Rapid method for estimating microsomal oxidase activity in the housefly with carbon-14-labeled parathion as substrate

Rhee, K.S.; Plapp, Frederick W.

doi:10.1021/jf60192a042

Cited by 7 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measurement of in Vitro NADPH-Dependent Parathion Metabolism. The procedure of Rhee and Plapp (1974) for estimating microsomal oxidase activity was used with 1 nmol of [14C]ethyl parathion (= parathion) in 20 µ < of 2-propanol as substrate. Competitive inhibition of parathion metabolism was measured by adding 1, 10, or 100 nmol of [12C] parathion, methyl parathion, methamidophos, or acephate to reaction flasks along with [14C] parathion before adding fly homogenate.…”

Section: Methodsmentioning

confidence: 99%

“…The incubation was carried out for 15 min and terminated by the addition of 1 mL chloroform. The detection of radioactive water-soluble metabolites followed the procedure of Rhee and Plapp (1974).…”

Section: Methodsmentioning

confidence: 99%

“…The procedure of Rhee and Plapp (1974) for estimating microsomal oxidase activity was used with 1 nmol of [14C]ethyl parathion (= parathion) in 20 pL of 2-propanol as substrate. The procedure of Rhee and Plapp (1974) for estimating microsomal oxidase activity was used with 1 nmol of [14C]ethyl parathion (= parathion) in 20 pL of 2-propanol as substrate.…”

Section: Strains Of Housefliesmentioning

confidence: 99%

“…The detection of radioactive water-soluble metabolites followed the procedure of Rhee and Plapp (1974). The detection of radioactive water-soluble metabolites followed the procedure of Rhee and Plapp (1974).…”

Section: Strains Of Housefliesmentioning

confidence: 99%

See 3 more Smart Citations

Mechanisms by which methamidophos and acephate circumvent resistance to organophosphate insecticides in the housefly

Suksayretrup¹,

Plapp²

1977

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Finally, one of the minor products (3.9%) was identified as epoxy precocene I1 based on its R, values in 4:l ether-hexane (0.85) and 93:7 benzeneethyl acetate (0.41), its GC retelition time, and its mass spectrum which was identical with 3,4-epoxy-6,7-dimethoxy-2,2-dimethylchtoman. At this time, it is not known whether epoxy precocene I1 serves as an ilitermediate in the formation of precocene I1 diol, but it is certainly a possible metabolic pathway.All of the synthetic metabolites were bioassayed for antijuvenile hormone activity using the milkweed bug as the test organism (Bowers et al., 1976). However, none of the compounds were active. ACKNOWLEDGMENT We thank C. W. Hessney for technical assistance.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%