Rapid, quantitative and ultra-sensitive detection of cancer biomarker by a SERRS-based lateral flow immunoassay using bovine serum albumin coated Au nanorods

Abstract

A rapid, sensitive, and stable SERRS-LFIA strip was developed for AFP detection using BSA-coated AuNRs as SERRS nanotags.

Nanogapped Fe3O4@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

“…To address this problem, we employed a salt-tolerant Au@BSA NP solution as a colorimetric marker to dilute the antibodies and evenly sprayed them on the NC membrane to build visible T lines for laser positioning. The Au@BSA NPs were fabricated according to our previously used method 16 and exhibited good dispersion and stability in a 0.2 mM NaCl solution (Figure S5). The detailed preparation method of 20 nm Au@BSA NPs can be found in Supporting Information S1.2.…”

“…As the most promising point-of-care testing (POCT) method, immunochromatographic assay (ICA) has attracted extensive attention because of its distinct advantages including portability, rapidness, low cost, and ability for multitarget analysis. − However, traditional ICA test strips employ a colloidal gold nanoparticle (AuNP) as the colorimetric signal reporter, which usually suffers from low sensitivity and quantitative ability and thus cannot meet the requirement of food safety screening. , In recent years, a novel surface-enhanced Raman scattering (SERS)-based ICA technique has been developed using SERS nanotags as superior optical reporters, which can generate ultrastrong (sensitive), specific (characteristic peak), and stable (no photobleaching) Raman signals for target quantification. − A variety of colloidal SERS tags (e.g., Au, Au@Ag, SiO 2 @Ag NPs) have been successfully applied in SERS-based ICA systems to achieve the sensitive detection of biomolecules (e.g., biomarkers, toxins, and immunoglobulins) in clinical samples or harmful ingredients (e.g., pesticides and mycotoxins) in food samples. − However, for microorganism detection, two major difficulties need to be solved via the SERS-ICA method. First, the size of foodborne pathogens is rather huge (0.5–3 μm) for the ICA system; as such, a NC membrane with big pores is required to provide good transport, thereby affecting the sensitivity and ability of multichannel detection.…”

Surface-Enhanced Raman Scattering-Based Lateral-Flow Immunoassay

“…Then, the required spectral range is selected, and a calibration curve is constructed from which important parameters, such as the detection limit and the working concentration range are determined. The approach, based on measuring and averaging the signal at several points within the T and C zones, has been successfully applied in many papers for cardio biomarker detection and quantification [ 56 ], HIV DNA detection [ 20 ], detection of neuron-specific enolase in blood plasma [ 21 ], detection of the β-adrenergic agonist brombuterol [ 42 ], and many other applications [ 25 , 35 , 39 , 40 , 41 , 43 , 44 , 46 , 47 , 55 , 63 , 64 , 65 ]. However, a number of questions arise regarding the implementation of this approach.…”

Metal Nanoparticles/MoS2 Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection

“…A wide range of diagnostic techniques have been proposed for the detection of serum AFP, such as polymerase chain reaction assay, immunoradiometric assay, magnetic resonance immunoassay, aptamer-based fluorescent assay, fluorometric immunoassays, − chemiluminescence assays, − electrochemical assays, − and metamaterial-assisted terahertz spectroscopy, among others. − However, most of these approaches present limited reliability and sensitivity for AFP or they are time-consuming and require the use of complex instruments. Thus, the development of alternative diagnostic techniques for the detection of AFP as a biomarker is still required.…”