Rapid label-free detection of intact pathogenic bacteria <i>in situ via</i> surface plasmon resonance imaging enabled by crossed surface relief gratings

Nair, Srijit; Gomez-Cruz, Juan; Manjarrez-Hernández, Ángel; Ascanio, Gabriel; Sabat, Ribal Georges; Escobedo, Carlos

doi:10.1039/c9an02339a

Cited by 26 publications

(19 citation statements)

References 48 publications

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“…Novel biosensors have been recently developed based on the unusual plasmonic energy exchange of nanometallic crossed surface-relief gratings (CSRGs). CSRG-based nanosensing, nevertheless, has been restricted to spectroscopic methods and has not exploited its capacity for incorporation with pervasive electronic devices [109]. Nair et al [109] introduced a novel nanosensor using surface plasmon resonance imaging (SPRi) allowed by CSRGs.…”

Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

“…CSRG-based nanosensing, nevertheless, has been restricted to spectroscopic methods and has not exploited its capacity for incorporation with pervasive electronic devices [109]. Nair et al [109] introduced a novel nanosensor using surface plasmon resonance imaging (SPRi) allowed by CSRGs. The imaging system used two-dimensional nanoplasmonic gratings to facilitate a specific transfer of plasmonic energy between metallic nanomaterials.…”

Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

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Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

et al. 2021

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The diagnosis and treatment of urinary tract infections (UTIs) remain challenging due to the lack of convenient assessment techniques and to the resistance to conventional antimicrobial therapy, showing the need for novel approaches to address such problems. In this regard, nanotechnology has a strong potential for both the diagnosis and therapy of UTIs via controlled delivery of antimicrobials upon stable, effective and sustained drug release. On one side, nanoscience allowed the production of various nanomaterial-based evaluation tools as precise, effective, and rapid procedures for the identification of UTIs. On the other side, nanotechnology brought tremendous breakthroughs for the treatment of UTIs based on the use of metallic nanoparticles (NPs) for instance, owing to the antimicrobial properties of metals, or of surface-tailored nanocarriers, allowing to overcome multidrug-resistance and prevent biofilm formation via targeted drug delivery to desired sites of action and preventing the development of cytotoxic processes in healthy cells. The goal of the current study is therefore to present the newest developments for the diagnosis and treatment of UTIs based on nanotechnology procedures in relation to the currently available techniques.

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Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

et al. 2021

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“…As an alternative, CSRGs have also been used for the SPRi of bacteria. Even though the resonant information was not in resolved spatially, Nair et al obtained an LOD of 100 CFU/mL for E. coli by measuring the intensity changes induced by bacterial binding onto CSRGs [55].…”

Section: Plasmonic Imagingmentioning

confidence: 99%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

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Photonic biosensors are a major topic of research that continues to make exciting advances. Technology has now improved sufficiently for photonics to enter the realm of microbiology and to allow for the detection of individual bacteria. Here, we discuss the different nanophotonic modalities used in this context and highlight the opportunities they offer for studying bacteria. We critically review examples from the recent literature, starting with an overview of photonic devices for the detection of bacteria, followed by a specific analysis of photonic antimicrobial susceptibility tests. We show that the intrinsic advantage of matching the optical probed volume to that of a single, or a few, bacterial cell, affords improved sensitivity while providing additional insight into single-cell properties. We illustrate our argument by comparing traditional culture-based methods, which we term macroscopic, to microscopic free-space optics and nanoscopic guided-wave optics techniques. Particular attention is devoted to this last class by discussing structures such as photonic crystal cavities, plasmonic nanostructures and interferometric configurations. These structures and associated measurement modalities are assessed in terms of limit of detection, response time and ease of implementation. Existing challenges and issues yet to be addressed will be examined and critically discussed.

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“…Metallic nanostructures that support surface plasmon resonance (SPR) have been extensively researched in the past decade and employed in several sensing and biosensing applications, including cell analysis [ 1 ], the detection and quantification of infectious diseases [ 2 , 3 , 4 ], and cancer biomarker quantification [ 5 ]. SPR is based on the collective oscillation of conduction electrons, which promotes an enhancement of the electromagnetic field at the metal-dielectric interface [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Minute changes in RI cause a resonance shift in its reflection or transmission spectra, enabling not only the detection but also the quantification of analytes. In the context of (bio)sensing, this phenomenon has found applications in techniques such as SPR spectroscopy [ 1 , 2 , 7 , 8 ], SPR imaging [ 3 , 4 , 9 ], and surface-enhanced Raman scattering (SERS) spectroscopy [ 10 , 11 , 12 ]. SERS is a light scattering-based technique that enables the detection of chemical and biological analytes in a label-free fashion owing to the inherent and unique vibrational modes of the molecules.…”

Section: Introductionmentioning

confidence: 99%

FDTD Analysis of Hotspot-Enabling Hybrid Nanohole-Nanoparticle Structures for SERS Detection

et al. 2022

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Metallic nanoparticles (MNPs) and metallic nanostructures are both commonly used, independently, as SERS substrates due to their enhanced plasmonic activity. In this work, we introduce and investigate a hybrid nanostructure with strong SERS activity that benefits from the collective plasmonic response of the combination of MNPs and flow-through nanohole arrays (NHAs). The electric field distribution and electromagnetic enhancement factor of hybrid structures composed of silver NPs on both silver and gold NHAs are investigated via finite-difference time-domain (FDTD) analyses. This computational approach is used to find optimal spatial configurations of the nanoparticle positions relative to the nanoapertures and investigate the difference between Ag-NP-on-Ag-NHAs and Ag-NP-on-Au-NHAs hybrid structures. A maximum GSERS value of 6.8 × 109 is achieved with the all-silver structure when the NP is located 0.5 nm away from the rim of the NHA, while the maximum of 4.7 × 1010 is obtained when the nanoparticle is in full contact with the NHA for the gold-silver hybrid structure. These results demonstrate that the hybrid nanostructures enable hotspot formation with strong SERS activity and plasmonic enhancement compatible with SERS-based sensing applications.

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Rapid label-free detection of intact pathogenic bacteria in situ via surface plasmon resonance imaging enabled by crossed surface relief gratings

Abstract: The unique plasmonic energy exchange occurring within metallic crossed surface relief gratings (CSRGs) enables Surface Plasmon Resonance Imaging for the label-free detection of whole uropathogenic bacteria.

Cited by 26 publications

References 48 publications

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

FDTD Analysis of Hotspot-Enabling Hybrid Nanohole-Nanoparticle Structures for SERS Detection

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