Rapid, label‐free detection of intracranial germinoma using multiphoton microscopy

Abstract

Accurate histopathological diagnosis is essential for facilitating the optimal surgical management of intracranial germinoma. Current intraoperative histological methods are time-and labor-intensive and often produce artifacts. Multiphoton microscopy (MPM) is a label-free imaging technique that can produce intraoperative histological images of fresh, unprocessed surgical specimens. We employ an MPM based on second-harmonic generation and two-photon excited fluorescence microscopy to image fresh, unfixed, and unstained human germinoma specimens. We show that label-free MPM is not only capable of identifying various cells in human germinoma tissue but also capable of revealing the characteristics of germinoma such as granuloma, stromal fibrosis, calcification, as well as the abnormal and uneven structures of blood vessels. In conjunction with custom-developed image-processing algorithms, MPM can further quantify and characterize the extent of stromal fibrosis and calcification. Our results provide insight into how MPM can deliver rapid diagnostic histological data that could inform the surgical management of intracranial germinoma.

Solid pilocytic astrocytoma in cavum septum pellucidum: a description of two cases and an analysis of misdiagnosis

“…Germinoma tumors are classified as grade 4 germ cell tumors in the WHO CNS5 and are extremely aggressive ( 4 ). Germinoma is a highly malignant tumor that is usually found in adolescent, originates from intracranial residual germ cells, and can be multifocal and disseminated in the cerebrospinal fluid ( 18 ). Germinoma tumors in the basal ganglia, saddle area, corpus callosum, and pellucid septum have been reported in the literature, presumably due to the migration of ectopic germ cells from the midline during development ( 19 ).…”

Application of multiphoton microscopy for studying liver fibrosis

“…As described previously, we employed a commercial laser scanning confocal microscopy (LSM 880, Zeiss, Germany) in conjunction with a mode-locked femtosecond Ti: sapphire laser (Chameleon Ultra, Coherent, USA) to conduct multiphoton imaging [8][9][10]. A Plan-Apochromat 20×objective (NA = 0.8, Zeiss, Germany) was employed to acquire multiphoton images.…”

Germinoma supraselar en paciente pediátrico como causa de desnutrición crónica reporte de caso

“…Algunos tumores acompañan reacción granulomatosa que puede confundir con tuberculosis o sarcoidosis y otra celularidad pequeña que plantea diagnóstico diferencial con linfoma y tumor neuro ectodérmico primitivo (Pérez-García, 2007). Sin embargo, también se puede utilizar la microscopía multifotónica para el diagnóstico rápido, que es una técnica de imagen sin etiquetas que puede producir imágenes histológicas intraoperatorias de muestras quirúrgicas frescas y sin procesar (Fang et al, 2019). Por lo general, son negativos (85-90%) para los marcadores tumorales de uso común, como la gonadotropina coriónica humana beta (β-hCG) y la alfafetoproteína (AFP) (Pal et al, 2020).…”