Rapid quantitative gas-chromatographic fractionation of the major urinary i7-oxosteroids and i7-oxogenic steroids

External quality assessment of urinary steroid profile analysis

“…Most participants used methyloxime/trimethylsilyl ether derivatives (MO TMS) followed by either a solvent or lipidex clean-up stage before analysis by GC or GCMS. One participant utilized 17-oxogenic methodology 3 before derivatization and GC analysis.…”

Male Pseudohermaphroditism Due to Partial End‐organ Insensitivity to Androgens

“…(1971). Urinary steroids were measured using gas chromatography as already described (Trafford & Makin, 1972). Plasma levels of testosterone, oestrogen and SHBG, and urinary steroid levels were measured both before and after a two-day course of dexamethasone (0.5 mg every 6 hours), to determine whether there was a significant adrenal contribution to the hormone profile.…”

Gas Chromatography of C19 and 17-Oxogenic Steroids in Urine

“…The identities of steroids by GC retention time was not always possible when separating steroid formate esters by packed column Gc. 16,17 Confirmation of the derivatives by mass spectrometry was not available at the time of these early studies. The electron impact mass spectra of clinically important androgens as their formate derivatives were not published until 1979.…”