Rapid neutralization assay for human cytomegalovirus antibody

Abstract

Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunQfluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the * Corresponding author.

Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein

“…In the past, the need comparable for time-consuming plaque-reduction neutralization assays alizing titer has inhibited the study of neutralizing antibody in various ggests that patient populations. Newer, rapid assays such as those described here and elsewhere (11,28), should facilitate these studies, but each new method must be calibrated against 1 established neutralization assays, tested for applicability to a 1 4…”

Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples

“…To a large extent, serological assays represent the most common method for the laboratory diagnosis of CMV infections (11,17,18). In fact, serum samples can easily be obtained, and several immunological assays are commercially available.…”