Rapid microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live cell staining, cell-specific targeting and in vivo imaging

He, Hua; Wang, Xiaojuan; Feng, Zhonglin; Cheng, Tiantian; Sun, Xing; Sun, Yawei; Xia, Yongqing; Wang, Shengjie; Wang, Junying; Zhang, Xiao Dong

doi:10.1039/c5tb00570a

Cited by 49 publications

(31 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthesis of highly uorescent carbon dots. Nitrogen doped carbon dots (NC-dots) was prepared based on the microwave irradiation method that developed previously by He et al 59 Briey, mixture of citric acid (CA, 0.02 M) and ethylenediamine (EDA, 0.82 M) was dissolved ultrasonically into 100 ml of distilled water. A clear and light yellow solution was obtained.…”

Section: Methodsmentioning

confidence: 99%

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

et al. 2017

View full text Add to dashboard Cite

In this study, hybrid carbon dots-plasmonic nanostructures including carbon dots/polyethyleneimine/gold (C-dots/PEI/Au), and carbon dots/polyethyleneimine/silver (C-dots/PEI/Ag) have been prepared using a microwave irradiation method. The prepared hybrid nanostructures have been characterized via optical spectroscopy, high resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD).A remarkable enhancement in the optical parameters, such as absorptivity and quantum yield (QY), has been observed for the hybrid nanostructure compared to pure carbon dots. This plasmonic enhancement was more pronounced in the presence of silver (C-dots/PEI/Ag nanohybrid) than that of gold (C-dots/PEI/Au nanohybrid). This is referred to the low intrinsic loss and the degree of the overlap between the absorption spectra of silver nanoparticles and carbon dots. Furthermore, the biocompatibility assay and cellular response on epithelial kidney (Vero) normal cell has been investigated.The results showed that the optimal dose of treatment is about $200 mg ml À1 using both C-dots/PEI/Au or C-dots/PEI/Ag, nano-hybrids could be used safely in diagnostic bioimaging applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

et al. 2017

View full text Add to dashboard Cite

show abstract

“…CDs can be easily conjugated with targeting molecules for specific localization. For example, cyclic Arginyl-Glycyl-Aspartic acid (cRGD) peptides modified CDs showed effective accumulation on membrane surface and cytoplasm of tumor cells [106,107] . It is well known that cRGD specifically targets the integrin receptors which overexpress on the tumor cells while RGD recognize cell surface receptor integrin αvβ 3 of all cells [108].…”

Section: Fluorescent Cds Conjugated With Targeting Unitmentioning

confidence: 99%

Recent advance of carbon dots in bio-related applications

Wang

Bao

et al. 2020

J. Phys. Mater.

View full text Add to dashboard Cite

Carbon dots (CDs) is a kind of carbon nanoparticles with a plentiful of surface functional groups and tunable emission with different excitation wavelength. Broadly speaking, CDs include carbon nanodots, carbon quantum dots, graphene quantum dots, carbonized polymer dots. Due to the unique nature, they are explored for various applications in the bio-related fields such as bioimaging, sensor for ion and (bio)molecules, catalyst, LED and other fields. They are viewed as great alternative tracers to the current fluorescent biomarkers in personalized nanomedicine and surgery operation monitoring. In this review, we summarized the recent progress in the development of CDs, including improvement in fluorescence properties, two-photon fluorescence, and integration with other modalities as theragnostic agents. Specifically, we discussed the preparation of dual-modal imaging agents to improve the accuracy of diagnosis, the combination of imaging and targeting functionality for the effective accumulation of biomarkers, and the integration of imaging and therapeutic agents to effectively monitor the localization and concentration of therapeutic agents. Finally, the theragnostic agents composed of three functionalities (e.g. targeting, imaging, and therapy) were summarized to provide readers with future perspectives in this field.

show abstract

“…15–20 In most of the in vivo studies, however, C-dots were found to accumulate in various parts of the body without specificity. 14, 21–23 The lack of specificity significantly limits their use for targeted applications, such as early detection and treatment of bone de-mineralization diseases. Only recently, interactions of C-dots with ex vivo samples, extracted and cracked bones, have been reported using C-dots functionalized with glutamic acid.…”

Section: Introductionmentioning

confidence: 99%

Carbon dots: promising biomaterials for bone-specific imaging and drug delivery

et al. 2017

View full text Add to dashboard Cite

Bone-related diseases and dysfunctions are heavy burdens on our increasingly aged society. One important strategy to relieve this problem is through early detection and treatment of bone-related diseases. Towards this goal, there has been constant interest in developing novel bone-specific materials for imaging and drug delivery. Currently, however, materials that have high affinity and specificity towards bone are very limited. Carbon dots (C-dots) synthesized from carbon nanopowder bind to calcified bones in vivo with high affinity and specificity. In this study we show that bone binding is highly unique to a specific type of C-dots, and that this binding is non-toxic. Significantly, C-dots derived from other raw materials did not show any bone binding properties. These differences are attributed to differences in surface chemistry of C-dots preparations, highlighting the heterogeneous nature of C-dots. Importantly, bone-binding by carbon nanopowder derived C-dots are not significantly altered by chemical functionalization of their surface. These unique properties indicate the potential applications of carbon nanopowder-derived C-dots as highly bone-specific bioimaging agents and drug carriers.

show abstract

Rapid microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live cell staining, cell-specific targeting and in vivo imaging

Abstract: Ultra-bright fluorescent carbon dots were rapidly synthesized for multifunctional bio-imaging including live cell staining, cell-specific targeting and in vivo imaging.

Cited by 49 publications

References 28 publications

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

Recent advance of carbon dots in bio-related applications

Carbon dots: promising biomaterials for bone-specific imaging and drug delivery

Contact Info

Product

Resources

About