Rapid profiling of alkaloid analogues in Sinomenii Caulis by an integrated characterization strategy and quantitative analysis

Jiang, Zheng‐Meng; wang, Lan-Jin; Pang, Haijun; Yong, Guo; Xiao, Ping-Ting; Chu, Chu; Guo, Long; Liu, E‐Hu

doi:10.1016/j.jpba.2019.06.011

Cited by 33 publications

(20 citation statements)

References 31 publications

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“…These alkaloids are recommended for improving the quality control of S. acutum stem in pharmacopeias and for use as relevant standards in the future. The use of sinomenine and magnoflorine as chemical markers was also consistent with the published literature conclusions 2 , 33 . The other three alkaloids proposed together with sinomenine and magnoflorine better reflect the overall quality of this herb.…”

Section: Discussionsupporting

confidence: 89%

“…A multicomponent detection method should be used when a single component cannot reflect the medicinal materials' overall activity 23 . The pharmacological activities of sinomenine 2 , 24 – 26 , magnoflorine 14 , 27 , coclaurine 28 , acutumine 29 , and higenamine 30 , 31 well represent the main indications or bioactivities of S. acutum stem, which are anti-inflammation, analgesia, anti-hypertension, anti-arrhythmia, anticancer, and immunomodulation 32 . Therefore, based on the multivariate analysis results and the bioactivities of these components, it was reasonable and representative to identify these specific alkaloids, i.e., sinomenine, magnoflorine, higenamine, acutumine, and coclaurine, as chemical markers of S. acutum stem.…”

Section: Discussionmentioning

confidence: 99%

“…Alkaloids, dominated by sinomenine, are the principal chemical components of S. acutum stem. Pharmacological research and clinical practice have proven the excellent anti-inflammatory and analgesic effects of sinomenine 1 , which is commonly manufactured as a finished product to treat joint diseases and sports injuries 2 . Therefore, sinomenine has been considered a quality marker for this herb in the Chinese Pharmacopoeia, Japanese Pharmacopoeia, Korean Pharmacopoeia, and European Pharmacopoeia 3 – 6 .…”

Section: Introductionmentioning

confidence: 99%

“…Phytochemical studies have demonstrated that nearly one hundred alkaloids are present in this herb. It possesses types of morphinans, aporphines, protoberberines, benzylisoquinolines and other compounds, such as sinomenine, magnoflorine, coclaurine, acutumine, and higenamine 2 , 7 . In addition, the safety of the alkaloids in S. acutum stem, especially higenamine and coclaurine, should be considered.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of chemical markers for improving the quality and safety control of Sinomenium acutum stem by the simultaneous determination of multiple alkaloids using UHPLC-QQQ-MS/MS

Huang

Wang

et al. 2020

Sci Rep

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Sinomenium acutum stem is a popular traditional Chinese medicine used to treat bone and joint diseases. Sinomenine is considered the only chemical marker for the quality control of S. acutum stem in mainstream pharmacopeias. However, higenamine in S. acutum stem is a novel stimulant that was banned by the World Anti-Doping Agency in 2017. Therefore, enhancing the quality and safety control of S. acutum stem to avoid potential safety risks is of utmost importance. In this study, a fast, sensitive, precise, and accurate method for the simultaneous determination of 11 alkaloids in S. acutum stem by ultrahigh-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS) was established. This method successfully analyzed thirty-five batches of S. acutum stem samples. The average contents of sinomenine, magnoflorine, coclaurine, acutumine, higenamine, sinoacutine, palmatine, magnocurarine, columbamine, 8-oxypalmatine, and jatrorrhizine were 24.9 mg/g, 6.35 mg/g, 435 μg/g, 435 μg/g, 288 μg/g, 44.4 μg/g, 22.5 μg/g, 21.1 μg/g, 15.8 μg/g, 9.30 μg/g, and 8.75 μg/g, respectively. Multivariate analysis, including principal component analysis (PCA), orthogonal partial least square method-discriminant analysis (OPLS-DA), and hierarchical cluster analysis (HCA), were performed to characterize the importance and differences among these alkaloids in S. acutum stem samples. As a result, sinomenine, magnoflorine, coclaurine, acutumine, and higenamine are proposed as chemical markers for quality control. Higenamine and coclaurine are also recommended as chemical markers for safety control. This report provides five alkaloids that can be used as chemical markers for improving the quality and safety control of S. acutum stem. It also alerts athletes to avoid the risks associated with consuming S. acutum stem.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Discovery of chemical markers for improving the quality and safety control of Sinomenium acutum stem by the simultaneous determination of multiple alkaloids using UHPLC-QQQ-MS/MS

Huang

Wang

et al. 2020

Sci Rep

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“…The in vitro chemical composition of THSWD was determined by ultraperformance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS E ) (7), and compounds were selected according to the Lipinski's rule of five (13)-MWT ≤ 500, H-bond donors ≤ 5, H-bond acceptors ≤ 10, and logP ≤ 5-to select the most likely active ingredients. Moreover, several active ingredients with relative high content or excellent bioactivity, which did not satisfy these criteria, were also manually supplemented as candidate compounds for further analysis (14).…”

Section: Screening Of Active Ingredients Of Thswdmentioning

confidence: 99%

Exploration of the Potential Mechanism of Tao Hong Si Wu Decoction for the Treatment of Breast Cancer Based on Network Pharmacology and In Vitro Experimental Verification

et al. 2021

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BackgroundTao Hong Si Wu Decoction (THSWD) is a well-known traditional Chinese medicine used clinically alone or combined with drugs to treat breast cancer. However, there has been no study to date on the underlying mechanisms of its therapeutic effects.ObjectivesTo explore the potential mechanism of THSWD for the treatment of breast cancer using network pharmacology and experimental research.MethodsThe active ingredients of THSWD were screened according to Lipinski’s rule of five based on the 107 ingredients of THSWD identified by UPLC-Q-TOF-MSE. The targets of THSWD and breast cancer from multiple databases were collected, and a Compound-Target-Pathway network based on protein-protein interaction (PPI) was constructed. Gene ontology (GO) analysis and KEGG pathway analysis were performed via the DAVID server. Molecular docking studies verified the selected key ingredients and key targets. The results of network pharmacology were verified by in vitro experiments. Including the effects of THSWD drug-containing rat serum (THSWD serum) on cell proliferation, and on the targets HRAS, MAPK1, AKT1, GRB2, and MAPK14 were assayed by RT-qPCR and Western blot assays.ResultsIn total, 27 active ingredients including 8 core components, were obtained from 107 ingredients and 218 THSWD target genes for the treatment of breast cancer were identified. THSWD is active in the treatment of breast cancer by targeting Ras, FoxO, PI3K-Akt and other signaling pathways. MCF-7 and MDA-MB-231 cell proliferation was inhibited by THSWD serum in a time and concentration dependent manner. THSWD could regulated the RNA and protein expression of core targets HRAS, MAPK1, AKT1, GRB2, and MAPK14 for treatment of breast cancer.ConclusionThe results of network pharmacology study showed that THSWD is active against breast cancer by intervening with multiple targets and pathways. Luteolin, kaempferol, senkyunolide E, and other 8 compounds may be the core active ingredients of THSWD in the treatment of breast cancer. THSWD treatment of breast cancer may be related to targeting Ras, FoxO, PI3K-Akt, and other signal pathways associated with the core targets HRAS, MAPK1, AKT1, GRB2, and MAPK14.

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Simultaneous qualitative and quantitative analysis of eight alkaloids in Corydalis Decumbentis Rhizoma (Xiatianwu) and Corydalis Rhizoma (Yanhusuo) by high‐performance liquid chromatography and high‐resolution mass spectrometry combined with chemometric methods

Zhong

et al. 2022

J of Separation Science

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In this study, we established a comprehensive high‐performance liquid chromatography coupled with diode array detection and high‐resolution mass spectrometry method to identify 10 and quantified eight constituents in Corydalis Decumbentis Rhizoma (“Xiatianwu” in Chinese) and Corydalis Rhizoma (“Yanhusuo” in Chinese). Chemometric methods were applied to distinguish the botanical origins of the Xiatianwu and Yanhusuo samples. Chromatographic separation was achieved using an Agilent Poroshell EC‐C18 column with mobile phases A (1000 ml of 0.2% acetic acid solution containing 2.8 ml of triethylamine) and B (acetonitrile) and stepwise gradient elution. The analytical method was fully validated in terms of linearity, sensitivity, intra‐ and interday precision and repeatability, the limit of detection, the limit of quantitation, and recovery. Twenty‐six Xiatianwu samples and 10 Yanhusuo samples were analyzed for quality evaluation. In addition, hierarchical clustering analysis and principal component analysis were used to discriminate among samples of different botanical origins. The results showed that the contents of eight alkaloids in Xiatianwu and Yanhusuo were significantly different. Moreover, it was found that chemometric methods could be applied to accurately distinguish these two often conflated Chinese medicinal materials. In conclusion, this study provides a relatively comprehensive method for botanical origin identification and Xiatianwu and Yanhusuo quality control.

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Rapid profiling of alkaloid analogues in Sinomenii Caulis by an integrated characterization strategy and quantitative analysis

Cited by 33 publications

References 31 publications

Discovery of chemical markers for improving the quality and safety control of Sinomenium acutum stem by the simultaneous determination of multiple alkaloids using UHPLC-QQQ-MS/MS

Discovery of chemical markers for improving the quality and safety control of Sinomenium acutum stem by the simultaneous determination of multiple alkaloids using UHPLC-QQQ-MS/MS

Exploration of the Potential Mechanism of Tao Hong Si Wu Decoction for the Treatment of Breast Cancer Based on Network Pharmacology and In Vitro Experimental Verification

Simultaneous qualitative and quantitative analysis of eight alkaloids in Corydalis Decumbentis Rhizoma (Xiatianwu) and Corydalis Rhizoma (Yanhusuo) by high‐performance liquid chromatography and high‐resolution mass spectrometry combined with chemometric methods

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