Rapid point–of–care diagnosis of chikungunya virus infection

Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus

“…CHIKV and DENV diagnosis may be approached via several methods such as conventional RT-PCR, commercial rapid test, ELISA, HI test, virus culture and antibody screening (IgM, IgG). Screening for antibody of virus is commonly used but IgM of CHIKV may cross-react with other alphaviruses such as O'nyong nyong virus [26][27][28] and the antibody of DENV may cross react with other members of the Flaviviridae family such as JEV [29], resulting in false positives. Virus culture has shown reliable results but is laborious and time consuming to perform.…”

“…A total of 290 suspected cases were recruited in this study as well, comprised of acute serum samples collected within 7 days of the onset of illness, consistent with clinical diagnosis of dengue according to WHO criteria [23] and of CHIKV samples from suspected infections in the endemic area. Moreover, 28 hepatitis C positive, 2 hepatitis A positive, 6 hepatitis E positive, 2 Japanese encephalitis virus (JEV) samples were included in this study to evaluate sensitivity and specificity of this method.…”

Detection of chikungunya virus‐specific IgM on laser‐cut paper‐based device using pseudo‐particles as capture antigen

“…Clinical performances were calculated from a small cohort of patients (N = 78) and need to be confirmed on a larger cohort. Equivalent sensitivity was observed by Arya et al for the same SD Bioline kit. By increasing the reading time to 20 minutes, as suggested by the manufacturer, the sensitivity of the SD Bioline test dropped to 68.2% .…”

“…However, for the SD Bioline Kit, the sensitivity reached 68.2% by increasing the time‐to‐result to 20 minutes instead of 10 minutes. Arya SC et al evaluated the performances of the CTK Biotech kit as well, 69 and calculated sensitivity and a specificity of 71% and 100%, respectively. For the authors, these commercial kits are essential in healthcare centers for developing countries, to be used in the routine early diagnosis and to initiate control measures for CHIK.…”

Metanálisis sobre la utilidad de ELISA, PCR e inmunocromatografía en el diagnóstico de chikungunya

“…Los estudios basados en detección de ácidos nucleicos del virus mostraron menor variabilidad en los valores de sensibilidad, especificidad, CPP, CPN y ORD, al tiempo que puede clasificarse como excelente ayuda diagnóstica según los parámetros de CPP mayor a 10, CPN menor a 0,1 y ORD mayor a 100; lo que implica mayor posibilidad de generalización de los resultados, siempre y cuando se tenga presente que en este metanálisis los genes del virus más usados fueron los de envoltura (E) (4,11,14,16,17,20,21,25)y las proteínas no estructurales del virus (nsP) (12,18-19). Por su parte, los valores de sensibilidad y especificidad de la inmunocromatografia son similares a lo encontrado en estudios previos que reportan baja utilidad para el diagnóstico de CHIKV, con sensibilidades del 12,2% al 30%; incluso Prat y cols.…”