Rapid molecular diagnosis of live Mycobacterium tuberculosis on an integrated microfluidic system

Specific identification of Mycobacterium bovis by Loop-Mediated Isothermal Amplification (LAMP) targeting the Region of Difference 12 (RD12) of the M. tuberculosis complex

“…Specifically, PCR-based techniques have several advantages in terms of speed, which means rapid diagnosis and efficient control. Despite the fact that PCR-based techniques require specialized equipment and qualified staff to obtain reliable results, those techniques overcome the specificity limitations of traditional methods used for mycobacteria typing such as cultures or biochemical tests [29] . Nevertheless, molecular techniques based on PCR are expensive, and sometimes, in the case of zoonotic infections, are difficult to perform there where livestock production centers are located, especially in developing countries.…”

Microfluidic systems for infectious disease diagnostics

“…Bacteria capture, thermolysis and DNA release, PMA treatment and rpoB gene PCR amplification was performed on-chip within 90 min and a reported LOD of 100 CFU. 223 Kukhtin et al developed a disposable lab-on-a-film that detects MDR-TB from sputum extracts. 224 The device comprises a gel-based microarray printed onto a flexible polyester film.…”

Microfluidics for Rapid Detection of Live Pathogens

“…Wang et al developed a PDMS chip that was able to detect a total of 100 CFU in 90 min, incorporating on-chip capture of M. tuberculosis cells using a heparin-binding hemagglutinin antibody and live cell detection via PMA treatment. [59] The chip contained 12 PCR reaction chambers to further carry out onchip PCR and the fluorescence signal was detected with a laserinduced fluorescent module. In order to automate the process, micromixers as well as micropumps were used as external components.…”