Rapid sampling microdialysis as a novel tool for parenchyma assessment during static cold storage and hypothermic machine perfusion in a translational ex vivo porcine kidney model

Hamaoui, Karim; Gowers, Sally A. N.; Damji, Samir; Rogers, Michelle; Leong, Chi Leng; Hanna, George B.; Darzi, Ara; Boutelle, Martyn G.; Papalois, Vassilios

doi:10.1016/j.jss.2015.07.004

Cited by 22 publications

(28 citation statements)

References 45 publications

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“…Current novel treatment methods tested in animal models are centred around ameliorating HMP-induced injuries of kidney grafts [51]. A minimally invasive method called rapid sampling microdialysis (rsMD), which was developed in a porcine HMP model [52], may help monitor tissue ATP levels and other metabolic markers during MP in real-time, promoting further investigations into the relationship of MP temperature and renal tissue ATP. Proteolytic enzymes like matrix metalloproteinases (MMPs) are known to be related to the pathophysiology of fibrotic renal diseases.…”

Section: The Effects Of Higher Perfusion Temperatures (>4-10 • C) On mentioning

confidence: 99%

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

Bellini

Yiu

Nozdrin

et al. 2019

JCM

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The recent advances in machine perfusion (MP) technology involve settings ranging between hypothermic, subnormothermic, and normothermic temperatures. Tissue level adenosine triphosphate (ATP) is a long-established marker of viability and functionality and is universal for all organs. In the midst of a growing number of complex clinical parameters for the quality assessment of graft prior to transplantation, a revisit of ATP may shed light on the underlying reconditioning mechanisms of different perfusion temperatures in the form of restoration of metabolic and energy status. This article aims to review and critically analyse animal and preclinical human studies (discarded grafts) during MP of three abdominal organs (liver, kidney, and pancreas) in which ATP was a primary endpoint. A selective review of recent novel reconditioning approaches relevant to mitigation of graft ischaemia-reperfusion injury via MP and for different perfusion temperatures was also conducted. With a current reiterated interest for oxygenation during MP, a re-introduction of tissue ATP levels may be valuable for graft viability assessment prior to transplantation. Further studies may help delineate the benefits of selective perfusion temperatures on organs viability.

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Section: The Effects Of Higher Perfusion Temperatures (>4-10 • C) On mentioning

confidence: 99%

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

Bellini

Yiu

Nozdrin

et al. 2019

JCM

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“…Tissue metabolism and biochemistry was assessed using a novel rapid sampling microdialysis system (rsMD) to sample and quantify levels of tissue lactate in the pancreas graft as previously described. 11 Lactate levels were analyzed in realtime (samples taken every 60 s) after the 24-h SCS and during 5 h of HMP and 2 h of NRP. rsMD assessment was undertaken in phase 2 porcine pancreas experiments but was not available during phase 3 involving unused human grafts; this was due to the rsMD apparatus not being available at the time of experimentation because of the acute and unplanned nature of the experiments (organs become available at any time, including weekends, once they are considered to be unsuitable for transplantation).…”

Section: Rapid Sampling Microdialysismentioning

confidence: 99%

Development of pancreatic machine perfusion: translational steps from porcine to human models

Hamaoui

Gowers

Sandhu

et al. 2018

Journal of Surgical Research

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“…Rogers et al 52 reported on the use of continuous online MD by using microfluidic sensors to study glucose, lactate, and potassium in cortical spreading depolarization. 53 Ideally, when commercially available and approved for human use, continuous MD might improve its temporal resolution and therefore will be able to detect metabolic changes that are short in duration and thus missed with conventional MD.…”

Section: Study Limitations and Future Directionsmentioning

confidence: 99%

Reappraisal of the reference levels for energy metabolites in the extracellular fluid of the human brain

Sánchez-Guerrero

Mur-Bonet

Vidal-Jorge

et al. 2016

J Cereb Blood Flow Metab

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Cerebral microdialysis is widely used in neurocritical care units. The goal of this study was to establish the reference interval for the interstitial fluid concentrations of energy metabolites and glycerol by using the extrapolation to zero-flow methodology in anesthetized patients and by constant perfusion at 0.3 µL/min in awake patients. A CMA-71 probe was implanted during surgery in normal white matter of patients with posterior fossa or supratentorial lesions, and the perfusion flow rate was randomized to 0.1, 0.3, 0.6, 1.2, and 2.4 µL/min. Within 24 h of surgery, perfusion was restarted at a constant 0.3 µL/min in fully awake patients. The actual interstitial fluid metabolite concentrations were calculated using the zero-flow methodology. In vitro experiments were also conducted to evaluate the reproducibility of the in vivo methodology. Nineteen patients (seven males) with a median age of 44 years (range: 21-69) were included in the in vivo study. The median (lower-upper) reference interval values were 1.57 (1.15-4.13 mmol/L) for glucose, 2.01 (1.30-5.31 mmol/L) for lactate, 80.0 (54.4-197.0 µmol/L) for pyruvate, and 49.9 (23.6-227.3 µmol/L) for glycerol. The reference intervals reported raises the need to reconsider traditional definitions of brain metabolic disturbances and emphasize the importance of using different thresholds for awake patients and patients under anesthesia.

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Rapid sampling microdialysis as a novel tool for parenchyma assessment during static cold storage and hypothermic machine perfusion in a translational ex vivo porcine kidney model

Cited by 22 publications

References 45 publications

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

Development of pancreatic machine perfusion: translational steps from porcine to human models

Reappraisal of the reference levels for energy metabolites in the extracellular fluid of the human brain

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