Rapid purification of lactate dehydrogenase from rat liver and hepatoma: A new approach

Purification and properties of calf thymus polyadenosine diphosphate ribose polymerase

“…The presence of the poly(A)DPR in vivo was demonstrated [24]. The existence of the enzyme in rat liver nuclei was reported [5,6].…”

“…Sephadex G-150, Sepharose 4B and blue dextran were purchased from Pharmacia Fine Chemicals. Blue dextran-Sepharose (available from Pharmacia) was prepared according to [24]. Solubilized chromatin…”

Glucan-binding Proteins of Streptococcus mutatis Serotype c

“…(iii) Dextran 2000-Sepharose, consisting of dextran (molecular weight 2000000; Pharmacia) coupled to epoxy-activated Sepharose. (iv) Blue Dextran-Sepharose, made by coupling Blue Dextran 2000 (Pharmacia) to cyanogen bromide-act iva ted Sepharose (Pharmacia) by the procedure of Ryan & Vestling (1974). This column was used in conjunction with one containing Cibacron Blue F3GA coupled to Sepharose (Heyns & de Moor, 1974) in order to remove proteins with affinity for the dye.…”

Purification and Properties of Two Different Dihydroxyacetone Reductases inGluconobacter suboxydansGrown on Glycerol

“…Hydroxyapatite was prepared by the method described by Tiselius et al 29) Blue-Dextran (BD)-Sepharose 4B was prepared by method of Ryan and Vestling. 30) Microorganism and growth conditions. G. suboxydans IFO 3255 was used.…”