Rapid isolation and detection of aquaculture pathogens in an integrated microfluidic system using loop-mediated isothermal amplification

Chang, Wen Hsin; Yang, Sung Yi; Wang, Chih‐Hung; Tsai, Ming An; Wang, Pei Chi; Chen, Tzong-Yueh; Chen, Shih Chu; Lee, Gwo‐Bin

doi:10.1016/j.snb.2011.12.054

Cited by 55 publications

(34 citation statements)

References 37 publications

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“…1(b)). Then, a magnet in the integrated control system (Chang et al, 2013) was moved to the region under a reaction chamber automatically for collection of bacteria-magnetic beads complexes ( Fig. 1(c)).…”

Section: Methodsmentioning

confidence: 99%

“…10 mL of KAPA SYBR FAST PCR master mix, 0.4 mL of 10 mM forward primer, 0.4 mL of 10 mM reverse primer and 9.2 mL of distilled water constituted 20 mL of PCR reaction mixture which was preloaded in the reagent chamber on the microfluidic chip before the whole assay. The thermal-cycling protocol pre-set in the control software was 95°C for 5 min, followed by 25 cycles of 95°C for 10 s, 58°C for 20 s and 72°C for 10 s. The double-strand PCR products intercalated by fluorescent materials in the KAPA SYBR FAST PCR master mix were then detected by a fluorescent detection module on the integrated control system (Chang et al, 2013).…”

Section: Pcr Primers and Pcr Reactionmentioning

confidence: 99%

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Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

Chang

Wang

Lin

et al. 2015

Biosensors and Bioelectronics

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Section: Methodsmentioning

confidence: 99%

Section: Pcr Primers and Pcr Reactionmentioning

confidence: 99%

Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

Chang

Wang

Lin

et al. 2015

Biosensors and Bioelectronics

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“…Other types of negative-pressure-driven microfluidic components can be found in the literature. [27][28][29] The normally closed valve and micromixer were controlled and driven using a vacuum pump connected to an electromagnetic valve (EMV), and fluid transport was achieved using a syringe pump (Legato 180, KD Scientific Inc., Holliston, MA) connected to the waste outlet channel that was driven with a withdrawing force by opening the inlet to atmospheric pressure. Using a personal computer, control of the fluid in the microchannel can be performed automatically.…”

Section: Methodsmentioning

confidence: 99%

A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay

et al. 2013

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This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro-mixer, were driven by negative pressure, which simplifies the use of the chip and facilitates commercialization. Magnetic beads were used as a solid support for the primary antibody, which captured apolipoprotein A1 in patients' urine. Because of the three-dimensional structure of the magnetic beads, the concentration range of the target that could be detected was as high as 2000 ng ml À1 . Because this concentration is 100 times higher than that quantifiable using a 96-well plate with the same enzyme-linked immunosorbent assay (ELISA) kit, the dilution of the patient's urine can be avoided or greatly reduced. The limit of detection was determined to be approximately 10 ng ml À1 , which is lower than the cutoff value for diagnosing bladder cancer (11.16 ng ml À1 ). When the values measured using the microfluidic chip were compared with those measured using conventional ELISA using a 96-well plate for five patients, the deviations were 0.9%, 6.8%, 9.4%, 1.8%, and 5.8%. The entire measurement time is 6-fold faster than that of conventional ELISA. This microfluidic device shows significant potential for point-ofcare applications. V C 2013 American Institute of Physics.

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“…Liposomes with reporter probes and beads with capture probes were incubated with amplified targets prior to introduction of the mixture into a microchannel, where the sandwich complexes were subsequently captured on the magnet and detected by means of fluorescence microscopy. Chang et al (2012) reported an integrated microfluidic system for detection of aquaculture pathogens, which contained microvalves, micropumps, reaction chambers, and washing units, such that the process for isolation of pathogen DNA, nucleic acid amplification, and optical detection was performed in automated manner. The DNA of the target pathogen was first isolated by magnetic beads coated with specific nucleotide probes after cell lysis.…”

Section: Magnetic Particles Coupled With Nucleic Acid Detection (Pcr mentioning

confidence: 99%

Microfluidic applications of functionalized magnetic particles for environmental analysis: focus on waterborne pathogen detection

Ramadan

Gijs

2012

Microfluid Nanofluid

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The continuous surveillance of drinking water is extremely important to provide early warning of contamination and to ensure continuous supplies of healthy drinking water. Isolation and detection of a particular type of pathogen present at low concentration in a large volume of water, concentrating the analyte in a small detection volume, and removing detection inhibiting factors from the concentrated sample, present the three most important challenges for water quality monitoring laboratories. Combining advanced biological detection methods (e.g., nucleic acid-based or immunology-based protocols) with microfluidics and immunomagnetic separation techniques that exploit functionalized magnetic particles has tremendous potential for realization of an integrated system for pathogen detection, in particular, of waterborne pathogens. Taking advantage of the unique properties of magnetic particles, faster, more sensitive, and more economical diagnostic assays can be developed that can assist in the battle against microbial pathogenesis. In this review, we highlight current technologies and methods used for realization of magnetic particle-based microfluidic integrated waterborne pathogen isolation and detection systems, which have the potential to comply in future with regulatory water quality monitoring requirements.

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Rapid isolation and detection of aquaculture pathogens in an integrated microfluidic system using loop-mediated isothermal amplification

Cited by 55 publications

References 37 publications

Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay

Microfluidic applications of functionalized magnetic particles for environmental analysis: focus on waterborne pathogen detection

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