Rapid Regeneration of Dracocephalum Kotschyi Boiss. From Nodal Explants

Moradi, Kosar; Otroshy, Mahmoud

doi:10.5963/lsmr0301002

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…It has often been observed that BAP is more effective for shoot proliferation than other cytokinins. MS medium supplemented with 2 mg/l BAP gave better results for D. kotschyi shoot propagation than a medium with the same concentration of kinetin (Otroshy and Moradi 2013). Similar results were achieved for Mentha piperita and Scutellaria altissima (Grzegorczyk-Karolak et al 2017;Mehta et al 2012).…”

Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 59%

“…1c). Similarly, Otroshy and Moradi (2013) obtained the highest number of Dracocephalum kotshyi shoots (about seven shoots per explant) on medium contained 2 mg/l (about 8 µM) BAP. Medium with lower content, 1 mg/l (4 µM) BAP was significantly less effective, giving about five shoots/explant.…”

Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 81%

“…An efficient protocol for proliferation of D. forrestii was development and multiple shoots were obtained by culturing nodal explants on MS medium supplemented with 1 µM IAA and different type and concentrations of cytokinins: BAP, zeatin, kinetin or TDZ. There are many in vitro studies reported using nodal explants for the propagation of plants from the Lamiaceae family (Otroshy and Moradi 2013;Rani et al 2006;Ghandi et al 2004). The effect of the cytokinins on shoot proliferation, fresh and dry weight production was evaluated after 4 weeks of culture, as was the morphology of the D. forrestii shoots.…”

Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 99%

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Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

et al. 2018

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The current study estimates the effect of different cytokinins on shoot proliferation and biosynthesis of caffeic acid derivatives in Dracocephalum forrestii in vitro culture. The shoots were grown on Murashige and Skoog (MS) agar medium with 1 µM indole-3-acetic acid (IAA) and different content of 6-benzyloaminopurine (BAP), zeatin, kinetin (1, 2, 4, 8, 18 µM) or thidiazuron (TDZ) (0.1, 0.2, 0.5, 1, 2 µM). The highest multiplication rate (about seven shoots and/or buds per explant) was obtained after 4 weeks of culture on MS medium with 1 µM IAA and 8 or 16 µM BAP. Optimal biomass of plant material was also received on the same media. The identity of the compounds present in the hydromethanolic extracts from D. forrestii shoots grown on cytokinin-supplemented media was confirmed using UPLC-PDA-ESI-MS method. The analysis revealed the presence of nine metabolites recognized as caffeic acid derivatives. The content of the predominant phenolic acids in the extracts, i.e. rosmarinic acid (RA) and salvianolic acid B (SAB), was determined with UHPLC. The highest yield of RA was found in shoots cultivated in the medium containing 1 µM IAA and 2 µM BAP (18.7 mg/g DW). The highest level of SAB (5.3-5.9 mg/g DW) was identified in multiple shoots grown in the presence of 1 µM IAA and 0.5-1 µM TDZ or 2 µM BAP.

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Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 59%

Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 81%

Section: Effect Of Cytokinins On Shoot Proliferationmentioning

confidence: 99%

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Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

et al. 2018

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“…These results differed from the findings by Suraya et al [ 5 ], who stated that aseptic nodal segments of Phyllanthus niruri cultured on MS medium fortified with 1.0 mg/L BAP produced the highest number of shoots and the combination of kinetin and BAP induced multiple shoot formation in all nodal explants. MS medium supplemented with high BAP produced better results for Dracocephalum kotschyi shoot propagation [ 30 ]. Similar results were achieved for the bud proliferation of Musa acuminata [ 31 ], Mentha piperita [ 27 ] and Scutellaria altissima [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi)

Patuhai,

Wahab,

Yusoff

et al. 2023

Plants

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Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of A. paniculata. Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of A. paniculata was developed, and that elicitation enhanced the herbage yield and the production of andrographolide.

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