Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells

Abstract

Hence, one may speculate that the genetic alterations detected in these cell lines may have been present in the primary tumors from which they arose, and could reflect events that are relevant to the pathogenesis of BL and cervical carcinoma, respectively.Today, continuous suspension cultures are a common research tool; so common, in fact, that we seldom reflect on their origin or genetic constitution. Using the molecular cytogenetic techniques SKY, array CGH, and FISH, we have provided a complete and definitive karyotype of the original BL-derived cell line, Raji. Our results reveal a number of novel genomic aberrations, concurrent with the hallmark t(8;14) translocation. The use of continuous human cell lines has played a major role in the advancement of our understanding of hematopoietic and other malignancies. An appreciation of the genetic anomalies of a cancer cell line may aid in the correct interpretation of data generated in such a model. The current results may thus serve as a valuable reference for future studies on BL.

Prophylactic transfer of BCR-ABL–, PR1-, and WT1-reactive donor T cells after T cell–depleted allogeneic hematopoietic cell transplantation in patients with chronic myeloid leukemia

“…This increase in donor T-cell chimerism occurred 3 to 4 months earlier compared with a historical cohort of patients receiving T cell-depleted SCT with no planned adoptive T-cell transfer. 21 Therefore, we hypothesize that lymphodepletion induced by the conditioning regimen and the infusion of CD34 ϩ donor cells allowed for a rapid peripheral proliferation of transferred T-cell preparations without any competition against existing T-cell populations, and the lack of calcineurin inhibitors as pharmacologic immunosuppression did not jeopardize the proliferation of donor lymphocytes infused early after transplantation. Although the dose of ATG used in this trial is at the lower end of current dosing schedules, there might have been residual levels of ATG in the plasma of our patients potentially suppressing the activity of the first CTL dose on day 28.…”

“…Figure 2B summarizes the course of CD4 ϩ and CD8 ϩ donor chimerism in a historical control cohort that was transplanted with T cell-depleted grafts from matched sibling donors without receiving prophylactic DLI. 21 In these patients, the mean percentage of donor chimerism in T-cell subsets remained Ͻ 90% until 6 months after transplantation. The absolute count of lymphocyte subsets is shown in Figure 2C.…”

CD133 is a modifier of hematopoietic progenitor frequencies but is dispensable for the maintenance of mouse hematopoietic stem cells

“…11,12). Indeed, CD133 is widely used as a cell surface antigen to prospectively isolate human HSCs that can reconstitute hematopoiesis upon transplantation into mice (13,14), sheep (9), and humans (15). Besides HSCs derived from cord blood, bone marrow, and apheresis products (13,14,16), CD133 is detected on cancer cells from various malignant hematopoietic diseases, including acute and chronic myeloid and lymphoblastic leukemias (reviewed in ref.…”

Oxygen tension plays a critical role in the hematopoietic microenvironment in vitro

“…Subsequently, 2×10 5 fresh CD34 + cells were suspended in 0.1 mL of the medium and added to the upper chamber. The cells were allowed to migrate for 4 h at 37 °C.…”