Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes

Wan, Yuan; Cheng, Gong; Liu, Xin; Hao, Si Jie; Nisic, Merisa; Zhu, Chuan Dong; Xia, Yiqiu; Li, Wen Qing; Wang, Zhi Gang; Zhang, Wen Long; Rice, Stephen L.; Sebastian, Aswathy; Albert, István; Belani, Chandra P.; Zheng, Shan

doi:10.1038/s41551-017-0058

Cited by 207 publications

(176 citation statements)

References 66 publications

(66 reference statements)

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“…The advantage of the immunological isolation technique is the associated high specificity due to the use of the antibody, although this high selectivity and specificity can result in a low exosome yield compared with physical separation‐based methods. More recently, another immunological method was reported . A lipid nanoprobe system was used to isolate exosomes from serum‐free cell‐culture supernatant and blood plasma.…”

Section: Isolation Of Exosomesmentioning

confidence: 99%

Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles

Boriachek

Islam

Möller

et al. 2017

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346

313

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Exosomes are nanoscale (≈30-150 nm) extracellular vesicles of endocytic origin that are shed by most types of cells and circulate in bodily fluids. Exosomes carry a specific composition of proteins, lipids, RNA, and DNA and can work as cargo to transfer this information to recipient cells. Recent studies on exosomes have shown that they play an important role in various biological processes, such as intercellular signaling, coagulation, inflammation, and cellular homeostasis. These functional roles are attributed to their ability to transfer RNA, proteins, enzymes, and lipids, thereby affecting the physiological and pathological conditions in various diseases, including cancer and neurodegenerative, infectious, and autoimmune diseases (e.g., cancer initiation, progression, and metastasis). Due to these unique characteristics, exosomes are considered promising biomarkers for the diagnosis and prognosis of various diseases via noninvasive or minimally invasive procedures. Over the last decade, a plethora of methodologies have been developed for analyzing disease-specific exosomes using optical and nonoptical tools. Here, the major biological functions, significance, and potential role of exosomes as biomarkers and therapeutics are discussed. Furthermore, an overview of the most commonly used techniques for exosome analysis, highlighting the major technical challenges and limitations of existing techniques, is presented.

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Section: Isolation Of Exosomesmentioning

confidence: 99%

Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles

Boriachek

Islam

Möller

et al. 2017

Small

346

313

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“…The NanoFlare system was combined with flow cytometry analysis and was applied for the detection and molecular analysis of live CTCs in breast cancer . Zheng and co‐workers developed a lipid nanoprobe (LNP) system in which the lipid bilayer of EVs was decorated with biotin‐tagged 1,2‐distearoyl‐ sn ‐glycero‐3‐phosphethanolamine–poly(ethylene glycol) (DSPE–PEG) for the collection by NeutrAvidin (NA)‐coated magnetic sub‐micrometer particles (MMPs), and utilized this system for the detection and the downstream analysis of EGFR and KRAS mutations in the plasma from nonsmall‐cell lung cancer patients …”

Section: Advances and Challenges Of Nanotechnology‐based Liquid Biopsymentioning

confidence: 99%

Emerging Nanotechnologies for Liquid Biopsy: The Detection of Circulating Tumor Cells and Extracellular Vesicles

Li¹,

Wang²,

Zhao³

et al. 2018

Advanced Materials

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Liquid biopsy enables noninvasive and dynamic analysis of molecular or cellular biomarkers, and therefore holds great potential for the diagnosis, prognosis, monitoring of disease progress and treatment efficacy, understanding of disease mechanisms, and identification of therapeutic targets for drug development. In this review, the recent progress in nanomaterials, nanostructures, nanodevices, and nanosensors for liquid biopsy is summarized, with a focus on the detection and molecular characterization of circulating tumor cells (CTCs) and extracellular vesicles (EVs). The developments and advances of nanomaterials and nanostructures in enhancing the sensitivity, specificity, and purity for the detection of CTCs and EVs are discussed. Sensing techniques for signal transduction and amplification as well as visualization strategies are also discussed. New technologies for the reversible release of the isolated CTCs and EVs and for single‐CTC/EV analysis are summarized. Emerging microfluidic platforms for the integral on‐chip isolation, detection, and molecular analysis are also included. The opportunities, challenges, and prospects of these innovative materials and technologies, especially with regard to their feasibility in clinical applications, are discussed. The applications of nanotechnology‐based liquid biopsy will bring new insight into the clinical practice in monitoring and treatment of tumor and other significant diseases.

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“…For instance, click reaction was used to introduce dibenzocyclooctyne‐modified antibody onto the azide prefunctionalized cell membrane . Different from the chemical modification routes, using the lipid molecules (e.g., biotinylated lipid molecules) as anchors which can insert into cell membrane due to the hydrophobic interaction, would pave a more convenient way to bridge further functional components, likely the CTCs recognition antibodies.…”

Section: Introductionmentioning

confidence: 99%

Leukocyte‐Repelling Biomimetic Immunomagnetic Nanoplatform for High‐Performance Circulating Tumor Cells Isolation

Zhou

Luo

Kang

et al. 2019

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Downstream studies of circulating tumor cells (CTCs), which may provide indicative evaluation information for therapeutic efficacy, cancer metastases, and cancer prognosis, are seriously hindered by the poor purity of enriched CTCs as large amounts of interfering leukocytes still nonspecifically bind to the isolation platform. In this work, biomimetic immunomagnetic nanoparticles (BIMNs) with the following features are designed: i) the leukocyte membrane camouflage, which could greatly reduce homologous leukocyte interaction and actualize high‐purity CTCs isolation, is easily extracted by graphene nanosheets; ii) facile antibody conjugation can be achieved through the “insertion” of biotinylated lipid molecules into leukocyte‐membrane‐coated nanoparticles and streptavidin conjunction; iii) layer‐by‐layer assembly techniques could integrate high‐magnetization Fe3O4 nanoparticles and graphene nanosheets efficiently. Consequently, the resulting BIMNs achieve a capture efficiency above 85.0% and CTCs purity higher than 94.4% from 1 mL blood with 20–200 CTCs after 2 min incubation. Besides, 98.0% of the isolated CTCs remain viable and can be directly cultured in vitro. Moreover, application of the BIMNs to cancer patients' peripheral blood shows good reproducibility (mean relative standard deviation 8.7 ± 5.6%). All results above suggest that the novel biomimetic nanoplatform may serve as a promising tool for CTCs enrichment and detection from clinical samples.

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Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes

Cited by 207 publications

References 66 publications

Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles

Biological Functions and Current Advances in Isolation and Detection Strategies for Exosome Nanovesicles

Emerging Nanotechnologies for Liquid Biopsy: The Detection of Circulating Tumor Cells and Extracellular Vesicles

Leukocyte‐Repelling Biomimetic Immunomagnetic Nanoplatform for High‐Performance Circulating Tumor Cells Isolation

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