Rapid Lc–MS/MS Quantification of The Major Benzodiazepines And Their Metabolites on Dried Blood Spots Using A Simple And Cost-Effective Sample Pretreatment

Déglon, Julien; Versace, François; Lauer, Estelle; Widmer, Christèle; Mangin, Patrice; Thomas, Aurélien; Staub, Christian

doi:10.4155/bio.12.42

Cited by 46 publications

(48 citation statements)

References 35 publications

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“…The current analysis did not take into account investments required for developing a DBS method, which may be more costly than plasma method development. On the other hand, several approaches have been made to speed-up the analytical method such as automated flow-through desorption or reduced sample pre-treatment resulting in a cost-effective analytical method[22, 23]. These technical innovations will further reduce analysis costs in the future.…”

Section: Discussionmentioning

confidence: 99%

Cost Evaluation of Dried Blood Spot Home Sampling as Compared to Conventional Sampling for Therapeutic Drug Monitoring in Children

et al. 2016

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Dried blood spot (DBS) sampling for the purpose of therapeutic drug monitoring can be an attractive alternative for conventional blood sampling, especially in children. This study aimed to compare all costs involved in conventional sampling versus DBS home sampling in two pediatric populations: renal transplant patients and hemato-oncology patients. Total costs were computed from a societal perspective by adding up healthcare cost, patient related costs and costs related to loss of productivity of the caregiver. Switching to DBS home sampling was associated with a cost reduction of 43% for hemato-oncology patients (€277 to €158) and 61% for nephrology patients (€259 to €102) from a societal perspective (total costs) per blood draw. From a healthcare perspective, costs reduced with 7% for hemato-oncology patients and with 21% for nephrology patients. Total savings depend on the number of hospital visits that can be avoided by using home sampling instead of conventional sampling.

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Section: Discussionmentioning

confidence: 99%

Cost Evaluation of Dried Blood Spot Home Sampling as Compared to Conventional Sampling for Therapeutic Drug Monitoring in Children

et al. 2016

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“…However, apart from one recent study by Déglon et al (which determined clobazam and clonaxepam) [23], no recent publication reported on the simultaneous determination of the concentration of multiple AEDs in a single DBS sample. The combined determination of different AEDs has the potential to monitor polymedicated patients and offers the possibility to quantify clinical samples of patients treated with any of these compounds in one sequence, with a single set of calibrators and QC samples [24].…”

Section: Introductionmentioning

confidence: 99%

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Shah

Hawwa

Millership

et al. 2013

Journal of Chromatography B

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Publisher rights This is the author's version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, [VOL 923-924, (2013)] General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The authors wish also to acknowledge the Ministry of Higher Education, Malaysia for the studentship provided. The funder of the current study had no involvement in: (1) design and conduct of the study; (2) collection, management, analysis, and interpretation of the data;and (3) preparation, review, or approval of the manuscript. Conflict of Interest:The authors have no conflicts of interest relevant to this article to disclose.Page 2 Chromatographic separation of the AEDs was achieved using Waters XBridge™ C18 column with a gradient system. The developed method was linear over the concentration ranges studied with r ≥0.995 for all compounds. The lower limits of quantification (LLOQs) were 2, 1, 2, 0.5 and 1 μg/mL for LVT, LTG, PHB, CBZE and CBZ, respectively. Accuracy (%RE) and precision (%CV) values for within and between day were <20% at the LLOQs and <15% at all other concentrations tested. This method was successfully applied to the analysis of the AEDs in DBS samples taken from children with epilepsy for the assessment of their adherence to prescribed treatments. Page 3 of 35A c c e p t e d M a n u s c r i p t 4 Highlights: We report a simple method for the analysis of four antiepileptic drugs in DBS samples  The method was applied to DBS samples collected from children with epilepsy  Such technique has potential in assessing adherence to AEDs using home sampling

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“…Earlier studies on concurrent monitoring of multiple AEDs from one DBS were done mostly with high performance liquid chromatography (HPLC) and included whole blood concentrations of AEDs such as carbamazepine, phenytoin, lamotrigine and barbiturates with limited clinical validation [13], [14]. Recently, a group in North Ireland published a detailed HPLC ultraviolet method for concurrent determination of carbamazepine (CBZ) and its active metabolite carbamazepine-10,11 epoxide (CBZE), levetiracetam (LEV), lamotrigine (LTG) and phenobarbital (PHB) in DBS of children [15].…”

Section: Introductionmentioning

confidence: 99%

Clinical Validation and Implications of Dried Blood Spot Sampling of Carbamazepine, Valproic Acid and Phenytoin in Patients with Epilepsy

et al. 2014

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To facilitate therapeutic monitoring of antiepileptic drugs (AEDs) by healthcare professionals for patients with epilepsy (PWE), we applied a GC-MS assay to measure three AEDs: carbamazepine (CBZ), phenytoin (PHT) and valproic acid (VPA) levels concurrently in one dried blood spot (DBS), and validated the DBS-measured levels to their plasma levels. 169 PWE on either mono- or polytherapy of CBZ, PHT or/and VPA were included. One DBS, containing ∼15 µL of blood, was acquired for the simultaneous measurement of the drug levels using GC-MS. Simple Deming regressions were performed to correlate the DBS levels with the plasma levels determined by the conventional immunoturbimetric assay in clinical practice. Statistical analyses of the results were done using MedCalc Version 12.6.1.0 and SPSS 21. DBS concentrations (Cdbs) were well-correlated to the plasma concentrations (Cplasma): r = 0.8381, 0.9305 and 0.8531 for CBZ, PHT and VPA respectively, The conversion formulas from Cdbs to plasma concentrations were [0.89×CdbsCBZ+1.00]µg/mL, [1.11×CdbsPHT−1.00]µg/mL and [0.92×CdbsVPA+12.48]µg/mL respectively. Inclusion of the red blood cells (RBC)/plasma partition ratio (K) and the individual hematocrit levels in the estimation of the theoretical Cplasma from Cdbs of PHT and VPA further improved the identity between the observed and the estimated theoretical Cplasma. Bland-Altman plots indicated that the theoretical and observed Cplasma of PHT and VPA agreed well, and >93.0% of concentrations was within 95% CI (±2SD); and similar agreement (1∶1) was also found between the observed Cdbs and Cplasma of CBZ. As the Cplasma of CBZ, PHT and VPA can be accurately estimated from their Cdbs, DBS can therefore be used for drug monitoring in PWE on any of these AEDs.

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Rapid Lc–MS/MS Quantification of The Major Benzodiazepines And Their Metabolites on Dried Blood Spots Using A Simple And Cost-Effective Sample Pretreatment

Cited by 46 publications

References 35 publications

Cost Evaluation of Dried Blood Spot Home Sampling as Compared to Conventional Sampling for Therapeutic Drug Monitoring in Children

Cost Evaluation of Dried Blood Spot Home Sampling as Compared to Conventional Sampling for Therapeutic Drug Monitoring in Children

A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots

Clinical Validation and Implications of Dried Blood Spot Sampling of Carbamazepine, Valproic Acid and Phenytoin in Patients with Epilepsy

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