Rapid production of l‐DOPA by Vibrio natriegens, an emerging next‐generation whole‐cell catalysis chassis

Abstract

Summary 3, 4‐Dihydroxyphenyl‐ l ‐alanine ( l ‐DOPA) is a compound of high medical value and is considered effective as a treatment for Parkinson’s disease. Currently, bioproduction of l ‐DOPA is mainly carried out by whole‐cell catalysis mediated by recombinant Escherichia coli carrying heterogeneous tyrosine phenol lyase. Vibrio natriegens is increasingly attracting attention owing to its superiority, including extremely rapid growth and high soluble protein expression capacity. In this study, we attempt to develop an efficient whole‐cell catalyst for l ‐DOPA production using V. natriegens as the chassis. The maximum soluble protein expression by V. natriegens was accomplished in 4 h at 37°C, which was equivalent to that achieved by E. coli in 16 h at 16°C. Furthermore, the maximum productivity reached over 10.0 g l −1 h −1 in the early stage of biocatalysis, nearly two‐fold higher than previously reported. Approximately 54.0 g l −1 l ‐DOPA was obtained with a catechol conversion rate greater than 95%. In conclusion, V. natriegens displays advantages, including rapid protein expression and catalytic rate in the catalysis process for l ‐DOPA production. These findings strongly suggest that V. natriegens has remarkable potential as a whole‐cell catalysis chassis for the production of valuable chemicals.

Isolation and Characterization of a Novel Vibrio natriegens—Infecting Phage and Its Potential Therapeutic Application in Abalone Aquaculture

“…In addition, V. natriegens is a facultative anaerobe well adapted to both oxic and anoxic environments [ 54 , 55 ]. Thus, V. natriegens has been explored for various bioengineering and biotechnological applications [ 52 , 54 ], such as serving as a whole-cell catalysis chassis for the production of L-DOPA (3,4-dihydroxyphenyl-L-alanine, a promising drug for Parkinson’s disease), violacein, β-carotene, melanin, and other pharmaceuticals and valuable chemicals [ 56 , 57 , 58 , 59 ]. V. natriegens has also been tested as an engineered “live antimicrobial” for the control of bacterial pathogens [ 60 ].…”

High-Performance Production of N-Acetyl-d-Neuraminic Acid with Whole Cells of Fast-Growing Vibrio natriegens via a Thermal Strategy

“…Specifically, the plasmid pETDuet-Txage-EcnanA was introduced into V. natriegens Vmax, resulting in V. natriegens Vmax (pETDuet-Txage-EcnanA). Protein-induced expression was conducted at 37 °C for 4 h. 21 Surprisingly, the reactions performed beyond our expectation. As shown in Figure 5C, at concentrations of 1.6 M for pyruvate and 1.2 M for GlcNAc, the maximum Neu5Ac titer of 115.7 g/L was achieved at 6 h, with an average productivity of 19.3 g/(L h), twice as high as that of E. coli BL21(DE3) (pETDuet-Txage-EcnanA) in the same reaction time (Figure 5C).…”

Taking Synthetic Biology to the Seas: From Blue Chassis Organisms to Marine Aquaforming

“…Consequently, the first studies that describe biotechnological production of value‐added molecules using V. natriegens as a host have been conducted in the past years (Figure 1a). Recently, the production of 3,4‐dihydroxyphenylalanine ( l ‐DOPA) from catechol, pyruvate, and acetate, [40] the conversion of glycerol into propane‐1,3‐diol, [14] and the anaerobic production of succinate from glucose [41] were reported. More complex molecules, such as β‐carotene and violacein, were also produced using engineered strains of this marine bacterium [42] .…”