Rapid method for detection of Vibrio cholerae from drinking water with nanomaterials enhancing electrochemical biosensor

Savas, Sumeyra; Saricam, Melike

doi:10.1016/j.mimet.2023.106862

Cited by 2 publications

(2 citation statements)

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“…These constants were derived from fitting bacterial inactivation curves with Equations ( 3) and (4). In addition, k radicals were calculated via the decreased levels after quenching radicals by TBA, as shown in Equation (5).…”

Section: Application Of Quenchers With Radicalsmentioning

confidence: 99%

“…Bacterial contamination in drinking water has consistently remained a critical concern for public health. Pathogens such as Escherichia coli [1,2], Salmonella [3,4], Vibrio cholerae [5], and Legionella pneumophila [6] have attracted widespread attention, prompting the development of monitoring and prevention strategies. However, in recent years, threats to drinking water safety have expanded beyond traditional pathogens to include a broader range of environmental microbes, with Pseudomonas aeruginosa (P. aeruginosa) being a typical example [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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A Combination of UV and Disinfectant for Inactivating Viable but Nonculturable State Pseudomonas aeruginosa: Efficiency and Mechanisms

Zhao,

Zhu,

Tao

et al. 2024

Water

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Conventional disinfection techniques, relying on a single disinfection step, often fail to directly eliminate microorganisms, instead causing them to enter a viable but nonculturable (VBNC) state. However, microorganisms in the VBNC state retain metabolic activity and can reactivate under suitable conditions, representing a “hidden source of contamination” that threatens drinking water safety. This study fundamentally assessed the feasibility of combined disinfection methods by integrating UV254 with disinfectant (NaClO, PAA, and PDS) for inactivating Pseudomonas aeruginosa (P. aeruginosa), an opportunistic pathogen that has been widely detected in water supply systems. The number of culturable cells was determined using the heterotrophic plate counting (HPC) method, and the number of VBNC cells was quantified using our recently developed qPCR approach. Quantitative analyses showed that combined disinfection methods can effectively reduce both culturable and VBNC cells by several orders of magnitude compared to a single disinfection step. Notably, VBNC P. aeruginosa, after 30 min of UV/NaCIO treatment, was below the detection limit (3.191 log CFU/mL) of PMA-qPCR. The reactivation experiment also confirmed that VBNC P. aeruginosa did not reactivate for 16 h after 30 min of UV/NaClO treatment under controlled laboratory conditions. The higher disinfection capacity of combined methods can be attributed to the generation of reactive radicals. This study highlighted combined disinfection as a promising approach for the inactivation of bacteria in the VBNC state, yet further studies are needed before an application can be considered for minimizing VBNC reactivation in city utility water processing or high-risk building water distribution systems.

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Section: Application Of Quenchers With Radicalsmentioning

confidence: 99%