Rapid Method for Isolation of PCR Amplifiable Genomic DNA of &amp;lt;i&amp;gt;Ralstonia solanacearum&amp;lt;/i&amp;gt; Infested in Potato Tubers

Grover, Alka; Chakrabarti, Swarup K.; Azmi, Wamik; Khurana, S. M. P.

doi:10.4236/aim.2012.24056

Cited by 9 publications

(8 citation statements)

References 18 publications

(24 reference statements)

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“…In different utilization pattern of various sugars; pathogenic variability and virulence pattern of R. solanacearum belonging to Biovar 1 and Biovar 3 could be due to genetic variability among different strains of pathogen [13].…”

Section: Profiling Of R Solanacearum Isolates Into Biovarsmentioning

confidence: 99%

“…Two loopfuls of bacterium from 48hr old colonies grown on Kelman's TZC Agar was transferred to 5 mL of sterile double distilled water in screw capped vials according to procedure by Grover, et al [13]. They were stored under refrigeration at 20 o C for maintenance of virulence.…”

Section: Preservation Of Ralstonia Solanacearummentioning

confidence: 99%

“…Biovars of R. solanacearum strains were determined by standard procedure according to Grover, et al [13]. The following basal medium was used for biovar identification: NH4H2PO4, 1.0 g; KCl, 0.2 g; MgSO4.7H2O, 0.2 g; Peptone, 1.0 g; 1% (wv -1 ) aqueous solution of bromothymol blue, 0.3 ml; agar, 1.5 g; distilled water, 1litre.…”

Section: Profiling Of R Solanacearum Isolates Into Biovarsmentioning

confidence: 99%

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Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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Article HistoryRalstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5-1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants.

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Section: Profiling Of R Solanacearum Isolates Into Biovarsmentioning

confidence: 99%

Section: Preservation Of Ralstonia Solanacearummentioning

confidence: 99%

Section: Profiling Of R Solanacearum Isolates Into Biovarsmentioning

confidence: 99%

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Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

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“…Genomic DNA was extracted from 22 pathogenic Fusarium isolates (as determined by the Koch's postulates) using CTAB procedure (Grover et al, 2012). These 22 pathogenic isolates of Fusarium represented the two wilt-infected crops and the four districts.…”

Section: Dna Extractionmentioning

confidence: 99%

Molecular identification of <i>Fusarium</i> spp. from wilt-infected tomato and brinjal plants in selected regions of Sri Lanka and endophytic bacteria as a potential option for disease management

Weeraratne

Costa

2018

Trop. Agric. Res.

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Fusarium wilt caused by Fusarium spp. is an economically important fungal disease of tomato and brinjal production areas in Sri Lanka. The study was carried out to identify Fusarium isolates infecting tomato and brinjal, and endophytic antagonists bacteria against Fusarium wilt pathogen. The infected tomato and brinjal samples were collected from farmers' fields in Matale, Kandy, Nuwara Eliya and Badulla districts and PCR was conducted using primers specific for species, races and formae speciales. Eight, nine and five isolates were identified as Fusarium solani, Fusarium oxysporum f. sp. lycopersici race 1 and Fusarium oxysporum f. sp. radicis-lycopersici, respectively from the wilt-infected tomato and brinjal collected from the four districts. Thirty endophytic bacterial isolates were isolated from healthy tomato and brinjal stems were antagonistic against Fusarium oxysporum f. sp. lycopersici and Fusarium solani. Molecular identification revealed that Pseudomonas geniculata strain ICPH-14, Pseudomonas sp. strain SB 904 (E7), Delftia tsuruhatensis strain MTQ 1, Stenotropomonas maltophilia strain ATCC 13637, Stenotropomonas pavanii strain ICB 89, and Bacillus velezensis strain C19 were among the potential endophytic antagonists.

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“…As a result, clear bands were obtained for the given DNA sample. Grover et al (2012) also used phenol chloroform method and extracted DNA.…”

Section: Dna Isolation and Agarose Gel Electrophoresismentioning

confidence: 99%

Detection of hdc Gene from Histamine Producing Bacillus subtillis and Serratia marcescens

Laxmi¹,

Munaliga²,

Babu³

et al. 2017

Int J Appl Sci Biotechnol

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In the present study, two histamine producing bacteria were used to detect the presence of hdc gene using PCR. The histamine production was confirmed by using Soyabean Casein Digest Agar (Tryptone Soya Agar). The DNA was isolated using Phenol: chloroform method. PCR was carried out using hdc gene and the amplified product was confirmed (657bps and 668 bps respectively). Further, the DNA and the PCR product were transformed into E.coli and the gene transformation was confirmed.

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Rapid Method for Isolation of PCR Amplifiable Genomic DNA of <i>Ralstonia solanacearum</i> Infested in Potato Tubers

Cited by 9 publications

References 18 publications

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Molecular identification of <i>Fusarium</i> spp. from wilt-infected tomato and brinjal plants in selected regions of Sri Lanka and endophytic bacteria as a potential option for disease management

Detection of hdc Gene from Histamine Producing Bacillus subtillis and Serratia marcescens

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