Rapid liquid chromatography tandem mass spectrometry method for targeted quantitation of human performance metabolites in saliva

McBride, Ethan M.; Lawrence, Richard J.; McGee, Kirstin; Mach, Phillip M.; Demond, Paul S.; Busch, Michael W.; Ramsay, John W.; Hussey, Erika; Glaros, Trevor; Dhummakupt, Elizabeth S.

doi:10.1016/j.chroma.2019.04.071

Cited by 15 publications

(14 citation statements)

References 46 publications

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“…See supplemental information for full method. Briefly, samples were prepared via the method detailed in McBride et al 50 μL of plasma was mixed with 410 μL of extraction solution (8:1:1 acetonitrile:methanol:acetone) containing an isotopically labeled internal standard mixture (McBride 2019 ). Each sample was vortexed and incubated at 4 °C for 60 min, then centrifuged at 20,000 × g for 10 min at 4 °C in order to pellet the precipitate.…”

Section: Methodsmentioning

confidence: 99%

Discovery of treatment for nerve agents targeting a new metabolic pathway

et al. 2020

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The inhibition of acetylcholinesterase is regarded as the primary toxic mechanism of action for chemical warfare agents. Recently, there have been numerous reports suggesting that metabolic processes could significantly contribute to toxicity. As such, we applied a multi-omics pipeline to generate a detailed cascade of molecular events temporally occurring in guinea pigs exposed to VX. Proteomic and metabolomic profiling resulted in the identification of several enzymes and metabolic precursors involved in glycolysis and the TCA cycle. All lines of experimental evidence indicated that there was a blockade of the TCA cycle at isocitrate dehydrogenase 2, which converts isocitrate to α-ketoglutarate. Using a primary beating cardiomyocyte cell model, we were able to determine that the supplementation of α-ketoglutarate subsequently rescued cells from the acute effects of VX poisoning. This study highlights the broad impacts that VX has and how understanding these mechanisms could result in new therapeutics such as α-ketoglutarate.

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Section: Methodsmentioning

confidence: 99%

Discovery of treatment for nerve agents targeting a new metabolic pathway

et al. 2020

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“…Very often saliva is the preferred body fluid for metabolic studies due to its non-invasive collection method. The LC–MS/MS applied profiling of saliva revealed that differences could be detected in diverse groups of metabolites, such as alterations in the concentrations of steroids, alkaloids, neurotransmitters, and hormones [ 93 ]. Another non-invasively available biofluids is the skin sebum.…”

Section: Human Biofluids Profiling In Health and Diseasementioning

confidence: 99%

Metabolomics and health: from nutritional crops and plant-based pharmaceuticals to profiling of human biofluids

Marchev

Vasileva

Amirova

et al. 2021

Cell. Mol. Life Sci.

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During the past decade metabolomics has emerged as one of the fastest developing branches of “-omics” technologies. Metabolomics involves documentation, identification, and quantification of metabolites through modern analytical platforms in various biological systems. Advanced analytical tools, such as gas chromatography–mass spectrometry (GC/MS), liquid chromatography–mass spectroscopy (LC/MS), and non-destructive nuclear magnetic resonance (NMR) spectroscopy, have facilitated metabolite profiling of complex biological matrices. Metabolomics, along with transcriptomics, has an influential role in discovering connections between genetic regulation, metabolite phenotyping and biomarkers identification. Comprehensive metabolite profiling allows integration of the summarized data towards manipulation of biosynthetic pathways, determination of nutritional quality markers, improvement in crop yield, selection of desired metabolites/genes, and their heritability in modern breeding. Along with that, metabolomics is invaluable in predicting the biological activity of medicinal plants, assisting the bioactivity-guided fractionation process and bioactive leads discovery, as well as serving as a tool for quality control and authentication of commercial plant-derived natural products. Metabolomic analysis of human biofluids is implemented in clinical practice to discriminate between physiological and pathological state in humans, to aid early disease biomarker discovery and predict individual response to drug therapy. Thus, metabolomics could be utilized to preserve human health by improving the nutritional quality of crops and accelerating plant-derived bioactive leads discovery through disease diagnostics, or through increasing the therapeutic efficacy of drugs via more personalized approach. Here, we attempt to explore the potential value of metabolite profiling comprising the above-mentioned applications of metabolomics in crop improvement, medicinal plants utilization, and, in the prognosis, diagnosis and management of complex diseases.

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“…Liquid chromatography-mass spectrometry (LC-MS) is considered the gold standard in metabolomics. In fact, it is able to analyze an enormous range of analytes with a greater sensitivity than NMR [33]. This technique provides very high chromatographic resolution and its results are easily interpretable using libraries of molecular fragmentation patterns [34].…”

Section: Metabolomementioning

confidence: 99%

Saliva and Oral Diseases

Martina

Campanati

Diotallevi

et al. 2020

JCM

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Saliva is a fascinating biological fluid which has all the features of a perfect diagnostic tool. In fact, its collection is rapid, simple, and noninvasive. Thanks to several transport mechanisms and its intimate contact with crevicular fluid, saliva contains hundreds of proteins deriving from plasma. Advances in analytical techniques have opened a new era-called "salivaomics"-that investigates the salivary proteome, transcriptome, microRNAs, metabolome, and microbiome. In recent years, researchers have tried to find salivary biomarkers for oral and systemic diseases with various protocols and technologies. The review aspires to provide an overall perspective of salivary biomarkers concerning oral diseases such as lichen planus, oral cancer, blistering diseases, and psoriasis. Saliva has proved to be a promising substrate for the early detection of oral diseases and the evaluation of therapeutic response. However, the wide variation in sampling, processing, and measuring of salivary elements still represents a limit for the application in clinical practice.

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Rapid liquid chromatography tandem mass spectrometry method for targeted quantitation of human performance metabolites in saliva

Cited by 15 publications

References 46 publications

Discovery of treatment for nerve agents targeting a new metabolic pathway

Discovery of treatment for nerve agents targeting a new metabolic pathway

Metabolomics and health: from nutritional crops and plant-based pharmaceuticals to profiling of human biofluids

Saliva and Oral Diseases

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