Rapid magnetic heating treatment by highly charged maghemite nanoparticles on Wistar rats exocranial glioma tumors at microliter volume

Abstract

One of the most significant challenges implementing colloidal magnetic nanoparticles in medicine is the efficient heating of microliter quantities by applying a low frequency alternating magnetic field. The ultimate goal is to accomplish nonsurgically the treatment of millimeter size tumors. Here, we demonstrate the synthesis, characterization, and the in vitro as well as in vivo efficiency of a dextran coated maghemite (gamma-Fe(2)O(3)) ferrofluid with an exceptional response to magnetic heating. The difference to previous synthetic attempts is the high charge of the dextran coating, which according to our study maintains the colloidal stability and good dispersion of the ferrofluid during the magnetic heating stage. Specifically, in vitro 2 mul of the ferrofluid gives an outstanding temperature rise of 33 degrees C within 10 min, while in vivo treatment, by infusing 150 mul of the ferrofluid in animal model (rat) glioma tumors, causes an impressive cancer tissue dissolution.

Magnetic hyperthermia therapy for the treatment of glioblastoma: a review of the therapy’s history, efficacy and application in humans

“…The efficiency of MHT after direct infusion of maghemite NPs was demonstrated in an extracranial rat glioma model [105]. C6 glioma cells were extracranially implanted into rats and allowed to grow for 14 days before inoculation of the maghemite MNPs.…”

Autoresonant switching of the magnetization in single-domain nanoparticles: Two-level theory

“…The theoretical understanding of their behavior is of paramount importance for future applications in a wide variety of areas, ranging from magnetic data storing to waste water decontamination [2] and medical diagnostics and treatments [3][4][5]. In particular, the ability to precisely control the magnetization dynamics constitutes a major advantage, or even, in some cases, an essential requirement [6][7][8][9][10].…”

Design and Application of Magnetic-Based Theranostic Nanoparticle Systems

“…An optimum nanoparticle size between 10 to 100 nm prevents the removal of nanoparticles from circulation and enables them to pass through small capillaries. The size and shape of the particles can be manipulated by surfactant concentrations and types [93], while uniform sizes can be maintained by stirring the ferrofluid during preparation under constant temperature [94]. Further, a surface charge (zeta potential) of 10 to 30 mV or −10 to −30 mV is optimum to achieve a stable nanoparticle suspension with minimal aggregation [95].…”