Rapid Measurement of Residual Kanamycin Using Highly Specific Biomimetic Recognition Paper-Based Chip

Abstract

The development of highly specific biomimetic recognition material is a challenge for rapid detection of harmful residues in foodstuff. In this study, a paper-based boronate affinity metal–organic framework/molecularly imprinted polymer microfluidic chip (FZS-BA@MIP) was constructed based on the in situ construction strategy, which was also designed as a highly specific biomimetic recognition module. Here, the homogeneous zeolitic imidazole framework-8 (ZIF-8) membrane served as a great scaffold and enrichment layer. Besides, the recognition layer of MIP was prepared based on a highly oriented boronate affinity surface imprinting strategy. With the aid of the liquid flow channel, the highly specific enrichment and visual detection for antibiotic residues like kanamycin in actual products were achieved on the paper chip module of an integrated lateral flow platform. The whole analysis process could be accomplished within 30 min. In brief, this study offered a new integrated biomimetic recognition platform for visually detecting harmful veterinary residues containing cis-diols, which demonstrated promising commercial value in point-of-care testing of foodborne hazardous compounds.

Biosensors with Boronic Acid-Based Materials as the Recognition Elements and Signal Labels

“…To avoid nonspecific binding and improve selectivity, many efforts have been devoted to the fabrication of sandwich-type biosensors, in which cis -diol-containing targets were captured by a recognition element-modified electrode and then recognized with boronic acid-modified signal labels, providing a detectable electrochemical signal. For instance, boronate-affinity controllable-oriented imprinting can be utilized for surface printing and molecular immobilization on the boronic acid-modified electrode [ 101 ]. In this part, we mainly summarized the progress of electrochemical biosensors with boronic acid-based electroactive molecules and nanomaterials as recognition elements and signal labels to produce electrochemical responses ( Table 1 ).…”