Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C

Sharma, Sandeep; Hanif, Sarmad; Kumar, Nitin; Joshi, Neeraj; Rana, Rakesh; Dalal, Juned; Singh, Deepti; Chhikara, Manoj Kumar

doi:10.1016/j.biologicals.2015.06.003

Cited by 6 publications

(4 citation statements)

References 21 publications

(16 reference statements)

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“…In general, the CPS purification processes developed for S. pneumoniae, Haemophilus influenza type b, and Neisseria meningitides in recent decades have achieved increased efficiency by reducing the number of ethanol precipitations employed and eliminating the use of phenol (Suárez et al, 2001 ; Pato et al, 2006 ; Gonçalves et al, 2007 ; Albani et al, 2012 , 2015 ; Sharma et al, 2015 ). Particularly for Neisseria meningitidis , Tanizaki et al ( 1996 ) proposed a modification of the classical purification process for meningococcal group C polysaccharides by substituting the phenol extraction step by digestions with proteases and extensive diafiltration.…”

Section: Discussionmentioning

confidence: 99%

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Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods

Morais

Dee

Suárez

2018

Front. Bioeng. Biotechnol.

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Pneumonia caused by Streptococcus pneumoniae is a major bacterial disease responsible for many deaths worldwide each year and is particularly dangerous in children under 5 years old and adults over 50. The capsular polysaccharide (CPS) constitutes the outermost layer of the bacterial cell and is the main virulence factor. Regardless of whether pharmaceutical agents are composed of CPS alone or protein-conjugated CPS, CPS purification is essential for the development of vaccines against S. pneumoniae. These vaccines are effective and safe but remain quite expensive. This review describes the methods currently available for CPS purification. Advances in CPS purification methods are aimed at improvements in quality and yield and, above all, process simplification.

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Section: Discussionmentioning

confidence: 99%

“…Recently, Sharma and collaborators attempted to simplify the purification process of bacterial polysaccharides for serogroups A and C of N. meningitidis by utilizing O-acetylation and hydrophobic interaction chromatography (HIC), with good results (Sharma et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods

Morais

Dee

Suárez

2018

Front. Bioeng. Biotechnol.

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“…For pharmaceuticals, potency can directly link quantity of the active substance and the product's desired therapeutic effect. The picture is less clear for cell-based products, where the definition of potency needs adaptation to fit the specific properties of cell therapies to also include measurements of viability, self-renewal, death, and differentiation [ 15 ]. Definitions of potency can be found in the 1999 European Medicines Agency (EMA) ICH Q6B guidelines, as well as the 2011 Guidance for Industry from the US Department of Health and Human Services Food and Drug Administration (FDA) “Potency Tests for Cellular and Gene Therapy Products” (CGT) [ 16 ].…”

Section: The Challenge Of Potency In Cell-based Therapeuticsmentioning

confidence: 99%

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Amărandi

Becheru

Vlăsceanu

et al. 2018

BioMed Research International

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Regenerative medicine is challenged by the need to conform to rigorous guidelines for establishing safe and effective development and translation of stem cell-based therapies. Counteracting widespread concerns regarding unproven cell therapies, stringent cell-based assays seek not only to avoid harm but also to enhance quality and efficacy. Potency indicates that the cells are functionally fit for purpose before they are administered to the patient. It is a paramount quantitative critical quality attribute serving as a decisive release criterion. Given a broad range of stem cell types and therapeutic contexts the potency assay often comprises one of the most demanding hurdles for release of a cell therapy medicinal product. With need for improved biomarker assessment and expedited measurement, recent advances in graphene-based biosensors suggest that they are poised to be valuable platforms for accelerating potency assay development. Among several potential advantages, they offer versatility for sensitive measurement of a broad range of potential biomarker types, cell biocompatibility for direct measurement, and small sample sufficiency, plus ease of use and point-of-care applicability.

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“…Additionally, a great amount of N. meningitidis (Serogroup C) polysaccharide is needed. The cost for cultivation and the production of polysaccharide is generally high and involves a series of production and purification steps [ 18 ]. However, little information is available in the literature about the production of this PS on pilot or industrial scale [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

Baruque-Ramos

Arauz

Paz

et al. 2016

CBE

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Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (Pf), as well as the overall yield factor (PS/biomass) (YP/X), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35oC, 0.4 atm, 120 rpm, airflow rate of 5 L/min and KLa = 4.2 h-1. The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O2 controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10th hour; D) dissolved O2 control at 10% saturation value; E) pH control at 7.4; F) dissolved O2 limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of Pf = 0.15 g/L and YP/X = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.

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Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C

Cited by 6 publications

References 21 publications

Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods

Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

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