Rapid method for obtaining high-quality chromosome banding in the study of hematopoietic neoplasia

Novak, Angelina; Kruskic, Mirna; Ludoski, M.; Jurukovski, Vladimir

doi:10.1016/0165-4608(94)90007-8

Cited by 40 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromosomes were examined with modified Giemsa staining HG-banding technique, which was described previously in detail (Novak et al, 1994). The karyotypes were presented in accordance with the guidelines of the International System for Human Cytogenetic Nomenclature (ISCN) (Shaffer et al, 2009).…”

Section: Patientsmentioning

confidence: 99%

Cytogenetic findings in Serbian patients with Turner’s syndrome stigmata

Djordjević

Jovanović²,

Pavković-Lučić³

et al. 2010

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Cytogenetic findings are reported for 31 female patients with Turner's syndrome. Chromosome studies were made from lymphocyte cultures. Non-mosaicism 45,X was demonstrated in 15 of these patients, whereas only three were apparently mosaic. Eight patients showed nonmosaic and four patients showed mosaic structural aberrations of the X-chromosome. One non-mosaic case displayed a karyotype containing a small marker chromosome. Conventional cytogenetics was supplemented by fluorescence in situ hybridization (FISH) with an X-specific probe to identify the chromosomal origin of the ring and a 1q12-specific DNA probe to identify de novo balanced translocation (1;9) in one patient. To our knowledge, this is the first finding of karyotype 45,X,t(1;9) (cen;cen)/46,X,r(X),t(1;9)(cen;cen) in Turner's syndrome. The same X-specific probe was also used to identify a derivative chromosome in one patient.

show abstract

Section: Patientsmentioning

confidence: 99%

Cytogenetic findings in Serbian patients with Turner’s syndrome stigmata

Djordjević

Jovanović²,

Pavković-Lučić³

et al. 2010

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…For FISH analysis, MNCs were prepared similarly to cytogenetic analysis 12 with slight modifications for adhesion slides (Marienfeld, LaudaKoenigshofen, Germany). MNCs (3-5 ϫ 10 5 ) were treated with 200 L hypotonic solution (0.075 M KCl) for 15 minutes at 37°C, dropped on adhesion slides, and incubated for another 10 minutes at room temperature.…”

Section: Fishmentioning

confidence: 99%

Genome-wide analysis of DNA copy number changes and LOH in CLL using high-density SNP arrays

Pfeifer¹,

Pantić²,

Skatulla³

et al. 2006

Blood

208

184

View full text Add to dashboard Cite

Recurrent genomic aberrations are important prognostic parameters in chronic lymphocytic leukemia (CLL). High-resolution 10k and 50k Affymetrix SNP arrays were evaluated as a diagnostic tool for CLL and revealed chromosomal imbalances in 65.6% and 81.5% of 70 consecutive cases, respectively. Among the prognostically important aberrations, the del13q14 was present in 36 (51.4%), trisomy 12 in 9 (12.8%), del11q22 in 9 (12.8%), and del17p13 in 4 cases (5.7%). A prominent clustering of breakpoints on both sides of the MIRN15A/MIRN16-1 genes indicated the presence of recombination hot spots in the 13q14 region. Patients with a monoallelic del13q14 had slower lymphocyte growth kinetics (P ‫؍‬ .002) than patients with biallelic deletions. In 4 CLL cases with unmutated VH genes, a common minimal 3.5-Mb gain of 2p16 spanning the REL and BCL11A oncogenes was identified, implicating these genes in the pathogenesis of CLL. Twenty-four large (> 10 Mb) copy-neutral regions with loss of heterozygosity were identified in 14 cases.

show abstract

“…Cytogenetic analysis has been performed by the use of HG bands. 6 Apoptosis or 'preprogrammed cell death' is a normal mechTo avoid decalcination and cell deformation during the anism responsible for cell and tissue homeostasis. It has very preparation process, we applied a modified technique for use specific molecular changes and morphological patterns and of bone marrow particles.…”

Section: Transformation Three Patients (10%) Had Chronic Myelomono-mentioning

confidence: 99%

Incidence and role of apoptosis in myelodysplastic syndrome: morphological and ultrastructural assessment

Ad¹,

Dp²,

Jankovic³

et al. 1997

Leukemia

View full text Add to dashboard Cite

show abstract

Rapid method for obtaining high-quality chromosome banding in the study of hematopoietic neoplasia

Cited by 40 publications

References 13 publications

Cytogenetic findings in Serbian patients with Turner’s syndrome stigmata

Cytogenetic findings in Serbian patients with Turner’s syndrome stigmata

Genome-wide analysis of DNA copy number changes and LOH in CLL using high-density SNP arrays

Incidence and role of apoptosis in myelodysplastic syndrome: morphological and ultrastructural assessment

Contact Info

Product

Resources

About