Rapid on-line microdialysis hyphenated technique for the dynamic monitoring of extracellular pyruvate, lactic acid and ascorbic acid during cerebral ischemia

Cheng, Fu‐Chou; Yang, Dar-Yu; Wu, Tsu-Fang; Chen, Sy-Huah

doi:10.1016/s0378-4347(98)00532-5

Cited by 27 publications

(6 citation statements)

References 19 publications

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“…Although microdialysis has been very useful for analyzing the tissue and biological fluid concentrations of a number of pharmacological agents, neurotransmitters and metabolites, the technique has been limited to relatively small molecular weight compounds 1,2,4,5,8–13. The commercial availability of larger molecular weight cutoff microdialysis probes (up to 100 kDa) has made it possible to consider a wider range of compounds for microdialysis.…”

Section: Introductionmentioning

confidence: 99%

Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules

Trickler

Miller

2003

Journal of Pharmaceutical Sciences

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Section: Introductionmentioning

confidence: 99%

Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules

Trickler

Miller

2003

Journal of Pharmaceutical Sciences

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“…Microdialysis has the advantage of being minimally invasive when it is used to collect in vivo molecules in human dermis, the opposite of Shindo et al's procedure. 7 Cheng et al 21 have shown that AA was maintained at a relatively stable level after probe implantation in the brain. Similar results were obtained in this work: probe insertion did not cause a significant variation in AA levels in extracellular fluid from human skin.…”

Section: Assessment Of Aa Concentration In Human Dermismentioning

confidence: 99%

Ascorbic Acid Assessment in Human Dermis by a Microdialysis Technique Associated with Gas Chromatography-Mass Spectrometry

Lévêque

Mary

Humbert

et al. 2000

Eur J Mass Spectrom (Chichester)

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Ascorbic acid (AA) plays a significant role in preventing photobiological damage in human skin which could lead to cutaneous disorders such as premature aging or skin cancer. The aim of this work was to assess AA concentrations in human dermis by a microdialysis technique associated with gas chromatography-mass spectrometry (GC-MS). Due to the non-volatility of AA, it was necessary to derivatize this acid. Three methods, one methylation and two silylations, were validated and compared to determine the most sensitive. To validate the methods, calibration curves were plotted from AA concentrations ranging from 34 to 500 nmol mL-1. The calibration curves were linear with a good correlation coefficient (r ≥ 0.99). Repeatability and reproducibility were significant with a coefficient of variation of less than 10%. The accuracy of the techniques was meaningful as the bias was low (ranging from-5.6 to 5.0%). According to our results, silylation was the most sensitive method to assess AA. Thus, this method was performed to determine AA concentrations in microdialysates. In order to study AA in human dermis, a microdialysis method was used to sample AA and the GC-MS technique used to assess this acid in the microdialysates. Microdialysis can only partially collect AA from human dermis. An ex vivo recovery of AA was 30 ± 2% (n = 7). The average concentration of AA in microdialysates was 250 ± 66 nmol mL-1 (Mean ± SD, n = 5). In view of the AA recovery, AA concentrations in human skin ranged from 759 to 891 nmol mL-1 .

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“…This has resulted in abundant literature from studies in carefully controlled animal models that quantitates neurochemical changes in focal cerebral ishemia. Over the last few years, extension of laboratory-based studies have been reported [2][3][4][5] in patients with large hemispheric infarctions, traumatic brain injury, 6 global cerebral ischemia 7 and subarachnoid hemorrhage 8,9 . Neuroprotective strategies, 10 including hypothermia, [11][12][13] and their effects on neurotransmitter release have been well-described using this technique.…”

Section: In Vivo Regional Neurochemistry In Strokementioning

confidence: 99%

“…Second, the time resolution (sampling time) of cerebral microdialysis in our study was 120 minutes. The method, however, allows higher time resolution 8,9 or even continuous measurement. 10 In our study, we used microdialysis to get information over an 80-hour time period, in which malignant brain edema develops.…”

Section: Responsementioning

confidence: 99%

Cerebral Microdialysis in Stroke Patients: Potentials and Limitations of a Method with Longitudinal Information: Response

Bosche¹

2004

Stroke

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Rapid on-line microdialysis hyphenated technique for the dynamic monitoring of extracellular pyruvate, lactic acid and ascorbic acid during cerebral ischemia

Cited by 27 publications

References 19 publications

Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules

Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules

Ascorbic Acid Assessment in Human Dermis by a Microdialysis Technique Associated with Gas Chromatography-Mass Spectrometry

Cerebral Microdialysis in Stroke Patients: Potentials and Limitations of a Method with Longitudinal Information: Response

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