Rapid qualitative profiling and quantitative analysis of phenolics in Ribes meyeri leaves and their antioxidant and antidiabetic activities by HPLC‐QTOF‐MS/MS and UHPLC‐MS/MS

Abstract

Ribes meyeri leaves are used as traditional Kazakh medicine in China. However, no study on the characterization of the phenolic compounds in R. meyeri leaves has been reported, resulting in the lack of quality control measures and poor standardization. This study was conducted to identify the phenolic compounds in R. meyeri leaves and evaluate their antioxidant and antidiabetic activities. A total of 77 phenolics were tentatively identified by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. Ultra‐high performance liquid chromatography coupled with triple quadrupole mass spectrometry was applied to simultaneously quantify 12 phenolics in R. meyeri leaves. Rutin, epigallocatechin, isoquercitrin, epicatechin, protocatechuic acid, and kaempferol‐3‐O‐rutinoside were abundant in the R. meyeri leaves. The methanol extract and four different extracts enhanced the glucose uptake in 3T3‐L1 adipocytes. The ethyl acetate extracts showed a total phenolic content of 966.89 ± 3.59 mg gallic acid equivalents/g, a total flavonoid content of 263.58 ± 17.09 mg catechin equivalents/g, and good protein‐tyrosine phosphatase‐1B inhibitory activities (IC50: 0.60 ± 0.03 μg/mL). To our knowledge, this work is the first to identify and quantify the major phenolics in R. meyeri leaves.

Polyphenolic composition and antioxidant potential of underutilized Himalayan wild edible berries by high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry

“…The results were in accordance with previous literature [32, 38]. In the case of apigenin and luteolin derivatives, their identification was carried out by comparison of RT, UV spectra, and MS/MS spectra (peaks 30 and 80 ) [29].…”

“…The vanillic acid was observed in all four samples whereas gallic acid was detected only in P.cerasoides and D. indica . The peak 13 showed the molecular ion peak at m/z 315.4 [M–H] – and had fragment ion at m/z 152.3 due to the elimination of hexose moiety and was tentatively assigned as dihydroxybenzoic‐4‐ O ‐hexoside [29]. Similarly, Peaks 10, 21, and 53 produced molecular ion peaks [M–H] – at m/z 299.3 and MS 2 product ion at m/z 137.4, due to the loss of hexose moiety.…”

“…Therefore, on the basis of available reports, elution sequence, and fragmentation pattern, peaks 36 and 37 were assigned as coumaroyl quinic acids I and II, respectively (Figure S3). Likewise, peaks 17, 29, 33, and 38 were identified on the basis of literature and fragmentation behavior as hydroxyferulic acid hexoside, dihydrocaffeic acid hexoside I, dihydrocaffeic acid hexoside II [33, 34], and p ‐coumaroyl‐ O ‐hexoside [29], respectively. The HPLC chromatogram (Figure 3) displayed peaks 42 and 45 in a significant amount, at m/z 706.7 [M‐H] – and exhibited fragment ion peaks at m/z 353.2 [M–H‐353] – , m/z 336.2 [M–H‐353‐H 2 O] – , and m/z 191.3 [M–H‐353‐162] – leading to the identification of dimer of caffeoylquinic acid which in line with previously reported berberis species, that is, from the leaves of B. thunbergii [35].…”

Chemical Profiling of Kaliziri Injection and Quantification of Six Caffeoyl Quinic Acids in Beagle Plasma by LC-MS/MS

“…Additionally, the plasma quantification of key components has not been studied. Tandem mass spectrometry can powerfully characterize chemical components in herbs and plants [18][19][20][21][22]. Ultra-high-performance liquid chromatography coupled with quadrupole orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS) hybridizes the high mass resolution of orbitrap and the excellent selectivity of the quadrupole [23].…”

A Three-Reagent “Green” Paper-Based Analytical Device for Solid-Phase Spectrometric and Colorimetric Determination of Dihydroquercetin

“…Antioxidants, such as flavonoids, are among them. To date, a number of analytical methods have been developed for the determination of flavonoids, such as HPLC [13][14][15][16][17][18], gas chromatography [19,20], capillary electrophoresis [21][22][23], voltammetry [24][25][26], and spectrophotometry [27][28][29][30][31].…”