Rapid prototyping fabrication of soft and oriented polyester scaffolds for axonal guidance

Kaplan, Ben; Merdler, Uri; Szklanny, Ariel A.; Redenski, Idan; Guo, Shengming; Bar-Mucha, Zemach; Michael, Noah; Levenberg, Shulamit

doi:10.1016/j.biomaterials.2020.120062

Cited by 39 publications

(35 citation statements)

References 47 publications

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“…Furthermore, recently, a unique fabrication technique based on 3D printing was developed to generate highly oriented and anatomically personalized PLLA/PLGA scaffolds to match the aligned topography of spinal cord tissues. The oriented PLLA/PLGA scaffolds guided regenerating axons to linear conformations and supported the growth and guidance of iPSC-derived neurons in the complete spinal cord transection model [96].…”

Section: Pla/plga Scaffoldsmentioning

confidence: 88%

“…PLGA can be combined with poly-L-lactic acid (PLLA) to form the PLLA/PLGA scaffolds, which serve as biocompatible and biodegradable matrices for cell attachment, proliferation, differentiation and organization in muscle, bone and spinal cord tissue engineering [34,64,[94][95][96]. Using the salt-leaching technique, highly porous PLLA/PLGA scaffolds can be fabricated with pore sizes of 212-600 µm and 93% porosity [34,64,[94][95][96].…”

Section: Pla/plga Scaffoldsmentioning

confidence: 99%

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Spinal Cord Repair: From Cells and Tissue Engineering to Extracellular Vesicles

Guo

Redenski

Levenberg

2021

Cells

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Spinal cord injury (SCI) is a debilitating condition, often leading to severe motor, sensory, or autonomic nervous dysfunction. As the holy grail of regenerative medicine, promoting spinal cord tissue regeneration and functional recovery are the fundamental goals. Yet, effective regeneration of injured spinal cord tissues and promotion of functional recovery remain unmet clinical challenges, largely due to the complex pathophysiology of the condition. The transplantation of various cells, either alone or in combination with three-dimensional matrices, has been intensively investigated in preclinical SCI models and clinical trials, holding translational promise. More recently, a new paradigm shift has emerged from cell therapy towards extracellular vesicles as an exciting “cell-free” therapeutic modality. The current review recapitulates recent advances, challenges, and future perspectives of cell-based spinal cord tissue engineering and regeneration strategies.

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Section: Pla/plga Scaffoldsmentioning

confidence: 88%

Section: Pla/plga Scaffoldsmentioning

confidence: 99%

Spinal Cord Repair: From Cells and Tissue Engineering to Extracellular Vesicles

Guo

Redenski

Levenberg

2021

Cells

Self Cite

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“…However, most studies only focused on topology effect on cell growth or tissue regeneration with single micro-sized topologies or nano-sized topologies, whereas few simultaneously considered the both aspects. Considering the naturally physiological structure of peripheral nerve with strip-like shape, an aligned microtopography on scaffolds is anticipated to be beneficial for nerve regeneration (20). As an aliphatic polyester, PCL with good biocompatible and biodegradable properties has been approved by Food and Drug Administration for biomedical application in clinic (21).…”

Section: Discussionmentioning

confidence: 99%

Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

Zheng

et al. 2021

Sci. Adv.

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Anisotropic topographies and biological cues can simulate the regenerative microenvironment of nerve from physical and biological aspects, which show promising application in nerve regeneration. However, their synergetic influence on injured peripheral nerve is rarely reported. In the present study, we constructed a bionic microenvironment-inspired scaffold integrated with both anisotropic micro-nanocomposite topographies and IKVAV peptide. The results showed that both the topographies and peptide displayed good stability. The scaffolds could effectively induce the orientation growth of Schwann cells and up-regulate the genes and proteins relevant to myelination. Last, three signal pathways including the Wnt/β-catenin pathway, the extracellular signal–regulated kinase/mitogen-activated protein pathway, and the transforming growth factor–β pathway were put forward, revealing the main path of synergistic effects of anisotropic micro-nanocomposite topographies and biological cues on neuroregeneration. The present study may supply an important strategy for developing functional of artificial nerve implants.

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“…Rapid regeneration of axons along the orientation of scaffolds is key for repairing long-distance nerve defects [45].…”

Section: Drg Behaviormentioning

confidence: 99%

Pure-silk fibroin hydrogel with stable aligned micropattern toward peripheral nerve regeneration

Chen

Tang

et al. 2021

Nanotechnology Reviews

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Successful repair of long-distance peripheral nerve injuries remains a challenge in the clinic. Rapid axon growth is a key to accelerate nerve regeneration. Herein, a pure silk fibroin (SF) hydrogel with a combination of high-strength and aligned microgrooved topographic structure is reported. The hydrogels exhibit excellent mechanical properties with high strength. Good biocompatibility also allows the hydrogels to support cell survival. Significantly, the hydrogel with aligned microgrooved structures enables the aligned growth of Schwann cells. Moreover, the hydrogel holds a strong capacity for promoting axon growth and guiding neurite sprouting. Thus, this micropatterned SF hydrogel would have great potential for peripheral nerve regeneration.

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Rapid prototyping fabrication of soft and oriented polyester scaffolds for axonal guidance

Cited by 39 publications

References 47 publications

Spinal Cord Repair: From Cells and Tissue Engineering to Extracellular Vesicles

Spinal Cord Repair: From Cells and Tissue Engineering to Extracellular Vesicles

Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

Pure-silk fibroin hydrogel with stable aligned micropattern toward peripheral nerve regeneration

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