2014
DOI: 10.1186/1471-2180-14-84
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Rapid optical determination of β-lactamase and antibiotic activity

Abstract: BackgroundThe absence of rapid tests evaluating antibiotic susceptibility results in the empirical prescription of antibiotics. This can lead to treatment failures due to escalating antibiotic resistance, and also furthers the emergence of drug-resistant bacteria. This study reports a rapid optical method to detect β-lactamase and thereby assess activity of β-lactam antibiotics, which could provide an approach for targeted prescription of antibiotics. The methodology is centred on a fluorescence quenching base… Show more

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Cited by 34 publications
(34 citation statements)
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“…At present, the research shows that β-lactam antibiotics have a lethal effect on bacteria mainly through two mechanisms: first, by binding to penicillin-binding protein (PBPs, i.e., cell wall mucin synthase), which represses cell wall mucin synthesis, disrupts the cell wall, and leads to bacterial expansion and lysis; second, by triggering the autolytic enzyme activity of the bacteria, which resulted in autolysis and death (Matono et al, 2018). Excessive secretion of β-lactamase by MRSA mainly reduces the effect of antibiotics through two mechanisms, which lead to MRSA resistant (Khan et al, 2014). The first is the hydrolysis mechanism, that is, β-lactamase hydrolzes and inactivates β-lactam antibiotics; the second is the mechanism of pinching, that is, a large amount of β-lactamase binds quickly and firmly to extracellular antibiotics, preventing the antibiotics from reaching the intracellular space and therefore the antibiotics are not able to reach the target site, ultimately leading to MRSA resistance to antibiotics ( Figure 1D) (Harada et al, 2014;Hashizume et al, 2017).…”
Section: Excessive Production Of β-Lactamasementioning
confidence: 99%
“…At present, the research shows that β-lactam antibiotics have a lethal effect on bacteria mainly through two mechanisms: first, by binding to penicillin-binding protein (PBPs, i.e., cell wall mucin synthase), which represses cell wall mucin synthesis, disrupts the cell wall, and leads to bacterial expansion and lysis; second, by triggering the autolytic enzyme activity of the bacteria, which resulted in autolysis and death (Matono et al, 2018). Excessive secretion of β-lactamase by MRSA mainly reduces the effect of antibiotics through two mechanisms, which lead to MRSA resistant (Khan et al, 2014). The first is the hydrolysis mechanism, that is, β-lactamase hydrolzes and inactivates β-lactam antibiotics; the second is the mechanism of pinching, that is, a large amount of β-lactamase binds quickly and firmly to extracellular antibiotics, preventing the antibiotics from reaching the intracellular space and therefore the antibiotics are not able to reach the target site, ultimately leading to MRSA resistance to antibiotics ( Figure 1D) (Harada et al, 2014;Hashizume et al, 2017).…”
Section: Excessive Production Of β-Lactamasementioning
confidence: 99%
“…However, the rate of cleavage was not as pronounced with cefepime, indicating this antibiotic as being more robust against cleavage for this particular \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\beta $ \end{document}-lactamase (as compared to the other cephalosporins). Gold standard assays of antibiotic susceptibility have confirmed cefepime to be the most effective antibiotic out of the 3 for this pathogen strain [30], supporting the use of the microfluidic as a rapid platform for the \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\beta $ \end{document}-LEAF antibiotic susceptibility assay.
FIGURE 8.Microfluidic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$\beta $ \end{document}-LEAF antibiotic susceptibility assay.
…”
Section: Resultsmentioning
confidence: 82%
“…This phenomenon leads to the development of various defensive mechanisms able to inactivate the applied chemical substance [7]. The most common resistant strain, which evolved from the Staphylococcus aureus , is methicillin-resistant S. aureus (MRSA) [8,9]. The incidence of MRSA in hospitalized patients results particularly from an intensive and long-term therapy [10], where S. aureus is exposed to relatively high concentrations of antibiotics for a longtime, thus certain mutations can develop.…”
Section: Introductionmentioning
confidence: 99%