Rapid, robust method for measuring low concentrations of albumin in urine

Abstract

We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.

Circulating plasma vascular endothelial growth factor and microvascular complications of Type 1 diabetes mellitus: the influence of ACE inhibition

“…AER was measured on two overnight urine collections, performed every 6 months throughout the trial. Albumin was measured by the particle-enhanced immunoturbidimetric assay [24], and the mean of both collections was used in all analyses.…”

Amadori‐albumin correlates with microvascular complications and precedes nephropathy in type 1 diabetic patients

“…Urine albumin was measured by an immunoturbidimetric assay, and AER was assessed as the mean of two overnight urine collections [19].…”

Serum-free light-chain assay: clinical utility and limitations

“…Once purified, the antisera were digested with pepsin to produce F(ab) 2 fragments, these fragments were then adsorbed onto polystyrene latex particles to enhance stability and sensitivity (Figure 1). 3 This method provided an improved sensitivity for the determination of serum-free light-chain (sFLC) compared to previous methods. In addition to quantification, the method also allowed indirect determination of monoclonality by using the κ : λ ratio.…”