Rapid microwave assisted extraction of meat lipids

Medina, Aline Lisbôa; Silva, Marcelo Ancelmo Oseas da; Barbosa, Herbert de Sousa; Arruda, Marco Aurélio Zezzi; Marsaioli, Antonio; Bragagnolo, Neura

doi:10.1016/j.foodres.2015.10.028

Cited by 24 publications

(13 citation statements)

References 43 publications

(46 reference statements)

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“…They reported that the efficiencies of MAE, Bligh and Dyer, Folch, modified Folch and Hara & Radin methods were the highest whereas Roese‐Gottlieb, Soxhlet, and modified Bligh & Dyer methods were very poor in terms of efficiency. Medina, Silva, Barbosa, Arruda, Marsaioli, and Bragagnolo investigated MAE extraction of meat and reported no statistical differences in fat content of meat compared to the conventional extraction method (Folch method) and also did not change the fatty acid composition. Interactions between lipids and proteins, present naturally in tissues, occur in products during processing and storage .…”

Section: Resultsmentioning

confidence: 99%

Comparision of Green and Conventional Extraction Methods on Lipid Yield and Fatty Acid Profiles of Fish Species

Özoğul

Uçar

Takadaş

et al. 2018

Euro J Lipid Sci & Tech

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The efficiency of the green extraction methods ultrasound‐assisted extraction (UAE) and microwave‐assisted extraction (MAE), and conventional methods (Soxhlet and Bligh and Dyer), on lipid content and fatty acid profiles of six fish species (Mullus barbatus, red mullet; Upeneus moluccensis, goldband goatfish; Mullus surmuletus, surmullet; Anguilla anguilla, European eel; Pagellus erythrinus, common pandora, and Saurida undosquamis, brushtooth lizardfish) are evaluated. The results of lipid content of fish species show that the Bligh and Dyer method and UAE in general are more efficient than other methods. There are statistical differences in the fatty acid composition of fish oil by four extraction methods (p < 0.05). Saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acid (PUFA) contents of fish species range from 29.51 mg 100 g−1 fish (Soxhlet)–1400 mg 100 g−1 (UAE), 15.52 mg 100 g−1 (UAE)–2237.18 mg 100 g−1 (Bligh and Dyer), and 14.36% (Soxhlet)–646 mg 100 g−1 (Bligh and Dyer), respectively. Generally, Bligh and Dyer give the higher values in surmullet, red mullett, and common pandora in terms of SFA, MUFA, PUFA, and the dominant fatty acids (C16:0, C18:0, C16:1, C18:1n9, C18:1n7, C22:1n9, EPA, DHA), whereas MAE and UAE methods give better results in goldband goldfish and European eel, respectively. Thus, extraction methods affect the lipid yield and fatty acid profiles of extracted oil of different fish species. Practical Application: Total lipid content of seafood is an important parameter used in biochemical, physiological, and nutritional studies. Thus, reliable methods for the quantitative extraction of lipids from this type of food are of critical importance. The effects of green and conventional extraction methods on lipid yield and fatty acid profiles of fish species are compared in order to determine the most effective method for extracting lipid with high yield and high content of PUFA, especially EPA and DHA. The effects of green and conventional extraction methods on lipid yield and fatty acid profiles of fish species are evaluated. The extraction methods affect the lipid yield and fatty acid profiles of extracted oil of fish species. The Bligh and Dyer method and UAE in general are more efficient than other methods. In terms of SFA, MUFA, PUFA, EPA, and DHA; Bligh and Dyer gave the higher values in surmullet, redmullet, and common pandora whereas MAE and UAE methods gave better results in goldband goldfish and European eel, respectively.

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Section: Resultsmentioning

confidence: 99%

Comparision of Green and Conventional Extraction Methods on Lipid Yield and Fatty Acid Profiles of Fish Species

Özoğul

Uçar

Takadaş

et al. 2018

Euro J Lipid Sci & Tech

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“…Fatty acids were extracted from spleen samples using microwave assisted extraction. 41 Fatty acids were extracted using a 4:1 (v/v) ethyl acetate:methanol solution in a Mars 6 microwave system (CEM Corporation, Matthews, NC) with the following heating profile: 1 min ramp to 40 • C, 12 min hold with max power of 400 W. Fatty acyl methyl esters were synthesized using the HCl method. 42 A 680/600S GC-MS (Perkin Elmer, Waltham, MA) equipped with Agilent Technologies (Santa Clara, CA, USA) DB-23 30-m column was used for data acquisition.…”

Section: Fatty Acid Extraction and Gc/ms Lipidomicsmentioning

confidence: 99%

Frontline Science: A reduction in DHA-derived mediators in male obesity contributes toward defects in select B cell subsets and circulating antibody

Crouch

Kosaraju

Guesdon

et al. 2018

Journal of Leukocyte Biology

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Obesity dysregulates B cell populations, which contributes toward poor immunological outcomes. We previously reported that differing B cell subsets are lowered in the bone marrow of obese male mice. Here, we focused on how lipid metabolites synthesized from docosahexaenoic acid (DHA) known as specialized pro‐resolving lipid mediators (SPMs) influence specific B cell populations in obese male mice. Metabololipidomics revealed that splenic SPM precursors 14‐hydroxydocosahexaenoic acid (14‐HDHA), 17‐hydroxydocosahexaenoic acid (17‐HDHA), and downstream protectin DX (PDX) were decreased in obese male C57BL/6J mice. Simultaneous administration of these mediators to obese mice rescued major decrements in bone marrow B cells, modest impairments in the spleen, and circulating IgG2c, which is pro‐inflammatory in obesity. In vitro studies with B cells, flow cytometry experiments with ALOX5−/− mice, and lipidomic analyses revealed the lowering of 14‐HDHA/17‐HDHA/PDX and dysregulation of B cell populations in obesity was driven indirectly via B cell extrinsic mechanisms. Notably, the lowering of lipid mediators was associated with an increase in the abundance of n‐6 polyunsaturated fatty acids, which have a high affinity for SPM‐generating enzymes. Subsequent experiments revealed female obese mice generally maintained the levels of SPM precursors, B cell subsets, and antibody levels. Finally, obese human females had increased circulating plasma cells accompanied by ex vivo B cell TNFα and IL‐10 secretion. Collectively, the data demonstrate that DHA‐derived mediators of the SPM pathway control the number of B cell subsets and pro‐inflammatory antibody levels in obese male but not female mice through a defect that is extrinsic to B cells.

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“…They have been reviewed by Iverson and coworkers [21]. Among them, the use of ultrasound [1,22,23,24], microwaves [16,22,23,25,26], heat [25,27], pressure [27,28] or beads [24,27] were often raised to improve the efficiency of lipid recovery from various tissues such as bacteria [23], yeasts [29] or microalgae [30] (supplementary material). However, all these methods were using solvents of fossil origin issued from petrol.…”

Section: Introductionmentioning

confidence: 99%

“Bligh and Dyer” and Folch Methods for Solid–Liquid–Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents

Breil

Vian

Zemb

et al. 2017

IJMS

213

117

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Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are “gold standards” for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid–liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid–liquid partition—the two steps of routine extraction. For this purpose, we consider similar points in the ternary phase diagrams of water/methanol/chloroform and water/ethanol/ethyl acetate, both in the monophasic mixtures and in the liquid–liquid miscibility gap. Based on high performance thin-layer chromatography (HPTLC) to obtain the distribution of lipids classes, and gas chromatography coupled with a flame ionisation detector (GC/FID) to obtain fatty acid profiles, this greener solvents pair is found to be almost as effective as the classic methanol–chloroform couple in terms of efficiency and selectivity of lipids and non-lipid material. Moreover, using these bio-sourced solvents as an alternative system is shown to be as effective as the classical system in terms of the yield of lipids extracted from microorganism tissues, independently of their apparent hydrophilicity.

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Rapid microwave assisted extraction of meat lipids

Cited by 24 publications

References 43 publications

Comparision of Green and Conventional Extraction Methods on Lipid Yield and Fatty Acid Profiles of Fish Species

Comparision of Green and Conventional Extraction Methods on Lipid Yield and Fatty Acid Profiles of Fish Species

Frontline Science: A reduction in DHA-derived mediators in male obesity contributes toward defects in select B cell subsets and circulating antibody

“Bligh and Dyer” and Folch Methods for Solid–Liquid–Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents

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