Rapid method for the isolation of two purified subfractions of high density lipoproteins by differential dextran sulfate-magnesium chloride precipitation

Burstein, M; Fine, A.; Atger, V.; Wirbel, E; Girard-Globa, Anik

doi:10.1016/0300-9084(89)90090-4

Cited by 46 publications

(34 citation statements)

References 11 publications

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“…2 ) Dextran sulfate/MgCl 2 (Sigma-Aldrich) precipitation. Dextran sulfate (20 l) (5% in water) and 25 l of 2 M MgCl 2 were added to 1 ml serum, incubated for 20 min at room temperature, and then centrifuged at 3,000 g for 10 min ( 26 ). 3 ) Heparin sodium/manganese chloride (MnCl 2 ; Sigma-Aldrich) precipitation.…”

Section: Apob Depletion Methodsmentioning

confidence: 99%

“…apoA-I immunoblotting was performed on serum and apoBdepleted serum fractions in the HDL subspecies range (fractions [20][21][22][23][24][25][26][27][28][29][30]. Ten microliters of each fraction was resolved by SDS-PAGE (4-15%) and transferred to a polyvinylidene difl uoride membrane.…”

Section: Western Blotmentioning

confidence: 99%

“…The phospholipid profi le of untreated serum is shown in Fig. 10 , where the fi rst peak (fractions 3-15) represents HDL-sized particles and the second peak (fractions [25][26][27][28][29][30] represents VLDL/LDL-sized particles (again, using the presence of apoB as a distinguisher). Upon precipitation with PEG, dextran sulfate/MgCl 2 , and heparin sodium/ MnCl 2 , the VLDL/VLDL peak disappeared consistent with apoB depletion.…”

Section: Hdl Functionmentioning

confidence: 99%

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The effects of apolipoprotein B depletion on HDL subspecies composition and function

Davidson

Heink

Sexmith

et al. 2016

Journal of Lipid Research

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Section: Apob Depletion Methodsmentioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

Section: Hdl Functionmentioning

confidence: 99%

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The effects of apolipoprotein B depletion on HDL subspecies composition and function

Davidson

Heink

Sexmith

et al. 2016

Journal of Lipid Research

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“…The sequential ultracentrifugation method described here, with two different steps to discriminate between lighter HDL and heavier HDL (2,6,30), allowed us to obtain a lipoprotein fraction for which ApoA-I represented more than 90% of the total protein. Decreases in the concentration of the protein of around 50% were observed after experimental inflammation.…”

mentioning

confidence: 99%

The Concentration of Apolipoprotein A-I Decreases during Experimentally Induced Acute-Phase Processes in Pigs

et al. 2005

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In this work, apolipoprotein A-I (ApoA-I) was purified from pig sera. The responses of this protein after sterile inflammation and in animals infected with Actinobacillus pleuropneumoniae or Streptococcus suis were investigated. Decreases in the concentrations of ApoA-I, two to five times lower than the initial values, were observed at 2 to 4 days. It is concluded that ApoA-I is a negative acute-phase protein in pigs.

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“…HDL 2 and HDL 3 were separated by precipitation (Burstein, Fine, Atger, Wirbel, & Girard-Globa, 1989) using MgCl 2 and dextran sulfate weight 500,000 (Sigma Chemical Company, France).…”

Section: Isolation Of Serum Lipoproteins Fractionsmentioning

confidence: 99%

Effects of Two Hypocaloric Diets Supplemented With White Lupine or Oats on Lipid Peroxidation, Reverse Cholesterol Transport and Paraoxonase Activity in Obese Rat

Chabane¹,

Louala²,

Boukhari³

et al. 2013

JFR

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The purpose of this study was to compare the effects of two hypocaloric diets supplemented with legume or cereal on hyperglycemia, dyslipidemia, serum HDL 2 and HDL 3 composition, lipid peroxidation, and lecithin-cholesterol acyltransferase (LCAT) and paraoxonase (PON1) activities in obese rats. Obesity was induced by feeding a high-fat-diet during 3 months. At 400 ± 20 g, the obese rats were divided into three groups (n = 8): two groups were submitted for 28 days to a caloric restriction (CR) at 0.9 MJ supplemented with lupine (CR-Lupine group) or oats (CR-Oats group). The third group was fed a caloric restricted diet without supplementation (CR group). At day 28, glycemia was lower (-21%) in the CR-Oats than the CR group. CR-Oats diet reduced total cholesterol content (-13%) when compared to lupine supplementation. In contrast, CR-Lupine diet decreased serum triacylglycerols by 22% and 15% respectively versus CR-Oats and CR diets. Lipoproteins lipid peroxidation was significantly lower in CR-Lupine and CR-Oats when compared to the CR diet. Serum PON1 activity was increased in CR-Lupine (+88%) and CR-Oats (+82%) groups compared to CR group. Furthermore, LCAT activity was enhanced in CR-Lupine (+23% and +26%, respectively) versus CR and CR-Oats groups. In obese rats, caloric restriction supplemented with lupine compared to oats supplementation has beneficial effect on hypertriglyceridemia and improves reverse cholesterol transport by enhancing LCAT activity leading to anti-atherogenic effects. This effect is partially reinforced by the high HDL PON1 activity which protects it from oxidation.

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Rapid method for the isolation of two purified subfractions of high density lipoproteins by differential dextran sulfate-magnesium chloride precipitation

Cited by 46 publications

References 11 publications

The effects of apolipoprotein B depletion on HDL subspecies composition and function

The effects of apolipoprotein B depletion on HDL subspecies composition and function

The Concentration of Apolipoprotein A-I Decreases during Experimentally Induced Acute-Phase Processes in Pigs

Effects of Two Hypocaloric Diets Supplemented With White Lupine or Oats on Lipid Peroxidation, Reverse Cholesterol Transport and Paraoxonase Activity in Obese Rat

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