Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray

Bai, Yanhong; Lu, Yunxing; Wang, Kun; Cheng, Zule; Qu, Youlan; Qiu, Shihui; Zhou, Lin; Wu, Zhenhua; Liu, Huiying; Zhao, Jianlong; Mao, Hongju

doi:10.1007/s40820-019-0285-x

Cited by 47 publications

(37 citation statements)

References 39 publications

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“…72 Bai et al have captured lung cancer EVs through queued beads functionalized with antibodies and combined with quantum dots in a microarray, which showed distinctive lung cancer marker detection level. 73 Immunoaffinity can isolate EV subpopulation, especially for tumor EVs, while limited sample processing volume is a big challenge for the immunoaffinity.…”

Section: Extracellular Vesicle Isolation and Enrichmentmentioning

confidence: 99%

Isolation and Detection Technologies of Extracellular Vesicles and Application on Cancer Diagnostic

Jiang

et al. 2019

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The vast majority of cancers are treatable when diagnosed early. However, due to the elusive trace and the limitation of traditional biopsies, most cancers have already spread widely and are at advanced stages when they are first diagnosed, causing everincreasing mortality in the past decades. Hence, developing reliable methods for early detection and diagnosis of cancer is indispensable. Recently, extracellular vesicles (EVs), as circulating phospholipid vesicles secreted by cells, are found to play significant roles in the intercellular communication as well as the setup of tumor microenvironments and have been identified as one of the key factors in the next-generation technique for cancer diagnosis. However, EVs present in complex biofluids that contain various contaminations such as nonvesicle proteins and nonspecific EVs, resulting in the interference of screening for desired biomarkers. Therefore, applicable isolation and enrichment methods that guarantee scale-up of sample volume, purity, speed, yield, and tumor specificity are necessary. In this review, we introduce current technologies for EV separation and summarize biomarkers toward EV-based cancer liquid biopsy. In conclusion, a novel systematic isolation method that guarantees high purity, recovery rate, and tumor specificity is still missing. Besides that, a dual-model EV-based clinical trial system includes isolation and detection is a hot trend in the future due to efficient point-of-care needs. In addition, cancer-related biomarkers discovery and biomarker database establishment are essential objectives in the research field for diagnostic settings.

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Section: Extracellular Vesicle Isolation and Enrichmentmentioning

confidence: 99%

Isolation and Detection Technologies of Extracellular Vesicles and Application on Cancer Diagnostic

Jiang

et al. 2019

Dose-Response

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“…Exosomes, as circulating biomarkers (exosomal protein, miRNA, and DNA), have received tremendous attention for non‐invasive diagnosis and prognosis of patients with cancer and other diseases. [ 14,110 ] So far, many detection technologies, such as nanoparticle tracking analysis, enzyme‐linked immunosorbent assay (ELISA) and flow cytometry, have been applied to analyze the number, size, and surface proteins or miRNA content of exosomes. [ 12,113,114 ] However, these detection technologies still face many problems, such as the low levels of exosomal markers and the heterogeneous exosome subpopulation, which limit the sensitivity and selectivity of exosome detection.…”

Section: Microfluidics‐based Exosome Detection Strategiesmentioning

confidence: 99%

Microfluidic‐Based Exosome Analysis for Liquid Biopsy

et al. 2021

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Liquid biopsy offers non‐invasive and real‐time molecular profiling of individual patients, and is thus considered a revolutionary technology in precision medicine. Exosomes have been acknowledged as significant biomarkers in liquid biopsy, as they play a central role in cell–cell communication and are closely related to the pathogenesis of most human malignancies. Nevertheless, in biofluids exosomes always co‐exist with other particles, and the cargo components of exosomes are highly heterogeneous. Thus, the isolation and molecular characterization of exosomes are still technically challenging. Microfluidics technology effectively addresses this challenge by virtue of its inherent advantages, such as precise manipulation of fluids, low consumption of samples and reagents, and a high level of integration. Recent advances in microfluidics allow in situ exosome capture and molecular detection with unprecedented selectivity and sensitivity. In this review, the state‐of‐the‐art developments in microfluidics‐based exosome research, including exosome isolation approaches and molecular detection strategies, with highlights of the characterization of exosomal biomarkers in cancer liquid biopsy is summarized. The major challenges are also discussed and some perspectives for the future directions of exosome‐based liquid biopsy in microfluidic systems are presented.

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“…By applying immune-based capture technique for the CSPG4 epitope, the authors identified a specific protein profile of MTEX that was qualitatively and quantitatively distinct from normal cell-derived exosomes in plasma of patients with melanoma. In a similar way, Bai et al (185) recently developed an immuno-based microfluidic chip using a quantum dot multiplex detection system to characterize lung cancer exosomes for the expression of different cancer-related surface molecules.…”

Section: Extracellular Vesicles As Fingerprint Of Tumor Metabolic Statementioning

confidence: 99%

Intrinsic and Extrinsic Modulators of the Epithelial to Mesenchymal Transition: Driving the Fate of Tumor Microenvironment

et al. 2020

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The epithelial to mesenchymal transition (EMT) is an evolutionarily conserved process. In cancer, EMT can activate biochemical changes in tumor cells that enable the destruction of the cellular polarity, leading to the acquisition of invasive capabilities. EMT regulation can be triggered by intrinsic and extrinsic signaling, allowing the tumor to adapt to the microenvironment demand in the different stages of tumor progression. In concomitance, tumor cells undergoing EMT actively interact with the surrounding tumor microenvironment (TME) constituted by cell components and extracellular matrix as well as cell secretome elements. As a result, the TME is in turn modulated by the EMT process toward an aggressive behavior. The current review presents the intrinsic and extrinsic modulators of EMT and their relationship with the TME, focusing on the non-cell-derived components, such as secreted metabolites, extracellular matrix, as well as extracellular vesicles. Moreover, we explore how these modulators can be suitable targets for anticancer therapy and personalized medicine.

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Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray

Cited by 47 publications

References 39 publications

Isolation and Detection Technologies of Extracellular Vesicles and Application on Cancer Diagnostic

Isolation and Detection Technologies of Extracellular Vesicles and Application on Cancer Diagnostic

Microfluidic‐Based Exosome Analysis for Liquid Biopsy

Intrinsic and Extrinsic Modulators of the Epithelial to Mesenchymal Transition: Driving the Fate of Tumor Microenvironment

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