions were performed on PAH-coated agarose plates made of the mineral salts medium M9 [30], containing 7 g agarose per liter and enriched with 2.5 ml/L trace elements [31]. The M9-plates were coated with phenanthrene (PHE) (Sigma, St. Louis, MO, USA) or pyrene (PYR) (Sigma) by a method modified after Kiyohara et al [32]. One milliliter of acetone stock solution (4 mg/ml) of either PHE or PYR was transferred to M9 plates.…”

Section: Enumeration Of Polycyclic Aromatic Hydrocarbondegrading Bactmentioning

confidence: 99%

Bioaugmentation of Tar-Contaminated Soils Under Field Conditions Using Pleurotus Ostreatus Refuse From Commercial Mushroom Production

Hestbjerg¹,

Willumsen²,

Christensen³

et al. 2003

Environ Toxicol Chem

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The influence of the white rot fungus Pleurotus ostreatus on the degradation of selected poly- and heterocyclic aromatic hydrocarbons (referred to as polycyclic aromatic hydrocarbons [PAHs]) in soil was investigated under field conditions representing the Northern temperate zone. Pleurotus ostreatus was added to two contaminated soils in the form of homogenized refuse from the commercial production of fungus. The soils were collected from a former shipyard (the B&W soil) and underneath a former coal tar storage at an old asphalt factory in Denmark (the Ringe soil). Treatments (control, soil mixed with autoclaved sawdust medium, and soil mixed with P. ostreatus refuse) were set up in triplicate in concrete cylinders (height, 50 cm; diameter, 60 cm). The activity of P. ostreatus was measured as laccase activity and phenanthrene (PHE)- and pyrene (PYR)-degrading bacteria were enumerated. Twenty-one different PAHs were quantified. After nine weeks the concentrations of the 3-, 4-, 5-, and 6-ring PAHs in the Ringe soil were reduced by 78, 41, and 4%, respectively. These reductions corresponded with high initial laccase activity, a decrease in pH caused by the fungus, and an increase in the number of PHE- and PYR-degrading bacteria. No significant PAH degradation was observed in the B&W soil. Reasons for the difference in performance of P. ostreatus in the two soils are discussed in terms of soil histories and bioavailability. The use of P. ostreatus refuse holds promising potential for bioremediation purposes.

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“…Solid PAH was added prior to autoclaving. After several transfers, the enrichment cultures were plated on nutrient agar and sprayed with an acetone solution of PAH to produce a solid ¢lm of PAH on the plate; PAH-utilizing cultures were identi¢ed by the presence of a clear zone around the colony [17]. Metabolism was con¢rmed by means of a mineralization screening test [15].…”

Section: Organisms and Growth Conditionsmentioning

confidence: 99%

Utilization of mixtures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment

Dean-Ross

2002

FEMS Microbiology Ecology

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The ability of sediment bacteria to utilize polycyclic aromatic hydrocarbons (PAHs) when present as components of mixtures was investigated. One strain, identified as Mycobacterium flavescens, could utilize fluoranthene in the presence of pyrene, although utilization of pyrene was slower in the presence of fluoranthene than in its absence. The second strain, a Rhodococcus species, could utilize fluoranthene in the presence of anthracene, although the presence of fluoranthene slowed the rate of utilization of anthracene. Cometabolism of fluoranthene in these strains was confirmed by the isolation of metabolites of fluoranthene and by kinetic analysis of the rate of utilization of the growth substrate in the presence of fluoranthene. In both strains, metabolism of fluoranthene occurred on the fused ring of the fluoranthene molecule, producing 9-fluorenone-1-carboxylic acid. In the Rhodococcus sp., a second metabolite, a-(carboxymethylene)fluorene-1-carboxylic acid, was identified, indicating that this strain has the capacity to metabolize fluoranthene via ortho as well as meta cleavage. The presence of PAHs in a mixture produces interactive effects which can either increase or decrease the rate of utilization of individual PAHs, results which need to be taken into account when estimating rates of degradation in contaminated environments. ß

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Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates

Cited by 325 publications

References 16 publications

Species variation and plasmid incidence among fluorescent Pseudomonas strains isolated from agricultural and industrial soils

Species variation and plasmid incidence among fluorescent Pseudomonas strains isolated from agricultural and industrial soils

Bioaugmentation of Tar-Contaminated Soils Under Field Conditions Using Pleurotus Ostreatus Refuse From Commercial Mushroom Production

Utilization of mixtures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment

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