Rapid purification and characterization of l-dopachrome-methyl ester tautomerase (macrophage-migration-inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi

Pennock, Joanne L.; Behnke, Jerzy M.; Bickle, Q. D.; Devaney, Eileen; Grencis, Richard K.; Isaac, R. Elwyn; Joshua, G. W. P.; Selkirk, Murray E.; Zhang, Y.; Meyer, David J.

doi:10.1042/bj3350495

Cited by 44 publications

(41 citation statements)

References 16 publications

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“…In another experiment performed with T. britovi (15000 larvae) on pigs, CPK, AST and ALT presented increased activity during the infection (Oltean et al, 2009). The irrelevant results obtained in the phagocytic assay could be explained through the results obtained by Pennock et al (1998), Tan et al (2001) and Freitas and Pearce (2010), who demonstrated the presence of a macrophage migration-inhibitory factor (MIF) in T. spiralis larvae, very similar to a mammalian cytokine, which might contribute to subversion of host defences. The mammalian MIF is inhibitory for monocyte-macrophage migration, towards glucocorticoid activity (Calandra et al, 1995), immunoglobulin synthesis (Tomura et al, 1999) and natural killer-cell activity (Apte et al, 1998), but not all this properties have been demonstrated yet in MIF produced by T. spiralis.…”

Section: Discussionmentioning

confidence: 84%

Characterization of host-parasite interactions during the experimental Trichinella spiralis infection in pigs

et al. 2012

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The aim of the present experiment was to assess the clinical, haematological, biochemical and immunological responses of pigs experimentally infected with Trichinella spiralis. One group of 6 pigs was infected with 1500 larvae/kg body weight and one group of 7, was maintained as control. The highest larval density was in the diaphragm (504.3 ± 8.2). During the experimental T. spiralis infection in pigs, increased values of total granulocyte number, eosinophils, blood glucose, K+ concentration, CK, ALT, AST and ALP were registered in different stages of infection. A slight increase of the phagocytic activity was registered 14 days p.i., in the infected group. Significantly increased values in lymphocyte activity, in infected group, was observed 30 and 60 days p.i. comparative with control. The specific IgG antibodies were detectable by ELISA at 14 days p.i. The results obtained in this study provide a better understanding about complete interactions occurring during Trichinella infection in pigs.

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Section: Discussionmentioning

confidence: 84%

Characterization of host-parasite interactions during the experimental Trichinella spiralis infection in pigs

et al. 2012

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“…In other studies on parasites, the existence of host cytokine-like molecules, such as homologs of migration inhibition factor in Burgia malayi (Pastrana et al 1998;Pennock et al 1998), IFN-c-like protein in Trichuris muris (Grencis and Entwistle 1997) and IL-4-like molecules in Anisakis simplex (Cuellar et al 2001) have been reported. In western blot analysis, however, we could not find generally recognized sizes of molecules.…”

Section: Discussionmentioning

confidence: 95%

Expression of TGF-?-like molecules in the life cycle of Schistosoma japonicum

Hirata

Hara

et al. 2005

Parasitol Res

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The transforming growth factor beta (TGF-beta) family controls an extremely wide range of biological activities, such as the growth and differentiation of cells, and immunological events against infectious agents. Although TGF-beta homologs appear to be widely present in metazoan animals, studies of parasite-derived molecules are relatively few. Using antibodies against anti-mouse TGF-beta1, -beta2, and -beta3, we show the expression of TGF-beta-like molecules in Schistosoma japonicum cercariae, schistosomula, eggs and adult worms. Intense immunoreactivity was found on the surface of free-living cercarial bodies. In transverse sections of cercariae, the molecules were localized in the tegument and subtegumental cells, and the number and distribution of producing cells significantly differed with each antibody. In the skin-migrating stage, the expression in the tegumental surface gradually decreased and became almost negative within 48 h of exposure. In adult worms and eggs, the reactivity was found in subtegumental cells and in cells of a tubular structure, respectively. In western blot analysis, the detection of conventional TGF-beta molecules failed. The expression of TGF-beta-like molecules was distinctly regulated at each developmental stage.

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“…Our data strongly suggest that macrophages provide a crucial link between parasitic infections and eosinophil chemotaxis. Because the production of MIF-like enzymatic activity has been demonstrated in a variety of nematode parasites (35), Ym1 induction by parasite-secreted MIF and subsequent eosinophil recruitment to the tissues could be a widely spread mechanism accounting at least partially for the well-known phenomenon of tissue eosinophilia in parasitic infections. Although each of these events has been demonstrated in isolation, we have not yet shown a direct link between BmMIF, YM1 induction, and recruitment of eosinophils.…”

Section: Discussionmentioning

confidence: 99%

ABrugia malayiHomolog of Macrophage Migration Inhibitory Factor Reveals an Important Link Between Macrophages and Eosinophil Recruitment During Nematode Infection

Falcone

Loke

Zang

et al. 2001

The Journal of Immunology

118

114

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Infections with the helminth parasite Brugia malayi share many key features with Th2-mediated allergic diseases, including recruitment of eosinophils. We have investigated the dynamics of inflammatory cell recruitment under type 2 cytokine conditions in mice infected with B. malayi. Among the cells recruited to the site of infection is a novel population of “alternatively activated” macrophages that ablate cell proliferation and enhance Th2 differentiation. By profiling gene expression in this macrophage population, we found a dramatic up-regulation of a recently described eosinophil chemotactic factor, eosinophil chemotactic factor-L/Ym1, representing over 9% of clones randomly selected from a cDNA library. Because B. malayi is known to secrete homologs (Bm macrophage migration inhibitory factor (MIF)-1 and -2) of the human cytokine MIF, we chose to investigate the role this cytokine mimic may play in the development of the novel macrophage phenotype observed during infection. Strikingly, administration of soluble recombinant Bm-MIF-1 was able to reproduce the effects of live parasites, leading both to the up-regulation of Ym1 by macrophages and a marked recruitment of eosinophils in vivo. Because activity of Bm-MIF-1 is dependent upon an amino-terminal proline, this residue was mutated to glycine; the resultant recombinant (Bm-MIF-1G) was unable to induce Ym1 transcription in macrophages or to mediate the recruitment of eosinophils. These data suggest that macrophages may provide a crucial link between helminth parasites, their active cytokine mimics, and the recruitment of eosinophils in infection.

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Rapid purification and characterization of l-dopachrome-methyl ester tautomerase (macrophage-migration-inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi

Cited by 44 publications

References 16 publications

Characterization of host-parasite interactions during the experimental Trichinella spiralis infection in pigs

Characterization of host-parasite interactions during the experimental Trichinella spiralis infection in pigs

Expression of TGF-?-like molecules in the life cycle of Schistosoma japonicum

ABrugia malayiHomolog of Macrophage Migration Inhibitory Factor Reveals an Important Link Between Macrophages and Eosinophil Recruitment During Nematode Infection

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