Rapid measurement of 13C-enrichment of acetic, propionic and butyric acids in plasma with solid phase microextraction coupled to gas chromatography–mass spectrometry

Moreau, Noëlle M.; Delépée, Raphaël; Maume, Daniel; Bizec, Bruno Le; Nguyen, Patrick; Champ, Martine; Martin, Lucile; Dumon, H.

doi:10.1016/j.aca.2004.02.054

Cited by 12 publications

(9 citation statements)

References 19 publications

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“…33 Our results showed clearly that the LS mode allows a better adsorption of SCVAs in human plasma. These results are also in agreement with those of Moreau et al in rat plasma 24 and Lattuati-Derieux et al in natural aged books. 27 This current method has been validated for the measurement of low SCVA 13 C-enrichments with tests of linearity, repeatability (intra, inter-analysis) and within-day reproducibility in standard solutions and in human plasma.…”

Section: Discussionsupporting

confidence: 95%

“…The carboxen/polydimethylsiloxane fiber was chosen because it is known to perform well in the extraction of low molecular weight volatile fatty acids (VFAs) from aqueous solutions, 28 waste waters, 26 old books 27 or from rat plasma samples. 24 There have been many reports on the use of SPME in combination with GC/MS but few data has been reported on the use of SPME for isotopic measurement by GC/C/IRMS. 17,29 This paper describes a two-step method combining SPME with GC/C/IRMS to analyze 13 C-acetate and 13 C-butyrate, at low isotopic enrichment (<0.5 MPE), within the physiological concentrations measured in human plasma.…”

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confidence: 99%

“…SPME is based on the equilibrium partitioning of organic compounds between an aqueous sample phase and the organic polymer extraction phase. 17 This technique has been extensively applied for the quantitative analysis of SCVAs from a variety of foods, [18][19][20][21] waste waters, 22 various clinical exudates, 23 and from rat plasma 24 in headspace 25,26 or by immersion of the fiber 27 in the aqueous sample. Different organic coatings with differing polarity on SPME fibers were tested to analyze the SCVAs.…”

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Solid‐phase microextraction method for carbon isotopic analysis of volatile carboxylic acids in human plasma by gas chromatography/combustion/isotope ratio mass spectrometry

Ferchaud‐Roucher

Albert

Champ³

et al. 2006

Rapid Comm Mass Spectrometry

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A new analytical method is described for the determination of the physiological concentration and low-level enrichment of (13)C-short-chain volatile organic acids (SCVAs) (e.g. (13)C-acetate and (13)C-butyrate) in human plasma. This two-step method involves solid-phase microextraction (SPME) coupled to gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) without any organic solvents or derivatizing agents. Two SCVA extraction methods were compared using a carboxen/polydimethylsiloxane fiber: headspace sampling (HS) and liquid sampling (LS) SPME. The influences of extraction temperature and time were tested to optimize the adsorption of SCVAs onto the fiber. The comparison of the peak area responses of the acids in the two adsorption methods showed better sensitivity in the human physiological concentration range in the LS mode than in the HS mode. The accuracy of isotopic enrichment measurement was determined using plasma spiked with (13)C-acetate and (13)C-butyrate solution from 0 to 1 mol percent excess (MPE). The linearity and repeatability (RSD < 5%) were measured in LS mode. Plasma SCVA concentrations were also determined relative to 3-methylvalerate (internal standard). Linearity and repeatability were observed from 0 to 400 microM for acetate, from 0 to 20 microM for propionate, and from 0 to 10 microM for butyrate. This method was also used to determine plasma acetate production obtained from lactulose (an undigestible disaccharide) fermentation in one healthy volunteer over 3 h. The acetate concentration increased twofold, 2 h after oral lactulose intake. These results are in agreement with the data obtained by GC/MS in healthy volunteers and obese adults following a lactulose intake by using higher amounts of labelled tracers.SPME coupled with GC/C/IRMS can be used to analyze (13)C-SCVAs at low enrichment (<0.5 MPE) within the physiological concentration measured in human plasma.

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confidence: 95%

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confidence: 99%

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confidence: 99%

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Solid‐phase microextraction method for carbon isotopic analysis of volatile carboxylic acids in human plasma by gas chromatography/combustion/isotope ratio mass spectrometry

Ferchaud‐Roucher

Albert

Champ³

et al. 2006

Rapid Comm Mass Spectrometry

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“…The more common treatments are an extraction with ion exchange resins [22,23] or with SPE cartridges [24]. Other pretreatment procedures that have been employed for these acids are solvent extraction [13,25], solvent extraction with derivatization [26 -28], direct solid phase microextraction [9,15,29], headspace solid phase microextraction [8,10], online dialysis procedure [30], supported liquid membrane extraction [31], hollow fibre (HF) protected liquid phase microextration [32] and vacuum distillation [33].…”

Section: Introductionmentioning

confidence: 99%

“…Other techniques for analysing SCFAs include stable isotope tracer technique [14,15] after direct solid phase microextraction and CE [16]. HPLC was applied for determination of SCFAs more than other methods [17 -19].…”

Section: Introductionmentioning

confidence: 99%

Liquid–gas–liquid technique for microextraction and preconcentration of short chain fatty acids from aqueous samples

Farajzadeh¹,

Assadi

2009

J of Separation Science

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In this study, an organic solvent-free microextraction technique termed liquid-gas-liquid microextraction (LGLME) was applied for cleanup and preconcentration of volatile short chain fatty acids in beverages and dairy products. Various parameters affecting the extraction efficiency such as extraction time, stirring speed, sodium chloride content, concentration and volume of donor and acceptor phases, and extraction temperature were studied. Repeatability (RSD, 4.2-8.5%), correlation coefficients (0.998-0.999), LODs (10-20 microg/L) and enrichment factors (152-249) were also investigated. Recoveries were achieved in the range of 90-102% in different matrices. The presented method was applied for the analysis of target analytes in some samples such as grape juice, vinegar and dairy products.

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Current literature in mass spectrometry

2005

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 12th. Jan. 2005)

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Rapid measurement of 13C-enrichment of acetic, propionic and butyric acids in plasma with solid phase microextraction coupled to gas chromatography–mass spectrometry

Cited by 12 publications

References 19 publications

Solid‐phase microextraction method for carbon isotopic analysis of volatile carboxylic acids in human plasma by gas chromatography/combustion/isotope ratio mass spectrometry

Solid‐phase microextraction method for carbon isotopic analysis of volatile carboxylic acids in human plasma by gas chromatography/combustion/isotope ratio mass spectrometry

Liquid–gas–liquid technique for microextraction and preconcentration of short chain fatty acids from aqueous samples

Current literature in mass spectrometry

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