Rapid method for the analysis of tobacco nicotine alkaloids

Severson, Ray F.; McDuffie, K.L.; Arrendale, Richard F.; Gwynn, G.R.; Chaplin, J. F.; Johnson, Albert W.

doi:10.1016/s0021-9673(00)81179-6

Cited by 63 publications

(33 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prior to chemical analyses, midveins and lamina were separated, dried in an oven at 85 ac, ground to pass a 20-mesh sieve, and placed in a desiccator. Alkaloids were determined by the gas chromatography (GC) method of SEVERSON et al (17). The values for total alkaloids represented a sum of the individual alkaloids (nicotine, nornicotine, anabasine, anatabine) obtained in the GC analyses .…”

Section: Methodsmentioning

confidence: 99%

Effects of Curing and Fertilization on Nitrosamine Formation in Bright and Burley Tobacco

Chortyk

1992

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

View full text Add to dashboard Cite

SUMMARYA Bright and a Burley tobacco were grown at four fertilization rates and each tobacco was then both fluecured and air-cured. Levels of alkaloids and nitrosamines were found to increase with increasing fertilization levels. Levels of alkaloids, N -nitrosonornicotine (NNN), and other tobacco-specific nitrosamines (TSNA) were consistently higher in the Burley tobacco than in the Bright tobacco, regardless of curing method. In comparing the effects of curing, it was found that NNN and total TSNA levels were higher in the midrib than in the lamina of the air-cured samples, while just the opposite was found for the flue-cured samples. Flue-curing Bright tobacco produced three times the level of TSNA v s air-curing the same tobacco. On the other hand, flue-curing Burley tobacco reduced the alkaloids, but greatly increased the TSNA in the lamina. As midribs from the air-cured Burley leaves had three times the TSNA concentration of the lamina, the use of air-cured midribs in tobacco products should be avoided. It was concluded that lower fertilization levels and careful manipulations of curing parameters could lower nitrosamine levels in cured tobacco.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of Curing and Fertilization on Nitrosamine Formation in Bright and Burley Tobacco

Chortyk

1992

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

View full text Add to dashboard Cite

show abstract

“…For comparison purposes an alternative solvent extraction procedure from Health Canada Official Method T-301 [25], based on the work of Severson et al [26] was applied. Briefly, a 25-mg of ground cigarette filler sample was extracted with 1 mL of 0.05 mol/L methanolic KOH solution in the ultrasonic bath for 3 h. A portion of the extract was filtered through a 0.2-μm Nylon syringe filter, diluted with the mobile phase at a ratio 1:25 and analyzed.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Finally, we compared the alkaloid yields obtained from the optimized extraction procedure with well established method using methanolic KOH solution [25,26]. For this comparison, two tobacco filler samples from different brands of cigarettes were used, with a triplicate extraction for each method.…”

Section: Extractionmentioning

confidence: 99%

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Taujenis¹,

Olšauskaitė²,

Padarauskas³

2015

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 µg/g for cotinine, 20 µg/g for anabasine and nornicotine, and 30 µg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

show abstract

“…A rapid glass-capillary gas-chromatographic (GC) method (13) was used for the analysis of nicotine, nornicotine, anabasine, anatabine, and myosmine (and in 1984, 85 and 86 samples, N-acetylnornicotine) present in the Nicotiana species. About 25 mg to 50 mg of the ground laminar sample and 1 m!…”

Section: Analytical Proceduresmentioning

confidence: 99%

Alkaloid Composition of the Nicotiana Species

Sisson

Severson

1990

Contributions to Tobacco &Amp; Nicotine Research

View full text Add to dashboard Cite

SUMMARYPlants from 64 Nicotiana species were sampled in the greenhouse in 1983 and 1984 and from the field in 1985 and 1986 for the purpose of developing a chemical profile of each species. Mature green leaves were harvested at flowering, freeze-dried and ground to pass a 1 mm screen prior to chemical analysis. In this study we report the type and amounts of nicotinoid alkaloids. Alkaloid values were determined by glass -capillary gas chromatography and were averaged over the two years for each environment. Both total alkaloids (mg g-1 dry weight) and the distribution (percentage composition) of nicotine, nornicotine, anabasine, and anatabine were highly correlated between years for greenhouse and field samples. Greenhouse and field data were highly correlated, although total-alkaloid levels were significantly higher from field-grown plants. All of the Nicotiana species tested contained a measurable alkaloid fraction (at least 10 !lg g-1 ) . There was a wide range in total-alkaloid levels with nearly a

show abstract

Rapid method for the analysis of tobacco nicotine alkaloids

Cited by 63 publications

References 10 publications

Effects of Curing and Fertilization on Nitrosamine Formation in Bright and Burley Tobacco

Effects of Curing and Fertilization on Nitrosamine Formation in Bright and Burley Tobacco

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Alkaloid Composition of the Nicotiana Species

Contact Info

Product

Resources

About