Rapid presumptive diagnosis of gonococcal urethritis in men by the limulus lysate test.

Spagna, Vincent A.; Prior, Richard B.; Perkins, Robert L.

doi:10.1136/sti.55.3.179

Cited by 20 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent attempts to provide a non-cultural diagnosis for gonorrhoea have included the detection of antibodies against gonococci (Buchanan et al, 1973;O'Reilly, Welch and Kellogg, 1973;Oates et al, 1977) as well as the detection of gonococcal components such as deoxyribonucleic acid (Janik, Juni and Heym, 1976;Sarafian and Young, 1980), endotoxin (Spagna, Prior and Perkins, 1979;Young, Sarafian and McMillan, 198 l), and surface antigens (Thornley et al, 1979). Although these studies have met with only limited success, a reliable non-cultural method remains a worthwhile goal in gonococcal research.…”

Section: Introductionmentioning

confidence: 99%

Detection of Gonococcal Antigens by an Indirect Sandwich Enzyme-linked Immunosorbent Assay

Sarafian

Young

1982

Journal of Medical Microbiology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Detection of Gonococcal Antigens by an Indirect Sandwich Enzyme-linked Immunosorbent Assay

Sarafian

Young

1982

Journal of Medical Microbiology

View full text Add to dashboard Cite

“…Therefore, the LAL test has been used in clinical medicine for the rapid detection of gram-negative bacteria in a number of body fluids such as: blood, 6 " 9 cerebrospinal fluid, 10 " 13 synovial fluid, 13 " 15 urine, 1617 and urethral 18 and cervical exudates. 19 The main appeal of this test is that it can frequently detect infection even when organisms are not seen on the gram stain examination. This permits the early initiation of antibiotic therapy in some life-threatening infections (e.g., meningitis).…”

mentioning

confidence: 99%

Do Blood and Meconium Affect the Detection of Endotoxin in Amniotic Fluid with the Limulus Amebocyte Gel Clot Assay?

Romero¹,

Kadar²,

Lafreniere³

et al. 1987

Amer J Perinatol

View full text Add to dashboard Cite

The limulus amebocyte lysate (LAL), assay is the most sensitive technique for the detection of endotoxin in biological fluids. Because endotoxin is a component of gram-negative bacteria, the assay has been employed in the detection of gram-negative bacterial contamination of biological fluids. The LAL assay is rapid, inexpensive, easy to perform, and requires little laboratory expertise. When used in conjunction with the gram stain examination of amniotic fluid, it improves the detection of intra-amniotic infection before the availability of culture results. However, the usefulness of the LAL assay in the detection of endotoxin in other body fluids is limited by the presence of an inhibitor to the gelation of the assay. The studies reported in this communication were undertaken to establish if amniotic fluid contains such an inhibitor. Sterile amniotic fluid (AF) samples obtained from 93 patients by transabdominal amniocentesis before labor were used to determine the ED 50 dose of endotoxin necessary for a positive LAL result. The ED 50 dose of endotoxin required for gelation was significantly higher when AF--rather than pyrogen-free saline--was used as the diluent, implying that inhibitors are in fact present (ED 50 = 58.3 pgm/ml). The presence of blood or meconium in the AF did not enhance inhibition significantly: ED 50 doses were 58.3 pgm/ml and 56.2 pgm/ml, respectively. This is not significantly different from the ED 50 of clear amniotic fluid.

show abstract

“…The test for this reaction is known as the Limulus amoebocyte lysate (LAL) test and currently constitutes the most sensitive method available for detection of bacterial endotoxin. The LAL test has been shown to have potential diagnostic value for the rapid screening of a variety of body fluids for gram-negative infectious disease (13)(14)(15)(16)(17)(26)(27)(28) and has been adopted for endotoxin detection in pharmaceutical drugs and medical devices (12,21,31). The unique properties of LAL include its sensitivity to and reported specificity for endotoxin; however, specificity remains a controversial issue as a result of reports of LAL activation (gelation) by substances other than endotoxin (1,5,6,11,29,34).…”

mentioning

confidence: 99%

Establishment of beta-hydroxy fatty acids as chemical marker molecules for bacterial endotoxin by gas chromatography-mass spectrometry

Maitra¹,

Nachum²,

Pearson³

1986

Appl Environ Microbiol

View full text Add to dashboard Cite

Selected ion-monitoring gas chromatography-mass spectrometry was used for detection of beta-hydroxy fatty acids as an independent assay for the presence or absence of endotoxin in materials claimed to induce nonspecific activation of Limulus amoebocyte lysate. To this end, suspensions of gram-negative and-positive bacteria, one fungal species, cerebrospinal fluid, and hollow-fiber hemodialyzer rinses were assayed for endotoxin by gas chromatography-mass spectrometry and the Limulus amoebocyte lysate assay. Good qualitative agreement was shown for both methods when suspensions of test organisms were assayed. Two false-negative results were obtained by gas chromatography-mass spectrometry assays of cerebrospinal fluid and were shown to be a result of insufficient endotoxin in the cerebrospinal fluid specimens for detection by gas chromatography-mass spectrometry. Hemodialyzer rinses were Limulus assay positive; however, no betahydroxy fatty acids were detected by gas chromatography-mass spectrometry. These data were compared with data obtained from USP rabbit pyrogen tests of the rinse materials (nonpyrogenic) and chemical characterization of the Limulus assay-reactive rinses, which showed the rinses to be cellulosic in nature. It is suggested that beta-hydroxy fatty acids, as assayed by selected ion-monitoring gas chromatography-mass spectrometry, be used as chemical marker molecules for the presence or absence of endotoxin in materials reported to cause nonspecific activation of Limulus amoebocyte lysate.

show abstract

Rapid presumptive diagnosis of gonococcal urethritis in men by the limulus lysate test.

Cited by 20 publications

References 23 publications

Detection of Gonococcal Antigens by an Indirect Sandwich Enzyme-linked Immunosorbent Assay

Detection of Gonococcal Antigens by an Indirect Sandwich Enzyme-linked Immunosorbent Assay

Do Blood and Meconium Affect the Detection of Endotoxin in Amniotic Fluid with the Limulus Amebocyte Gel Clot Assay?

Establishment of beta-hydroxy fatty acids as chemical marker molecules for bacterial endotoxin by gas chromatography-mass spectrometry

Contact Info

Product

Resources

About