Rapid profiling strategy for oligosaccharides and polysaccharides by MALDI TOF mass spectrometry

Wang, Junqiao; Zhao, Jing; Nie, Shaoping; Xie, Mingyong; Li, Shaoping

doi:10.1016/j.foodhyd.2021.107237

Cited by 17 publications

(11 citation statements)

References 36 publications

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“…This might be because of potassium ions were better able to promote the ionization of saccharides than sodium ions, which was related to the distribution and contents of potassium ions and sodium ions in A. lappa . Additionally, the peak intensities of these saccharides decreased with the increasing of molecular weight ( m / z ) ( Figure 3 a and Figure S4 ), which might be because the high-molecular-weight hindered an effective ionization in mass spectrum analysis without any separation or purification process [ 21 ]. Therefore, we speculated that the molecular weight of A. lappa saccharides could be larger than the present results (DP10) because the section was thin enough (not enough saccharide contents) to limit the ionization of the polysaccharide.…”

Section: Resultsmentioning

confidence: 99%

“…Firstly, nine representative compounds were prepared, including L-arginine (2 mM, dissolved in ultrapure water), proline (2 mM, dissolved in ultrapure water), phenylalanine (2 mM, dissolved in ultrapure water), caffeic acid (6 mM, dissolved in MeOH), quercetin (6 mM, dissolved in MeOH), chlorogenic acid (6 mM, dissolved in MeOH), ursolic acid (6 mM, dissolved in MeOH), and 1,5-dicaffeoylquinic acid (6 mM, dissolved in MeOH), 1,3,5-tricaffeoylquinic acid (6 mM, dissolved in MeOH). The matrix preparation process was based on previous studies with moderate modification [ 21 , 22 ]. Specifically, DHB, CHCA, SA, DHAP, 9-AA and NEDC were prepared in 0.1% TFA buffer (ACN/water, 1:1, v / v ) at 10 mg/mL, respectively, as MALDI matrices.…”

Section: Methodsmentioning

confidence: 99%

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Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Qiu

Jiang

et al. 2022

Foods

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This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in Arctium lappa L. (A. lappa) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in A. lappa roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in A. lappa roots, which would promote the processing technologies for A. lappa roots and help us to understand the plant metabolism process.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Qiu

Jiang

et al. 2022

Foods

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“…Advanced sequencing methods combine sequencing experiments of fractionated polysaccharides and oligosaccharides that are treated with hydrolyzed enzymes to generate smaller fractions and the database search could obtain the structure of the novel prebiotics obtained from microalgae ( 115 ). Also, the recent development of Multiple Reaction Monitoring mass spectrometry (MRM–MS) has made significant progress in the assessment and quantification of biofuel macromolecules.…”

Section: Methods For Identification Of Microalgae Prebiotics Macromol...mentioning

confidence: 99%

Microalgae Bioactive Carbohydrates as a Novel Sustainable and Eco-Friendly Source of Prebiotics: Emerging Health Functionality and Recent Technologies for Extraction and Detection

et al. 2022

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There is a global interest in the novel consumption, nutritional trends, and the market of new prebiotic sources and their potential functional impacts. Commercially available nutritional supplements based on microalgae that are approved to be edible by FDA, like Arthrospira platensis (Cyanobacteria) and Chlorella vulgaris (Chlorophyta) become widely attractive. Microalgae are rich in carbohydrates, proteins, and polyunsaturated fatty acids that have high bioactivity. Recently, scientists are studying the microalgae polysaccharides (PS) or their derivatives (as dietary fibers) for their potential action as a novel prebiotic source for functional foods. Besides, the microalgae prebiotic polysaccharides are used for medication due to their antioxidant, anticancer, and antihypertensive bioactivities. This review provides an overview of microalgae prebiotics and other macromolecules’ health benefits. The phytochemistry of various species as alternative future sources of novel polysaccharides were mentioned. The application as well as the production constraints and multidisciplinary approaches for evaluating microalgae phytochemistry were discussed. Additionally, the association between this potential of combining techniques like spectroscopic, chromatographic, and electrochemical analyses for microalgae sensation and analysis novelty compared to the chemical methods was emphasized.

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“…Since their discovery, both ESI and MALDI are the commonly used ionization methods for MS-based proteomic analysis, and the resulting data suggests a great deal of complementarity between ESI-MS and MALDI-MS [ 5 ]. MALDI is a “soft ionization” technique that produces rapid and effective ionization of a wide range of biomolecules (amino acids [ 6 ], peptides [ 5 ], proteins [ 7 ], oligonucleotides [ 8 ], oligosaccharides [ 9 ], and other organic molecules, such as small drugs [ 10 ]/metabolites [ 11 ] or large synthetic polymers [ 12 ]) using a matrix to absorb laser energy to produce ions with minimal fragmentation. The distinction between MALDI and ESI ionization sources is that the former produces low charged ions (singly and doubly protonated ions), which allows for easy molecular mass determination for most biomolecules [ 13 ] and easy interpretation of data comparative to ESI-MS [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research

et al. 2022

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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is one of the most widely used techniques in proteomics to achieve structural identification and characterization of proteins and peptides, including their variety of proteoforms due to post-translational modifications (PTMs) or protein–protein interactions (PPIs). MALDI-MS and MALDI tandem mass spectrometry (MS/MS) have been developed as analytical techniques to study small and large molecules, offering picomole to femtomole sensitivity and enabling the direct analysis of biological samples, such as biofluids, solid tissues, tissue/cell homogenates, and cell culture lysates, with a minimized procedure of sample preparation. In the last decades, structural identification of peptides and proteins achieved by MALDI-MS/MS helped researchers and clinicians to decipher molecular function, biological process, cellular component, and related pathways of the gene products as well as their involvement in pathogenesis of diseases. In this review, we highlight the applications of MALDI ionization source and tandem approaches for MS for analyzing biomedical relevant peptides and proteins. Furthermore, one of the most relevant applications of MALDI-MS/MS is to provide “molecular pictures”, which offer in situ information about molecular weight proteins without labeling of potential targets. Histology-directed MALDI-mass spectrometry imaging (MSI) uses MALDI-ToF/ToF or other MALDI tandem mass spectrometers for accurate sequence analysis of peptide biomarkers and biological active compounds directly in tissues, to assure complementary and essential spatial data compared with those obtained by LC-ESI-MS/MS technique.

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Rapid profiling strategy for oligosaccharides and polysaccharides by MALDI TOF mass spectrometry

Cited by 17 publications

References 36 publications

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

Microalgae Bioactive Carbohydrates as a Novel Sustainable and Eco-Friendly Source of Prebiotics: Emerging Health Functionality and Recent Technologies for Extraction and Detection

Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research

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