Rapid metabolic profiling of developing Pseudomonas aeruginosa biofilms by high-resolution mass spectrometry fingerprinting

Borgos, Sven Even F.; Skjåstad, Rune; Tøndervik, Anne; Aas, Marianne; Aasen, Inga Marie; Brunsvik, Anders; Holten, Torunn; Iversen, Ole‐Jan; Ahlén, Catrine; Zahlsen, Kolbjørn

doi:10.1007/s13213-014-0930-z

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…As the Bcc and P. aeruginosa share similar biology, the studies on P. aeruginosa could essentially guide the study of metabolic profile underlying the virulence and antibiotic resistance of B. cepacia complex. Using clinical isolates and mutant libraries researchers have been able to characterize the metabolites involved in metabolic adaptation, biofilm formation and antibiotic resistance of P. aeruginosa in CF patients ( Behrends et al, 2013a , b ; Borgos et al, 2014 ). In one study the research group of Behrends assessed the metabolic alteration of P. aeruginosa during chronic infection using NMR spectroscopy ( Behrends et al, 2013b ).…”

Section: Potential Of Metabolomics In the Study Of Bcc Pathogenesismentioning

confidence: 99%

“…One remarkable finding of this NMR study is that discharge of excessive amounts of gluconate by a number of mutant strains correlates with reduced susceptibility to antibiotics tobramycin, ciprofloxacin, aztreonam and imipenem, indicating a direct relevance of gluconate accumulation with antibiotic resistance of many pathogenic P. aeruginosa strains ( Behrends et al, 2013a ). Recently another research group has applied high resolution MS fingerprinting to study the metabolic profile underlying P. aeruginosa biofilm formation during opportunistic infection ( Borgos et al, 2014 ). Extracellular biofilm compounds analyzed from in vitro cultures of four different P. aeruginosa strains at different time points have revealed strain and time dependent changes in the metabolic profiles.…”

Section: Potential Of Metabolomics In the Study Of Bcc Pathogenesismentioning

confidence: 99%

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Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance

2015

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The Burkholderia cepacia complex (Bcc) is a collection of closely related, genetically distinct, ecologically diverse species known to cause life-threatening infections in cystic fibrosis (CF) patients. By virtue of a flexible genomic structure and diverse metabolic activity, Bcc bacteria employ a wide array of virulence factors for pathogenesis in CF patients and have developed resistance to most of the commonly used antibiotics. However, the mechanism of pathogenesis and antibiotic resistance is still not fully understood. This mini review discusses the established and potential virulence determinants of Bcc and some of the contemporary strategies including transcriptomics and proteomics used to identify these traits. We also propose the application of metabolic profiling, a cost-effective modern-day approach to achieve new insights.

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Section: Potential Of Metabolomics In the Study Of Bcc Pathogenesismentioning

confidence: 99%

Section: Potential Of Metabolomics In the Study Of Bcc Pathogenesismentioning

confidence: 99%

Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance

2015

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“…Nowadays, liquid chromatography-mass spectrometry (LC–MS)-based metabolomics is considered as a highly sensitive and comprehensive analytical approach that is widely used in identifying potential biomarkers that can be used to monitor the efficacy of drug therapies (Zhang et al 2020 ). Metabolomics was used to investigate the underlying mechanism of various QSIs such as azithromycin (Chen et al 2017 ; Phelan et al 2015 ), and to effectively differentiate various P. aeruginosa strains (Borgos et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Micafungin effect on Pseudomonas aeruginosa metabolome, virulence and biofilm: potential quorum sensing inhibitor

et al. 2023

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The prevalence of antibiotic resistance in Pseudomonas aeruginosa places a heavy burden on the health care sectors urging the need to find alternative, non-antibiotic strategies. The interference with the P. aeruginosa quorum sensing (QS) system represents a promising alternative strategy to attenuate the bacterial virulency and its ability to form biofilms. Micafungin has been reported to impede the pseudomonal biofilm formation. However, the influences of micafungin on the biochemical composition and metabolites levels of P. aeruginosa have not been explored. In this study, the effect of micafungin (100 µg/mL) on the virulence factors, QS signal molecules and the metabolome of P. aeruginosa was studied using exofactor assay and mass spectrometry-based metabolomics approaches. Furthermore, confocal laser scanning microscopy (CLSM) using the fluorescent dyes ConA-FITC and SYPRO® Ruby was used to visualize micafungin disturbing effects on the pseudomonal glycocalyx and protein biofilm-constituents, respectively. Our findings showed that micafungin significantly decreased the production of various QS-controlled virulence factors (pyocyanin, pyoverdine, pyochelin and rhamnolipid), along with a dysregulation in the level of various metabolites involved in QS system, lysine degradation, tryptophan biosynthesis, TCA cycle, and biotin metabolism. In addition, the CLSM examination showed an altered matrix distribution. The presented findings highlight the promising role of micafungin as a potential quorum sensing inhibitor (QSI) and anti-biofilm agent to attenuate P. aeruginosa pathogenicity. In addition, they point to the promising role of metabolomics study in investigating the altered biochemical pathways in P. aeruginosa.

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“…Metabolomics has been used to gain insight into the molecular mechanism of biofilm formation in other bacterial pathogens ( Yeom et al., 2013 ; Stipetic et al., 2016 ; Favre et al., 2018 ; Lu et al., 2019 ; Rieusset et al., 2020 ; Tang et al., 2021 ), but a comprehensive and quantitative analysis of metabolic changes involved in biofilm formation in P. aeruginosa is still missing ( Gjersing et al., 2007 ; Zhang and Powers, 2012 ; Borgos et al., 2015 ). Detailed elucidation of major shifts in metabolic pathways in planktonic versus biofilm phenotypes has the potential to identify mechanisms of metabolic regulation, new targets for prevention and mitigation, and specific metabolic signatures for diagnosis of biofilms.…”

Section: Introductionmentioning

confidence: 99%

Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics

Leggett

Sindeldecker

et al. 2022

Front. Cell. Infect. Microbiol.

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There is a critical need to accurately diagnose, prevent, and treat biofilms in humans. The biofilm forming P. aeruginosa bacteria can cause acute and chronic infections, which are difficult to treat due to their ability to evade host defenses along with an inherent antibiotic-tolerance. Using an untargeted NMR-based metabolomics approach, we identified statistically significant differences in 52 metabolites between P. aeruginosa grown in the planktonic and lawn biofilm states. Among them, the metabolites of the cadaverine branch of the lysine degradation pathway were systematically decreased in biofilm. Exogenous supplementation of cadaverine caused significantly increased planktonic growth, decreased biofilm accumulation by 49% and led to altered biofilm morphology, converting to a pellicle biofilm at the air-liquid interface. Our findings show how metabolic pathway differences directly affect the growth mode in P. aeruginosa and could support interventional strategies to control biofilm formation.

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Rapid metabolic profiling of developing Pseudomonas aeruginosa biofilms by high-resolution mass spectrometry fingerprinting

Cited by 5 publications

References 17 publications

Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance

Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance

Micafungin effect on Pseudomonas aeruginosa metabolome, virulence and biofilm: potential quorum sensing inhibitor

Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics

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