Rapid investigation of α-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting

Abstract

Phaleria macrocarpa, known as "Mahkota Dewa", is a widely used medicinal plant in Malaysia. This study focused on the characterization of α-glucosidase inhibitory activity of P. macrocarpa extracts using Fourier transform infrared spectroscopy (FTIR)-based metabolomics. P. macrocarpa and its extracts contain thousands of compounds having synergistic effect. Generally, their variability exists, and there are many active components in meager amounts. Thus, the conventional measurement methods of a single component for the quality control are time consuming, laborious, expensive, and unreliable. It is of great interest to develop a rapid prediction method for herbal quality control to investigate the α-glucosidase inhibitory activity of P. macrocarpa by multicomponent analyses. In this study, a rapid and simple analytical method was developed using FTIR spectroscopy-based fingerprinting. A total of 36 extracts of different ethanol concentrations were prepared and tested on inhibitory potential and fingerprinted using FTIR spectroscopy, coupled with chemometrics of orthogonal partial least square (OPLS) at the 4000-400 cm frequency region and resolution of 4 cm. The OPLS model generated the highest regression coefficient with RY = 0.98 and QY = 0.70, lowest root mean square error estimation = 17.17, and root mean square error of cross validation = 57.29. A five-component (1+4+0) predictive model was build up to correlate FTIR spectra with activity, and the responsible functional groups, such as -CH, -NH, -COOH, and -OH, were identified for the bioactivity. A successful multivariate model was constructed using FTIR-attenuated total reflection as a simple and rapid technique to predict the inhibitory activity.

Investigation of α-Glucosidase Inhibitory Metabolites from Tetracera scandens Leaves by GC–MS Metabolite Profiling and Docking Studies

“…On the other hand, the α-glucosidase inhibitory activity followed a different trend, with the lowest potential attributed to the water extract (IC 50 = 95.32 ± 2.32 µg/mL) followed by the 20% methanol extract (IC 50 = 56.56 ± 1.68 µg/mL), while the highest activity (IC 50 = 19.54 ± 2.5 µg/mL to 23.46 ± 3.91 µg/mL) was exhibited by the 40%, 60%, 80% and 100% methanol extracts, which were not significantly different from each other at p < 0.05. These results are consistent with [11,23,28] who have reported that the higher α-glucosidase inhibitory activity was associated with the higher alcohol to water ratios of the extractant mixtures, and vice versa.…”

“…Moreover, the orthogonal component t 0 [1] further separates the methanol extract from the other highly active extracts, which may indicate compositional differences between methanol extract and the other highly active extracts. The developed model was considered valid since the values of R 2 Y (cum) and Q 2 Y (cum) were above 0.5 (0.973 and 0.921, respectively) and the values of root mean square error of estimation (RMSEE) and root mean square error of cross validation (RMSEcv) were low (5.118 and 7.850, respectively) [4,28,29]. The supplementary plot ( Figure S1…”

Phytochemical characterization and cancer cell line cytotoxicity of Clitoria ternatea

“…A peak at 2922 cm -1 was assigned as alkane asymmetric C-H stretching vibration of methyl and methylene group. The peak 2852 cm -1 represented to sp2 and sp3 stretching of C-H bonds of aldehyde and methyl group was predicted (Easmin et al, 2017). The C -O valence vibration of primary alcohols and polysaccharide were observed at 1066 cm -1 (Mak et al, 2013).…”

Synthesis of natural nanopesticides with the origin of Eucalyptus globulus extract for pest control

“…The FTIR spectrum of the pure Eucalyptus extract and nanocapsule (F4) recorded in the 4000-500 cm −1 range is indicated in Figure 1(a, b). The Figure 1(a) shows the assigned to the C-X (Halogen; Vibration of halogen and out of plane) (53). Figure 1( (54).…”