Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction

Chen, Ding‐Ping; Wen, Yu‐Chuan; Lu, Jang‐Jih; Tseng, Ching‐Ping; Wang, Wei‐Ting

doi:10.1016/j.jfma.2018.06.014

Cited by 9 publications

(7 citation statements)

References 33 publications

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“…The SNaPshot method used in the present study offers a specific, sensitive, inexpensive and rapid alternative to the simultaneous screening for multiple mutations and subsequent confirmation by sequencing ( Quintans et al., 2004 ; Alina et al., 2005 ; Fondevila et al., 2017 ). This method has been extensively used in the blood group typing ( Palacajornsuk et al., 2009 ; Latini et al., 2014 ; Chen et al., 2019 ) and common mutations in genes related to some cancers ( Filippini et al., 2007 ; Hurst et al., 2009 ; Fariña Sarasqueta et al., 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Insecticide Resistance Mutations in Anopheles gambiae from Sierra Leone Using Multiplex SNaPshot and Sequencing

Yin

Yamba

Zheng

et al. 2021

Front. Cell. Infect. Microbiol.

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Vector control interventions including long-lasting insecticidal nets and indoor residual spraying are important for malaria control and elimination. And effectiveness of these interventions depends entirely on the high level of susceptibility of malaria vectors to insecticides. However, the insecticide resistance in majority of mosquito vector species across African countries is a serious threat to the success of vector control efforts with the extensive use of insecticides, while no data on insecticide resistance was reported from Sierra Leone in the past decade. In the present study, the polymerase chain reaction was applied for the identification of species of 757 dry adult female Anopheles gambiae mosquitoes reared from larvae collected from four districts in Sierra Leone during May and June 2018. And the mutations of kdr, rdl, ace-1 genes in An. gambiae were detected using SNaPshot and sequencing. As a result, one sample from Western Area Rural district belonged to Anopheles melas, and 748 An. gambiae were identified. Furthermore, the rdl mutations, kdr west mutations and ace-1 mutation were found. The overall frequency was 35.7%, 0.3%, 97.6% and 4.5% in A296G rdl, A296S rdl, kdrW and ace-1, respectively. The frequencies of A296G rdl mutation (P < 0.001), kdrW mutation (P = 0.001) and ace-1 mutation (P < 0.001) were unevenly distributed in four districts, respectively, while no statistical significance was found in A296S rdl mutation (P = 0.868). In addition, multiple resistance patterns were also found. In conclusion, multiple mutations involved in insecticide resistance in An. gambiae populations in Sierra Leone were detected in the kdrW, A296G rdl and ace-1 alleles in the present study. It is necessary to monitor vector susceptibility levels to insecticides used in this country, and update the insecticide resistance monitoring and management strategy.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Insecticide Resistance Mutations in Anopheles gambiae from Sierra Leone Using Multiplex SNaPshot and Sequencing

Yin

Yamba

Zheng

et al. 2021

Front. Cell. Infect. Microbiol.

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“…The genomic DNA of leukocytes was extracted following the method of DNA isolation kit (TIANGEN BIOTECH [Beijing] CO., LTD). Then, the preliminary genotype was detected with PCR‐SSP, and the specific primers were designed according to single nucleotide polymorphism 4 . The exons 6 and 7 of the ABO gene were amplified using PCR and their sequences were determined by direct sequencing, and the results were analyzed by comparing with reference sequence A101 (NG‐006669).…”

Section: Brief Methodsmentioning

confidence: 99%

Identification of a novel missense mutation (c.608A > G) in the ABO gene contributing to an A variant

XinMing

Huo

et al. 2020

Transfusion

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“…Scholars have recently advocated using RhD genetic testing for patients with a weak D phenotype to obtain accurate and actionable results for RhD blood typing and Rh Ig administration. [63][64][65] There is still a vast capacity for improving HDN's understanding and management in both developed and developing countries. Universal research-backed guidelines regarding RBC antigen matching transfusion techniques for women before pregnancy or during intrauterine transfusion would be a good place to start.…”

Section: Trends and Future Prospectsmentioning

confidence: 99%

Hemolytic Disease of the Newborn: A Review of Current Trends and Prospects

Myle¹,

Al-Khattabi

2021

PHMT

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Hemolytic disease of the newborn (HDN), also known as Erythroblastosis fetalis, is a hemolytic condition that predominantly affects rhesus-positive fetuses and infants born to rhesus-negative mothers. The pathophysiology of HDN begins with maternal antibodies attacking fetal red blood cells following alloimmunization due to rhesus or ABO incompatibility between the maternal and fetal blood. Previously, HDN was known to cause fetal death in 1% of all pregnancies, but with the advent of immunoprophylactic therapies, the condition can be currently fairly well managed with fewer complications if diagnosed early. Diagnosis calls for extensive history taking, physical examination, serological studies, and imaging modalities such as pelvic ultrasound scans. To prevent the disease, earlier intravenous immunoglobulin (IVIG) should be given to pregnant Rh-women who have not been sensitized. It is also vital to understand prospective complications such as severe hyperbilirubinemia and develop appropriate remedies. Because of its great incidence and nature, HDN has been thoroughly explored, and more studies are being conducted each year, revealing new insights about the condition. This review covers the disorder's etiology, diagnosis, and management, including the most current findings as of 2021, as well as trends and prospects, to help in future research and evidence-based medical practice.

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Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction

Cited by 9 publications

References 33 publications

Molecular Detection of Insecticide Resistance Mutations in Anopheles gambiae from Sierra Leone Using Multiplex SNaPshot and Sequencing

Molecular Detection of Insecticide Resistance Mutations in Anopheles gambiae from Sierra Leone Using Multiplex SNaPshot and Sequencing

Identification of a novel missense mutation (c.608A > G) in the ABO gene contributing to an A variant

Hemolytic Disease of the Newborn: A Review of Current Trends and Prospects

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