Rapid label-free colorimetric dual-functional aptasensor for β-lactoglobulin detection based on a rational tailoring strategy

Wang, Xinxin; Chu, Hongjun; Xu, Xiuyuan; Tian, Jingjing; Wu, Yifan; Tian, Hongtao; Zhu, Longjiao; Xu, Wentao

doi:10.1016/j.bios.2022.114223

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…[30] R = CD 265 CD 265 + CD 290 (1) Affinity Determination: The affinity was determined using a previously reported work. [57] Here, 40 μL 3'-biotin labeled enlg2-pl3 (the aptamer of 𝛽-LG) [58] (0.1 μM) and 50 μL streptavidin-magnetic beads (SA-MB) were incubated in 60 μL G4 buffer for 35 min at room temperature. The reaction mixture was washed twice with PBST (0.15 m PBS, 0.05% Tween 20, pH 7.4) and incubated with 40 μL 𝛽-LG (0.01 mg mL −1 ) for 30 min at RT.…”

Section: Methodsmentioning

confidence: 99%

Structural Antagonism‐Aided Conformational Regulation Enables an Aptamer‐Loop G‐Quadruplex Modular Sensor of β‐Lactoglobulin

Wang,

Xu,

Zhu

et al. 2024

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A simple, reliable method for identifying β‐lactoglobulin (β‐LG) in dairy products is needed to protect those with β‐LG allergies. A common, practical strategy for target detection is designing simplified nucleic acid nanodevices by integrating functional components. This work presents a label‐free modular β‐LG aptasensor consisting of an aptamer‐loop G‐quadruplex (G4), the working conformation of which is regulated by conformational antagonism to ensure respective module functionality and the related signal transduction. The polymorphic conformations of the module‐fused sequence are systematically characterized, and the cause is revealed as shifting antagonistic equilibrium. Combined with conformational folding dynamics, this helped regulate functional conformations by fine‐tuning the sequences. Furthermore, the principle of specific β‐LG detection by parallel G4 topology is examined as binding on the G4 aptamer loop by β‐LG to reinforce the G4 topology and fluorescence. Finally, a label‐free, assembly‐free, succinct, and turn‐on fluorescent aptasensor is established, achieving excellent sensitivity across five orders of magnitude, rapidly detecting β‐LG within 22‐min. This study provides a generalizable approach for the conformational regulation of module‐fused G4 sequences and a reference model for creating simplified sensing devices for a variety of targets.

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Section: Methodsmentioning

confidence: 99%

Structural Antagonism‐Aided Conformational Regulation Enables an Aptamer‐Loop G‐Quadruplex Modular Sensor of β‐Lactoglobulin

Wang,

Xu,

Zhu

et al. 2024

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Self Cite

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show abstract

“…13 The aptamers of β-Lg have been screened using the exponentially enriched systematic ligand evolution technique (SELEX). 14 In addition, various techniques have been utilized to develop aptasensors for β-Lg detection, including fluorescence detection, 15,16 surface-enhanced Raman spectroscopy, 17 electrochemistry, 18 and transient synchronous electrophoresis. 19 Hong et al 20 employed a WS 2 nanosheet-based aptasensor for the fluorescence detection of β-Lg with an LOD of 20.4 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%