Rapid molecular diagnosis of infective aortic valve endocarditis caused by Coxiella burnetii

Kumpf, Oliver; Dohmen, Pascal M.; Ertmer, Martin; Knebel, Fabian; Wießner, Alexandra; Kikhney, Judith; Moter, Annette; Treskatsch, Sascha

doi:10.1007/s15010-016-0916-9

Cited by 15 publications

(9 citation statements)

References 18 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we proved that both FISH and PNA FISH gave better results than immunohistochemistry for the diagnosis of C. burnetii endocarditis and vascular infections. Recently, FISH and molecular assays also enabled the diagnosis of Q fever endocarditis in a patient with severe destruction of the aortic valve with perivalvular abscess formation and cardiac failure (10). Similarly, Aistleitner et al diagnosed a case of Q fever endocarditis by using FISH as well (12).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, immunohistochemistry often fails to confirm the diagnosis, particularly as a result of early antibiotic treatment. In recent years, fluorescence in situ hybridization (FISH) has been proposed for the diagnosis of Q fever endocarditis using the pan-bacterial probe in both humans (10) and animals (11). Recently, the detection of C. burnetii in heart valve tissues by FISH has also been reported (12).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections

et al. 2018

View full text Add to dashboard Cite

Endocarditis and vascular infections are common manifestations of persistent localized infection due to , and recently, fluorescence hybridization (FISH) was proposed as an alternative tool for their diagnosis. In this study, we evaluated the efficiency of FISH in a series of valve and vascular samples infected by We tested 23-positive valves and thrombus samples obtained from patients with Q fever endocarditis. Seven aneurysms and thrombus specimens were retrieved from patients with Q fever vascular infections. Samples were analyzed by culture, immunochemistry, and FISH with oligonucleotide and PNA probes targeting -specific 16S rRNA sequences. The immunohistochemical analysis was positive for five (17%) samples with significantly more copies of DNA than the negative ones ( = 0.02). FISH was positive for 13 (43%) samples and presented 43% and 40% sensitivity compared to that for quantitative PCR (qPCR) and culture, respectively. PNA FISH detected in 18 (60%) samples and presented 60% and 55% sensitivity compared to that for qPCR and culture, respectively. Immunohistochemistry had 38% and 28% sensitivity compared to that for FISH and PNA FISH, respectively. Samples found positive by both immunohistochemistry and PNA FISH contained significantly more copies of DNA than the negative ones ( = 0.03). Finally, PNA FISH was more sensitive than FISH (60% versus 43%, respectively) for the detection of We provide evidence that PNA FISH and FISH are important assays for the diagnosis of endocarditis and vascular infections.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections

et al. 2018

View full text Add to dashboard Cite

show abstract

“…A comparison to the SILVA 16S rRNA database ( Quast et al, 2013 ) revealed that at least one of the reported probes has a perfect match to over 8000 non-target species in the respective region of the 16S rRNA of different bacterial families ( Melenotte et al, 2016 ), resulting in false-positive hybridization signals for all of those species. In the absence of properly tested probes, a previous study used the general bacterial probe EUB ( Amann et al, 1990 ) in conjunction with PCR to diagnose C. burnetii in a patient with IE ( Kumpf et al, 2016 ). The high autofluorescence level of the heart valve tissue was a concern in this research and other studies analyzing tissue parts of endocarditis patients ( Gescher et al, 2008 ).…”

Section: Molecular Basis Of Antimicrobial Resistance In Infective Endocarditismentioning

confidence: 99%

Infective endocarditis: Role of molecular techniques in early diagnosis

Albloshi

Alqumber

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Infective endocarditis (IE), a heart valve infection primarily caused by bacteria such as streptococci or staphylococci , causes significant morbidity and mortality. Despite the long-term use of broad-spectrum antimicrobials, the infection is often difficult to manage. The latest diagnostic modalities for IE are discussed in this study. Blood culture use in pathogen identification can lead to loss of precious time as well as generation of false negative reports. The first steps in diagnosis are blood cultures and echocardiography, but molecular techniques can be extremely useful and may be used for an accurate and early diagnosis.

show abstract

“…The first report on the rapid diagnosis of Q fever endocarditis by PCR of aortic valve tissue was recently published (Kumpf et al, 2016), and a retrospective review of resected cardiac valves/ prostheses from 6401 patients undergoing valve surgery led to an unexpected diagnosis of latent Q fever endocarditis in 14 cases (0.2%) (Grisoli et al, 2014). The case reported here highlights the possibility of identifying and typing C. burnetii in clinical microbiology laboratories without access to biosafety level 3 containment.…”

Section: Discussionmentioning

confidence: 99%

Multispacer sequence typing of Coxiella burnetii DNA from removed prosthetic heart valve material discloses first human case of infective endocarditis caused by MST_18

Nielsen

Nørskov‐Lauritsen

2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Introduction: In Denmark, Q fever has previously been considered a rare and imported disease; however, recent testing of antibodies in cattle as well as humans has indicated that the infection is widespread. A 76-year-old Danish man was diagnosed with infective endocarditis and underwent open surgical aortic valve replacement with insertion of a biological valve. Due to paravalvular leakage, destruction of the aortic annulus, and an aortic root abscess, the patient underwent re-operation 3 weeks later, with replacement of the biological valve and insertion of an aortic prosthetic tube. Despite treatment with various broad-spectrum antibiotic regimes, the patient died 3.5 months after initial hospital admission. Methods: The causative agent was probed by PCR amplification of bacterial DNA on the removed prosthetic aortic valve using broad range primers targeting the variable regions V1-V3 of the 16S RNA gene. After identification of Coxiella burnetii, multispacer sequence typing (MST) was performed by PCR amplification of 10 intergenic sequences. Results: BLAST analysis of DNA from prosthetic valve material identified a 16S rRNA gene fragment almost identical to the type strain of C. burnetii (462/463 nt). Molecular typing allocated the strain to MST_18. Conclusions: Molecular methods are increasingly used to characterize isolates and to determine relationships between isolates that cause disease in different contexts and geographical areas. The present case demonstrates that identification and typing of C. burnetii is achievable without access to biosafety level 3 containment and highlights the first molecular characterization of an uncultured strain of C. burnetii causing infective endocarditis.

show abstract

Rapid molecular diagnosis of infective aortic valve endocarditis caused by Coxiella burnetii

Cited by 15 publications

References 18 publications

Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections

Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections

Infective endocarditis: Role of molecular techniques in early diagnosis

Multispacer sequence typing of Coxiella burnetii DNA from removed prosthetic heart valve material discloses first human case of infective endocarditis caused by MST_18

Contact Info

Product

Resources

About