Rapid quantitation of free fatty acids in human plasma by high-performance liquid chromatography

Mehta, Adosh; Oeser, Annette; Carlson, Marvin P.

doi:10.1016/s0378-4347(98)00403-4

Cited by 74 publications

(60 citation statements)

References 25 publications

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“…The compound has only ultraviolet (UV) end absorption; pre-column derivation was employed in combination with reversed-phase high-performance liquid chromatography (HPLC) (13)(14)(15).…”

Section: Quantification Of Bjomentioning

confidence: 99%

Preparation, Safety, Pharmacokinetics, and Pharmacodynamics of Liposomes Containing Brucea javanica Oil

Cui

Liu

et al. 2010

AAPS PharmSciTech

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Abstract. Brucea javanica oil-loaded liposomes (BJOL) were prepared through thin film hydration method and characterized by transmission electron microscope, dynamic light scattering, and differential scanning calorimetry. Acute toxicity of B. javanica oil (BJO) in liposomes was assessed by determining the number of deaths of Kunming mice over intravenous treatment for 2 weeks. The pharmacokinetic behavior of the main active component (oleic acid) was studied in SD rats. The pharmacodynamics of BJOL was investigated using MMC-7721 cell lines and mice with Lewis lung cancer. The commercial emulsion of BJO (BJOE) was used as a reference. The data showed that BJOL had an average diameter of 108.2 nm with a zeta potential of −57.0 mV, drug loading of 3.60%, and entrapment efficiency of 92.40%. The area under curve of BJO in liposomes and emulsions were 2.31 and 1.15 mg min/ml, respectively. Compared with BJOE, mean residence time and elimination half-time (t 1/2 ) increased 2.8-and 4.0-fold, respectively, and the clearance (CL) decreased 0.5-fold. In the acute toxicity test, the median lethal dose (LD 50 ) of BJOE was 7.35 g/kg. In contrast, all mice treated with liposomes survived even at the highest dosage (12.70 g/kg). The IC 50 value of BJOL group was one third of that of BJOE group (p<0.01), and a less weight loss was observed in the BJOL-treated animals (p<0.05). In conclusion, the present study suggests that BJOL significantly decreased toxicity of BJO and enhance the antitumor activity. Therefore, liposomes may be a potential effective delivery vehicle for this lipophilic antitumor drug.

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“…The compound has only ultraviolet (UV) end absorption; pre-column derivation was employed in combination with reversed-phase high-performance liquid chromatography (HPLC) (13)(14)(15).…”

Section: Quantification Of Bjomentioning

confidence: 99%

Preparation, Safety, Pharmacokinetics, and Pharmacodynamics of Liposomes Containing Brucea javanica Oil

Cui

Liu

et al. 2010

AAPS PharmSciTech

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“…These include thin-layer, 12 gas-liquid, 37 and high-performance liquid chromatography (HPLC) 23,29,36,38,40 applied to…”

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confidence: 99%

Detection of Nonesterified (Free) Fatty Acids in Bovine Serum: Comparative Evaluation of Two Methods

2004

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Abstract. A method comparison study for the determination and quantitation of nonesterified fatty acids (NEFAs) in serum, using the commercial ''NEFA C'' enzymatic test kit, was performed using the spectrophotometric method recommended by the manufacturer and a modified procedure optimized for the use of a microplate reader, a 96-well microtiter plate, and small sample volumes (10 l). Linearity, sensitivity, and precision using the test kit were determined for each method of detection. The assay was linear from 0 to 1.97 mEq/liter for both procedures, and the limits of detection were determined to be 0.22 (Ϯ0.074) and 0.05 (Ϯ0.002) mEq/liter for the spectrophotometer and microplate reader, respectively. Pairs of measurements for bovine serum samples were compared and evaluated by a mean difference plot method and not regression analysis, a method that has been shown to be inappropriate for method comparison studies. The difference plot was used to evaluate the systematic bias between the 2 methods. Random error is reported on the basis of SD differences, and ''limits of agreement'' are used to describe the maximum differences likely to occur between the 2 methods. Results suggest that the microplate reader method can be used reliably in place of the recommended spectrophotometric method. The microplate reader method is preferred because of its high throughput capabilities, simultaneous analysis of all the standards and samples, use of small sample and reagent volumes, and reduction in labor requirements and costs.The measurement of nonesterified fatty acids (NEFAs) in dairy cows can be used to assess dry cow nutritional management and, in conjunction with cholesterol determination, serves as an indictor of postpartum disease risk. 6,11,13,20 Increased energy demand during late gestation and early lactation, combined with a decline in dry matter intake before parturition, make dairy cows uniquely susceptible to negative energy balance (NEB). Plasma NEFA concentrations can be used as a measure of adequate energy intake, with increased concentrations associated with a NEB. Several studies have shown a positive association between increased plasma NEFA concentrations during the last 2-3 weeks of gestation and the occurrence of postpartum diseases such as ketosis, fatty liver, displaced abomasums, retained placentas, and metritis. 6,11,13,20 Feeding strategies designed to increase energy intake during this period are desirable for optimal postpartum health and performance. Thus, monitoring of plasma NEFA is a useful tool for dry cow management.A large number of methods for detection and isolation of NEFA have been previously reported. These include thin-layer, 12 gas-liquid, 37 and high-performance liquid chromatography (HPLC) 23,29,36,38,40 applied to

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“…In the experiment, the samples were firstly separated from protein by trichloroacetic acid and then extracted with n-hexane three times. The derivatization procedure was referenced in the report by Mehta et al (1998). The details were not described here.…”

Section: Fatty Acid Extraction and Derivatizationmentioning

confidence: 99%

Discrimination of type 2 diabetes mellitus corresponding to different traditional Chinese medicine syndromes based on plasma fatty acid profiles and chemometric methods

Zhang

Huang

et al. 2012

Journal of Ethnopharmacology

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Rapid quantitation of free fatty acids in human plasma by high-performance liquid chromatography

Cited by 74 publications

References 25 publications

Preparation, Safety, Pharmacokinetics, and Pharmacodynamics of Liposomes Containing Brucea javanica Oil

Preparation, Safety, Pharmacokinetics, and Pharmacodynamics of Liposomes Containing Brucea javanica Oil

Detection of Nonesterified (Free) Fatty Acids in Bovine Serum: Comparative Evaluation of Two Methods

Discrimination of type 2 diabetes mellitus corresponding to different traditional Chinese medicine syndromes based on plasma fatty acid profiles and chemometric methods

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