Abstract:Background
ABO genotyping has common tools for personal identification of forensic and transplantation field. We developed a new method based on a droplet allele‐specific PCR (droplet‐AS‐PCR) that enabled rapid PCR amplification. We attempted rapid ABO genotyping using crude DNA isolated from dried blood and buccal cells.
Methods
We designed allele‐specific primers for three SNPs (at nucleotides 261, 526, and 803) in exons 6 and 7 of the ABO gene. We pretreated dried blood and buccal cells with proteinase K, a… Show more
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