Rapid iodometric detection of Aeromonas amylase and its diagnostic significance

Emele, Felix Emele

doi:10.1016/s0732-8893(01)00257-7

Cited by 4 publications

(2 citation statements)

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“…DNase activity has been observed in Aeromonas strains from frozen fish (Castro‐Escarpulli and others 2003) and food samples (Yücel and Erdogan 2010). Emele (2001) has reported amylase activity in all the A. hydrophila isolates from different sources (food, clinical, and environmental). In an earlier study, 96% of Aeromonas spp.…”

Section: Resultsmentioning

confidence: 99%

Prevalence, Characterization, and Antimicrobial Resistance of Aeromonas Strains from Various Retail Food Products in Mumbai, India

Nagar¹,

Shashidhar²,

Bandekar³

2011

Journal of Food Science

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A total of 154 food samples (chicken, fish, and ready-to-eat sprouts) from various retail outlets in Mumbai, India, were analyzed for the presence of Aeromonas spp. over a period of 2 y (January 2006 to March 2008). Twenty-two Aeromonas isolates belonging to 7 different species were isolated from 18 (11.7%) food samples. The highest percentages of isolation were from chicken (28.6%) followed by fish (20%) and sprout (2.5%) samples. Aeromonas caviae, A. veronii bv. sobria, and A. salmonicida were the most frequently isolated species from sprouts, chicken, and fish samples, respectively. The genes encoding for putative virulence factors, cytotoxic enterotoxin (act), hemolysin (hly), aerolysin (aer), elastase (ahyB), and lipase (lip) were detected using polymerase chain reaction method in 59.1%, 40.9%, 22.7%, 54.5%, and 31.8% of the strains, respectively. The isolated Aeromonas strains were found to be positive for virulence factors, that is, amylase, DNase, gelatinase, protease, and lipase production. More than 60% isolates were also positive for β-hemolytic activity. All these food isolates were found to be resistant to ampicillin and bacitracin, and sensitive to gentamicin, 3rd-generation cephalosporins (ceftazidime, cephotaxime, ceftriaxone), and chloramphenicol. Seventeen (77.2%) isolates harbored single and/or multiple plasmids (approximately 5 to >16 kb). The XbaI digestion patterns of chromosomal DNA of these isolates, using pulsed field gel electrophoresis, showed high genetic diversity among these isolates. Our results demonstrate the presence of various Aeromonas spp. with virulence potential and antimicrobial resistance in different food products marketed in Mumbai, India. The potential health risks posed by consumption of these raw or undercooked food products should not be underestimated.

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Section: Resultsmentioning

confidence: 99%

Prevalence, Characterization, and Antimicrobial Resistance of Aeromonas Strains from Various Retail Food Products in Mumbai, India

Nagar¹,

Shashidhar²,

Bandekar³

2011

Journal of Food Science

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“…Amylase activity, although considered a virulence factor (Campbell et al 1990;Pemberton et al 1997;Emele, 2001), is usually described only in taxonomic studies (Miñana-Galbis et al 2002;Valera and Esteve, 2002). In our case the percentage of strains showing this activity was 97 % (93.1-100%, depending on the species), with only two A. hydrophila (AE215, AE216) and the A. salmonicida subsp.…”

Section: R a F Tmentioning

confidence: 49%

Potential pathogenicity ofAeromonas hydrophilacomplex strains isolated from clinical, food, and environmental sources

et al. 2016

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Aeromonas are autochthonous inhabitants of aquatic environments, including chlorinated and polluted waters, although they can also be isolated from a wide variety of environmental and clinical sources. They cause infections in vertebrates and invertebrates and are considered to be an emerging pathogen in humans, producing intestinal and extra-intestinal diseases. Most of the clinical isolates correspond to A. hydrophila, A. caviae, and A. veronii bv. Sobria, which are described as the causative agents of wound infections, septicaemia, and meningitis in immunocompromised people, and diarrhoea and dysenteric infections in the elderly and children. The pathogenic factors associated with Aeromonas are multifactorial and involve structural components, siderophores, quorum-sensing mechanisms, secretion systems, extracellular enzymes, and exotoxins. In this study, we analysed a representative number of clinical and environmental strains belonging to the A. hydrophila species complex to evaluate their potential pathogenicity. We thereby detected their enzymatic activities and antibiotic susceptibility pattern and the presence of virulence genes (aer, alt, ast, and ascV). The notably high prevalence of these virulence factors, even in environmental strains, indicated a potential pathogenic capacity. Additionally, we determined the adhesion capacity and cytopathic effects of this group of strains in Caco-2 cells. Most of the strains exhibited adherence and caused complete lysis.

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